What's the difference between affinity and dye?

Affinity


Definition:

  • (n.) Relationship by marriage (as between a husband and his wife's blood relations, or between a wife and her husband's blood relations); -- in contradistinction to consanguinity, or relationship by blood; -- followed by with, to, or between.
  • (n.) Kinship generally; close agreement; relation; conformity; resemblance; connection; as, the affinity of sounds, of colors, or of languages.
  • (n.) Companionship; acquaintance.
  • (n.) That attraction which takes place, at an insensible distance, between the heterogeneous particles of bodies, and unites them to form chemical compounds; chemism; chemical or elective affinity or attraction.
  • (n.) A relation between species or highe/ groups dependent on resemblance in the whole plan of structure, and indicating community of origin.
  • (n.) A superior spiritual relationship or attraction held to exist sometimes between persons, esp. persons of the opposite sex; also, the man or woman who exerts such psychical or spiritual attraction.

Example Sentences:

  • (1) The low affinity of several N1-alkylpyrroleethylamines suggests that the benzene portion of the alpha-methyltryptamines is necessary for significant affinity.
  • (2) These results demonstrate that increased availability of galactose, a high-affinity substrate for the enzyme, leads to increased aldose reductase messenger RNA, which suggests a role for aldose reductase in sugar metabolism in the lens.
  • (3) It has been generally believed that the ligand-binding of steroid hormone receptors triggers an allosteric change in receptor structure, manifested by an increased affinity of the receptor for DNA in vitro and nuclear target elements in vivo, as monitored by nuclear translocation.
  • (4) Because cystine in medium was converted rapidly to cysteine and cysteinyl-NAC in the presence of NAC and given that cysteine has a higher affinity for uptake by EC than cystine, we conclude that the enhanced uptake of radioactivity was in the form of cysteine and at least part of the stimulatory effect of NAC on EC glutathione was due to a formation of cysteine by a mixed disulfide reaction of NAC with cystine similar to that previously reported for Chinese hamster ovarian cells (R. D. Issels et al.
  • (5) We sought additional evidence for an inverse relationship between functional CTL-target cell affinity on the one hand, and susceptibility of the CTL-mediated killing to inhibition by alpha LFA-1 and alpha Lyt-2,3 monoclonal antibodies on the other hand.
  • (6) This theory was confirmed by product analysis and by measuring the affinity of the substrate for the enzyme by its inhibition of p-nitrophenyl glucoside hydrolysis.
  • (7) We report a series of experiments designed to determine if agents and conditions that have been reported to alter sodium reabsorption, Na-K-ATPase activity or cellular structure in the rat distal nephron might also regulate the density or affinity of binding of 3H-metolazone to the putative thiazide receptor in the distal nephron.
  • (8) ASF-II was purified to apparent homogeneity by using concanavalin A-agarose affinity chromatography, DEAE-cellulose chromatography, alumina gel adsorption, and isoelectric focussing techniques.
  • (9) The occupation of the high affinity calcium binding site by Ca(II) and Mn(II) does not influence the Cu(II) binding process, suggesting that there is no direct interaction between this site and the Cu(II) binding sites.
  • (10) Radioligand binding studies revealed the presence of a single class of high-affinity (Kd = 2-6 X 10(-10) M) binding sites for ET-1 in both cells, although the maximal binding capacity of cardiac receptor was about 6- to 12-fold greater than that of vascular receptor.
  • (11) The penicillin-resistant Enterococcus hirae R40 has a typical profile of membrane-bound penicillin-binding proteins (PBPs) except that the 71 kDa PBP5 of low penicillin affinity represents about 50% of all the PBPs present.
  • (12) The enzyme was solubilized by Triton X-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-AH-Sepharose 4B.
  • (13) Only estrogenic hormones are bound with high affinity.
  • (14) These results indicate that both the renal brush-border and basolateral membranes possess the Na(+)-dependent dicarboxylate transport system with very similar properties but with different substrate affinity and transport capacity.
  • (15) This novel mechanism of receptor regulation, named transmodulation, should be distinguished from the reduction in total receptor number caused by the homologous ligand (downregulation) and from the change in affinity produced by the binding of agonists or antagonists to the same receptor site.
  • (16) Neurotensin (NT) is an endogenous brain tridecapeptide for which high affinity binding sites exist in the central nervous system.
  • (17) In lactate medium the capacity of each AIB carrier is unchanged but its affinity is reduced to one-third.
  • (18) The telencephalon of teleost fish shows high affinity uptake for D-[3H]aspartate, intermediate levels of GABAergic markers and low levels of cholinergic enzymes.
  • (19) In all immunized rabbits the antisera obtained with the 7 alpha-derivative had a higher affinity and a narrower specificity than the antiserum obtained with the 7 beta-derivative.
  • (20) The binding parameters indicate that the principal activating effect of UMP is not simply to increase the affinity of the enzyme for glucose.

