(1) Serological studies suggest that amaranthin does not appear to be present in the stems or leaves of the A. caudatus plant, nor were there any indications for the presence of cross-reactive material.
(2) The stoichiometry of carbohydrate binding was determined to be one T-disaccharide-binding site per amaranthin subunit (Ka = 3.6 X 10(5) M-1).
(3) In the present study we have analyzed by light and electron microscopy the distribution and subcellular localization of Amaranthin binding sites in normal, dysplastic and neoplastic colonic epithelium.
(4) In this study, neuraminidase (sialidase) and 6 different lectins, wheat germ agglutinin (WGA), Limax flavus agglutinin (LFA), Sambucus nigra agglutinin (SNA), Maackia amurensis agglutinin (MAA), peanut agglutinin (PNA), and Amaranthin, were used to histochemically characterize the carbohydrate structure of glycoconjugate in the murine eustachian tube pharyngeal orifice.
(5) A comparison between the carbohydrate-binding specificities of amaranthin and peanut (Arachis hypogaea) agglutinin is discussed.
(6) Application of the galactose oxidase-Schiff sequence abolished amaranthin (and anti-T antibody) binding to the T antigen but not to its cryptic form, and therefore permitted their differentiation in tissue sections.
(7) The lectin amaranthin, purified from the seeds of Amaranthus caudatus, has been shown to react specifically with the Gal beta 1,3GalNAc-alpha and the NeuAc alpha 2,3Gal beta 1,3GalNAc-alpha sequence which represent the T antigen and the cryptic T antigen, respectively.
(8) Therefore, amaranthin can be used for histochemical detection of the T antigen and the cryptic T antigen, and facilitates discrimination between them.
(9) Investigation of transitional mucosa, adenocarcinomas of different degrees of differentiation and mucinous carcinomas as well as adenomas with different degrees of dysplasia all revealed positive Amaranthin staining.
(10) Histochemical evidence was obtained indicating that amaranthin is a more specific anti-T reagent than peanut lectin.
(11) Amaranthin staining was inhibited by pre-incubation of lectin-gold complexes with 10 mM Gal beta 1,3GalNAc-alpha-O-benzyl (synthetic T antigen) or 10 mM Gal beta 1,3GalNAc-alpha-O-aminophenylethyl-human serum albumin (T antigen neoglycoprotein), asialoglycophorin, asialofetuin, and asialomucin.
(12) This Amaranthin staining was resistant to GO-Schiff treatment.
(13) Amaranthin is the lectin present in the seeds of Amaranthus caudatus, which specifically binds the T-disaccharide (Gal beta 1,3GalNAc alpha-O-).
(14) The lectin Amaranthin reacts with Gal beta 1,3 GalNAc-alpha (the T antigen) and NeuAc alpha 2,3 Gal beta 1,3 GalNAc-alpha (the cryptic T antigen).
(15) NeuAc alpha 2,3Gal beta 1,3GalNAc alpha-O-(CH2)8CO2CH3 was as potent an inhibitor as Gal beta 1,3GalNAc alpha-O-(CH2)8CO2-CH3, and amaranthin was precipitated by NeuAc alpha 2,3Gal beta 1,3GalNAc alpha-O-BSA (where BSA is bovine serum albumin), indicating that the amaranthin-combining site tolerates substitutions at the C'-3 hydroxyl group.
(16) A lectin (Amaranthin) present in the seeds of Amaranthus caudatus has been isolated by fractionation on DEAE-cellulose followed by affinity chromatography on Synsorb-T beads (Gal beta 1,3GalNAc alpha-O-R-Synsorb).
(17) Equilibrium sedimentation (Mr = 62,900) and low-angle laser light scattering (Mr = 61,400) methods have been used to unambiguously establish the native multimeric structure of amaranthin as a homodimer.
(18) We report here the development of labeling techniques that apply amaranthin to stain paraffin sections from rat fetuses.
(19) Studies with a homobifunctional cross-linking reagent and amaranthin further support the existence of a lectin homodimer.
(20) A native Mr = 54,000 was determined by gel filtration suggesting that amaranthin exists as a homodimer.
Colour
Definition:
(n.) See Color.
Example Sentences:
(1) A similar interference colour appeared after incubating sections of rat skin with chymase.
(2) What we’re doing is designed to improve people’s lives.” "I don't see race, colour or creed, and neither do my children," he added.
(3) They retained the ability to make this discrimination when the coloured stimuli were placed against a background bright enough to saturate the rods.3.
(4) Mendl's candy colours contrast sharply with the gothic garb of our hero's enemies and the greys of the prison uniforms – as well as scenes showing the hotel later, in the 1960s, its opulence lost beneath a drab communist refurb.
(5) On 17 December Clegg will set out his own script for the year ahead, testing the idea that coalition governments can function even as the two parties clearly show their separate colours.
(6) The Brandenburg Gate was lit up in the colours of the German flag.
(7) In his notorious 1835 Minute on Education , Lord Macaulay articulated the classic reason for teaching English, but only to a small minority of Indians: “We must do our best to form a class who may be interpreters between us and the millions whom we govern; a class of persons, Indians in blood and colour, but English in taste, in opinions, in morals and in intellect.” The language was taught to a few to serve as intermediaries between the rulers and the ruled.
(8) Bound biocytinyl-E2 is detected after binding of streptavidin-peroxidase and colour production by the enzyme.
(9) Significant biases in the distribution of cases of babesiosis were found with regard to season (P < 0,05), sex (P < 0,001) and coat colour (P < 0.01).
(10) In order to map the mental state in the early puerperium the authors gave to a group of 100 women for five days after delivery Lüscher's colour test.
(11) Trichophytosis (T. equinum) is characterized as typical numerous small and round patches, covered by small, bran-like, asbestos-coloured scales.
(12) Malvidin chloride (MC) a colouring agent from flowers of Malvaviscus conzattii Greenum was studied for male anti-fertility effects in adult langur monkeys (Presbytis entellus entellus Dufresne).
(13) The conclusion is to warn the orthopaedic surgeons to look carefully what model is behind the pretty coloured results.
(14) His bracelets and his hair, neatly gathered in a colourful elasticated band, contrast with his unflashy day-to-day uniform of checked shirts, jeans or cheap chinos and trainers.
(15) Blunt homicide predominated amongst White females, who were substantially older than the Coloured and African subjects.
(16) Variation of scrotal colour was not due to changes in melanocyte number or dispersion of melanosomes.
(17) Most striking finding was his difficulty in identifying common objects and colours along with a profound alexia.
(18) In three the diagnosis was only suspected when the colour Doppler study showed dilated intraseptal and epicardial vessels and an abnormal flow signal into the pulmonary artery in diastole; this latter signal localised the exact site of communication, which was not apparent on angiocardiography.
(19) The verbal coding and recognition of colours of a group of chronic schizophrenics and their normal controls were investigated.
(20) Scott insisted he was an abstract painter in the way he felt Chardin was too: the pans and fruit were uninteresting in themselves; they were merely "the means of making a picture", which was a study in space, form and colour.