(v. t.) To confide (one's self to, or in); to trust.
(v. t.) To betroth or espouse; to affiance.
(v. t.) To bind in faith.
(v. i.) To trust or confide.
Example Sentences:
(1) Purification of the new conjugate was performed in three sequential steps: (1) by HPLC gel filtration on TSK G3000SW to remove the unconjugated ricin: (2) by affinity chromatography on Affi-Gel Blue to separate the free antibody from the conjugate and (3) by affinity chromatography on Sepharose 6B to separate the galactose-binding IT from the non-binding moiety.
(2) Calcium-dependent binding to phenothiazine Affi-Gel confirmed that calcium binding induces conformation changes characteristic of calmodulin.
(3) In order to increase understanding of the control of inositol-1,3,4-trisphosphate kinase activity, the enzyme was highly purified from rat liver by precipitation with polyethylene glycol, MonoQ ion-exchange chromatography, heparin-agarose affinity chromatography, and a novel affinity chromatography procedure that utilized Affi-Gel resin to which InsP6 was coupled (Marecek, J.F., and Prestwich, G.D. (1991) Tetrahedron Lett.
(4) An affinity-purified fraction (APF) was obtained by passing crude somatic antigens of Trichinella spiralis muscle larvae through an Affi-Gel 10 column coupled with anti-Trichuris suis IgG.
(5) Rabbit liver tRNA nucleotidyldransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA.
(6) A thiol peptidase that catalyzes at near neutral pH the hydrolysis of insulin, the isolated A and B chains of insulin, and glucagon was purified from rat liver cytosol by fractionation on Sephadex G-200, Affi-Gel Blue, and Spherogel TSK-G 3000 SW.
(7) Purification involved sonic disruption of bacterial cells, ammonium sulfate fractionation, chromatography on Affi-Gel Blue, chromatofocusing, and gel filtration on Sephadex G-100.
(8) The versatility of the Affi-Gel-10 monomer affinity matrix was further demonstrated by purifying 13 mammalian arylsulfatase A enzymes to homogeneity, as assessed by Sephacryl chromatography, native and SDS gel electrophoresis.
(9) Pooled serum from normal cattle and sheep and serum from sheep, infected with two different viruses, were prepared for HPIEC by chromatography on CM-Affi-Gel Blue.
(10) A chalcone derived from a potent retinobenzoic acid (Ch55) was chosen as the ligand and fixed to an immobilized matrix by coupling with the N-hydroxysuccinimide ester of agarose (Affi-Gel 10, Bio-Rad Laboratories).
(11) The purpose of this study was to characterize a suppressive active glycopeptide (SAP) using affinity chromatography (AFFI) and explore its similarity to fibronectin (FN) degradation products.
(12) The active fraction, which eluted from an Affi-Gel Blue column between 0.10 and 0.15 M NaCl, was further resolved on a QMA anion exchange column.
(13) Angiotensin II antiserum purified by affinity chromatography was covalently coupled to Affi-gel 10 (Affi-gel 10-AB).
(14) However, both the GDP- and GTP-bound forms of the N-terminal fragment failed to bind to membranes and phosphatidylserine-linked Affi-Gel.
(15) Purification steps included several chromatography using Q-Sepharose Fast Flow, cellulose phosphate, Toyopearl HW 55 and Affi-Gel Blue.
(16) Dialyzed serum was chromatographed successively on Affi-gel blue, hydroxyapatite, DE-cellulose, phenyl-Sepharose, an affinity matrix for angiotensin converting enzyme and concanavalin-Sepharose.
(17) Plasminogen activating activity appeared resulting from the separation of UKI from PPA-UKI complex through UK-Sepharose or UK-Affi Gel 10 affinity chromatography.
(18) A 130-kDa integral membrane glycoprotein from brush border membrane was shown to bind specifically to 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate immobilized on Affi-Gel 102 resin.
(19) One of them was araUTP-Sepharose 4B which was coupled directly with the ligand and the other was araUTP-Affi-Gel 10 which was coupled with the ligand through a spacer.
(20) The product was purified by Affi-Gel Blue followed by Bio-Rex 70 column chromatography.
Iffy
Definition:
Example Sentences:
(1) Before you know it you can have a few iffy results.
(2) Iffy grooming habits are the least of Wolfowitz's worries as he takes on the presidency of the World Bank.
(3) If Sampson had a case for arguing that Rafferty’s inital push had been made marginally outside the area, he could have had no complaints when England – due £35,000 per woman bonuses had they lifted the trophy – won a distinctly iffy penalty of their own.
(4) The keeper has looked iffy today but letting that in would have made him absolutely whiffy.
(5) Arsenal’s players performed a cancan on the pitch at Highbury as fans chanted: “We are unbeatable.” After an iffy first half, they had won 2-1 to complete a full season without defeat in the league.
(6) It’s looking certainly more iffy than it did even just a few days ago.
(7) It's looking like an iffy pot, or a two cushion escape.
(8) Because now Nando's has been caught ploughing money into all manner of legal but morally iffy offshore tax havens, and the fragile castle of ideology that I have painstakingly constructed for myself has crumpled.
(9) When they mentioned figures, they were very iffy about whether they were positive or negative.
(10) 11.12pm BST Tweets Rory Murphy (@bogotabandit2) so @HunterFelt prediction already looking a bit iffy.
(11) Their only post-promotion victory came at Sunderland in August but, after a few slightly iffy performances, Ramírez was back to his elegant, subtly incisive, intelligent best during last Saturday’s encouraging 0-0 draw at Arsenal .
(12) Berlind's theory of ovum transmigration in the etiology of tubal pregnancy is revived and brought into line with Iffy's delayed ovulation-reflux hypothesis.
(13) In other words: Tonight is your chance to go crazy conspiracy theorists, feel free to examine the game tape surrounding every iffy call as if it were the Zapruder film.
(14) It might be one of the oldest methods of preserving fruit, but make sure you put them in an airtight container, and if you've had them for a while, now's the time to use them up – I'm a bit iffy about keeping any ingredient for longer than six months.
(15) Jarvis isn't interested in who celebrities are shagging: "People might be a bit iffy, but that's between them and their conscience.
(16) He’s the one that’s been very iffy and about his role and what he would like to do about immigration.” Reckless made the comments when he was challenged to spell out what would happen to a Polish plumber if Ukip had its way on Britain leaving the EU.
(17) Indeed, according to Jim Ward's account, only an iffy penalty decision kept Rams – Isthmian League Cup winners that season – out of the final against Wimbledon.
(18) It was a bit iffy when Garvey got to the bullying (he said "strong-willed" and "tough" too many times, like he'd spent a weekend with Miliband, brainstorming positive terms for "bastard").
(19) Everyone: Isn't it a bit iffy hanging around with her again?
(20) Asked about calls for an election pact, he said: “The internal wrangling of the Conservative party is no longer my problem … There is something a little bit iffy about the idea of pacts, because it suggests politicians fixing things for their convenience.