Dye


Definition:

  • (v. t.) To stain; to color; to give a new and permanent color to, as by the application of dyestuffs.
  • (n.) Color produced by dyeing.
  • (n.) Material used for dyeing; a dyestuff.
  • (n.) Same as Die, a lot.

Example Sentences:

  • (1) The most successful dyes were phenocyanin TC, gallein, fluorone black, alizarin cyanin BB and alizarin blue S. Celestin blue B with an iron mordant is quite successful if properly handled to prevent gelling of solutions.
  • (2) The actions of the polyvalent cationic dye Ruthenium Red and the enzyme neuraminidase were studied at the frog neuromuscular junction.
  • (3) Significant increases in the extravasation of dye were observed in both animal groups sensitized with IgG1 and IgG2 antibodies.
  • (4) While the reduced form of the "derived" polyphenolic compounds, generated during tissue homogenization, appeared to enhance dye binding with bovine serum albumin, their influence on the protein assay directly in crude homogenates was extremely diverse.
  • (5) To selectively stain polyanionic macromolecules of growth plate cartilage and to prevent artifacts induced by aqueous fixation, proximal tibial growth plates were excised from rats, slam-frozen, and freeze-substituted in 100% methanol containing the cationic dye Alcian blue.
  • (6) This dye is concentrated and secreted by the parietal cells.
  • (7) The rhodamine 123-induced growth inhibition was partially reversed by treating the dye-pre-exposed infected erythrocytes with the proton ionophore carbonyl-cyanide m-chlorophenylhydrazone, which dissipates transmembrane proton gradients.
  • (8) The duration of electrophoresis was based on the migration of a marker dye for a predetermined distance.
  • (9) Effects of fixation with glutaraldehyde (GA), glutaraldehyde-osmium tetroxide (GA-OsO(4)), and osmium tetroxide (OsO(4)) on ion and ATP content, cell volume, vital dye staining, and stability to mechanical and thermal stress were studied in Ehrlich ascites tumor cells (EATC).
  • (10) By using pH- or Ca(2+)-sensitive dyes and recording at the ion-sensitive and -insensitive (isosbestic) wavelengths, the method can measure both cell volume changes and intracellular ionic activities.
  • (11) An argon dye laser system with lambda em=630 nm (400 mW cm-2) was used for PDT with a total light dose of 400 J cm-2.
  • (12) In a complete system, consisting of a dye-donor couple, ferredoxin, thioredoxin and ferredoxin-thioredoxin reductase, light activation of purified spinach fructose-1,6-bisphosphatase was observed in vitro.
  • (13) When given 30 min after acetic acid instillation SC-41930 prevented the rise in myeloperoxidase and dye extravasation observed in the acetic acid inflammed tissue.
  • (14) A comparison was made between the Q's estimated by the CO2 rebreathing method during tethered swimming and previously published data on Q determined by the dye-dilution method during free swimming in a flune.
  • (15) A novel staining procedure for enumerating osteoclasts on neonatal mouse calvaria with the vital fluorescent dye acridine orange is described.
  • (16) Thus, angiographic dye appears to decrease heart rate by a direct effect on pacemaker tissue and by reflex vagal suppression of the sinus pacemaker.
  • (17) The purpose of this study was to compare the level of apical dye penetration when different sealers were used.
  • (18) This protein which we call CBP-58 bears similarities to the endoplasmic reticulum protein, calreticulin, in that it has a pI of 4.7 containing approximately 30% glutamate and aspartate, has a high capacity for calcium, and stains blue with the carbocyanine dye, 'Stains-all'.
  • (19) Minced and triturated fragments from the spinal cord of normal rat fetuses (15-18 days gestation) labeled with the fluorescent dye fast blue (FB) were successfully transplanted into juvenile myelin-deficient rat spinal cord under direct observation.
  • (20) In vitro, the soluble core PEI and membrane both bound reactive substances of limited aqueous stability, such as from [14C]N-methyl-N-nitrosourea ([14C]NMU), and aqueous stable dyes of molecular weight up to 1000.