(1) This change in cholesterol esterification appears to be the result of reductions in the activity of acyl-CoA:cholesterol acyltransferase (ACAT) in the endoplasmic reticulum of the macrophages incubated with the n-3 polyunsaturated fatty acids.
(2) Acaterin also inhibited ACAT activity in the rat liver microsomes by 50% at a concentration of 120 microM.
(3) This mechanism, which is activated by changes in culture density, coordinately regulates the activities of HMG-CoA reductase and acyl-CoA:cholesterol acyltransferase (ACAT).
(4) These results suggest that ACAT activity in microsomes was affected not only by the amount of microsomal cholesterol, but also by an extra-microsomal soluble protein such as lipoproteins.
(5) ACAT activity was also determined in microsomes prepared from twenty human liver samples obtained at surgery with in vivo warm ischaemia times ranging from 5-120 min.
(6) In sterol-free medium, if sterol synthesis is blocked by specific enzyme inhibitors or through mutation, the ACAT activation by cycloheximide is again abolished.
(7) Kinetic studies indicate that DL-MA is an uncompetitive inhibitor of ACAT.
(8) The results suggest that ACAT activity in CaCo-2 cells is stimulated by cholesterol delivered to the cells by way of taurocholate micelles.
(9) Furthermore, the large beta-VLDL had a higher ACAT-stimulatory potential than the smaller beta-VLDL.
(10) A decrease in HMG-CoA reductase and an increase ACAT activity was observed compared with the corresponding values from both the groups fed on a standard diet and a fat supplemented diet with no cholesterol.
(11) The IC50 values of enniatins D, E and F for ACAT activity in an enzyme assay using rat liver microsomes were calculated to be 87, 57 and 40 microM, respectively.
(12) Except in adult rats, the HG diet always raises hepatic ACAT activity more than the HCO diet or the Purina Chow.
(13) In contrast, ACAT activity in NPD fibroblasts (cell lines from four different patients) began to increase between 6 and 12 h after serum addition, reaching levels up to 50% of normal values at 24 h. ACAT activity in NPC and NPD cell extracts could not be stimulated by preincubation with normal cell homogenates, nor was complementation between NPC and NPD homogenates observed.
(14) Apparent ACAT activity levels increased significantly in nude mouse tumours and in foetal and postnatal rat pancreata and also in postnatal liver.
(15) A 5% cholesterol diet for 3 hr produced an increase in the microsomal and plasmatic cholesterol content, a decrease in HMG-CoA reductase activity and a concomitant increase in ACAT activity.
(16) The effect of CDCA, UDCA and the cyclopropyl analogues was also tested with respect to HMG-CoA reductase and acylCoA cholesterol acyltransferase (ACAT) activities.
(17) The structure of purpactins, novel acyl-CoA:cholesterol acyltransferase (ACAT) inhibitors, was determined by spectroscopic analyses.
(18) The enhanced ACAT activity obtained by freezing was at least partly explained by a transfer of unesterified cholesterol to the microsomal fraction and possibly also by making the substrate(s) more available to the enzyme.
(19) Under these experimental conditions, treatment with insulin or with the ACAT inhibitor CL-277082 significantly reduced the plasma cholesterol to levels measured in nondiabetic rats fed the same high fat diet.
(20) DL-MA inhibited acyl CoA:cholesterol acyltransferase (ACAT, EC 2.3.1.26) activity in the mucosal microsomes, with 50% inhibition occurring at approximately 0.5 microM.
(1) Cate Blanchett and Laura Linney both paid tribute to Rowlands, calling her a trailblazer and inspiration.
(2) Isofunctional beta-ketoadipate:succinyl coenzyme A transferases I and II are encoded by the pcaE and catE genes, respectively, of Acinetobacter calcoaceticus.
(3) Click here for the Magic in the Moonlight trailer Compared with the gloomy ruminations on ageing and aspiration that characterised the well-received Blue Jasmine, which won Cate Blanchett an Oscar , this is Allen going back to the knockabout farce and blithe May-December couplings that populate his lighter films.
(4) Their 12-year stewardship transformed an obscure theatre notorious for the austerity of its seats into a fashionable address renowned for its rollcall of stars - including Ralph Fiennes, Diana Rigg, Juliet Binoche and Cate Blanchett - all of whom were eager to muck in with communal dressing rooms and a minimum wage.
(5) Speaking about his most recent release, in which Cate Blanchett plays Jasmine, a socialite whose luxurious New York life abruptly ends with the suicide of her corrupt financier husband, Allen said he felt more comfortable writing serious roles for women, rather than men.
(6) By using methanol-0.15 M borate buffer of pH 8.0, cate-chol-O-methyltransferase activity might be assayed.
(7) Removal of the DNA between the SalI sites created a deletion removing the terminal 35 base pairs of the catB gene, the 300-base-pair catC gene, and about 1.1 kb of the 1.2-kb catE gene.
(8) Cate Blanchett gave one of these addresses just a week ago and you’re stuck with me.” Thorpe went on to congratulate the graduates on their achievement, adding the transition from student to working life would take a “steely determination”.
(9) These sequences are closely related to sequences from its human analogue, which we recently cloned and expressed (Wallner, B. P., Mattaliano, R. J., Hession, C., Cate, R. L., Tizard, R., Sinclair, L. K., Foeller, C., Chow, E. P., Browning, J. L., Ramachandran, K. L., and Pepinsky, R. B.
(10) Cate Blanchett alluded to the problem in accepting the best actress Oscar for Blue Jasmine.
(11) The respective genes encode enzymes that catalyze four consecutive reactions in the catechol branch of the beta-ketoadipate pathway: catB, muconate lactonizing enzyme (EC 5.5.1.1); catC, muconolactone isomerase (EC 5.3.3.4); catD, beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24); and catE, beta-ketoadipate succinyl-coenzyme A transferase (EC 2.8.3.6).
(12) The film has already attracted some Oscar buzz; Cannes is likely to be a launchpad for a number of awards campaigns, including one for Cate Blanchett, who stars in Carol, Todd Haynes’s adaptation of the Patricia Highsmith novel about a clandestine lesbian love affair in 1950s New York.
(13) Cate Blanchett has found herself in the midst of a climate change row after appearing in a TV commercial calling on Australians to back a tax on carbon.
(14) But the prolific film-maker –with his latest comedy-drama Blue Jasmine starring Cate Blanchett as a pill-popping socialite - has admitted that while making films isn't difficult, making good ones can be.
(15) As awareness increases it exposes some negative views, and there’s often a lashing out by some members of the community at trans people.” We were also seen as dangerous by our fellow gay people Mark Gillespie She cites vilification of the writer and soldier Cate McGregor by some commentators and bloggers.
(16) In the final race of the night, Australia’s women, led by strong swims from the Campbell sisters, Bronte and Cate, secured gold in a world record time of three minutes, 30.65.
(17) Then Cate Blanchett, whom Allen directed in Blue Jasmine .
(18) Woody Allen and Cate Blanchett have issued statements in response to t he open letter posted by Allen's adopted daughter, Dylan, on a New York Times blog .
(19) W. Cates, Jr., of the U.S. Center for Disease Control reported recently that earlier abortions run less risk of complications or mortality.
(20) Photograph: Sony Pictures Classics Truth review: Blue Jasmine in the 60 Minutes newsroom, with a twist Read more Zodiac screenwriter James Vanderbilt’s directorial debut about the Rathergate scandal that ended the careers of veteran CBS newsman Dan Rather (played by Robert Redford) and 60 Minutes producer Mary Mapes (Cate Blanchett) has been eclipsed by Spotlight , this year’s other journalistic thriller.