(1) Serological studies suggest that amaranthin does not appear to be present in the stems or leaves of the A. caudatus plant, nor were there any indications for the presence of cross-reactive material.
(2) The stoichiometry of carbohydrate binding was determined to be one T-disaccharide-binding site per amaranthin subunit (Ka = 3.6 X 10(5) M-1).
(3) In the present study we have analyzed by light and electron microscopy the distribution and subcellular localization of Amaranthin binding sites in normal, dysplastic and neoplastic colonic epithelium.
(4) In this study, neuraminidase (sialidase) and 6 different lectins, wheat germ agglutinin (WGA), Limax flavus agglutinin (LFA), Sambucus nigra agglutinin (SNA), Maackia amurensis agglutinin (MAA), peanut agglutinin (PNA), and Amaranthin, were used to histochemically characterize the carbohydrate structure of glycoconjugate in the murine eustachian tube pharyngeal orifice.
(5) A comparison between the carbohydrate-binding specificities of amaranthin and peanut (Arachis hypogaea) agglutinin is discussed.
(6) Application of the galactose oxidase-Schiff sequence abolished amaranthin (and anti-T antibody) binding to the T antigen but not to its cryptic form, and therefore permitted their differentiation in tissue sections.
(7) The lectin amaranthin, purified from the seeds of Amaranthus caudatus, has been shown to react specifically with the Gal beta 1,3GalNAc-alpha and the NeuAc alpha 2,3Gal beta 1,3GalNAc-alpha sequence which represent the T antigen and the cryptic T antigen, respectively.
(8) Therefore, amaranthin can be used for histochemical detection of the T antigen and the cryptic T antigen, and facilitates discrimination between them.
(9) Investigation of transitional mucosa, adenocarcinomas of different degrees of differentiation and mucinous carcinomas as well as adenomas with different degrees of dysplasia all revealed positive Amaranthin staining.
(10) Histochemical evidence was obtained indicating that amaranthin is a more specific anti-T reagent than peanut lectin.
(11) Amaranthin staining was inhibited by pre-incubation of lectin-gold complexes with 10 mM Gal beta 1,3GalNAc-alpha-O-benzyl (synthetic T antigen) or 10 mM Gal beta 1,3GalNAc-alpha-O-aminophenylethyl-human serum albumin (T antigen neoglycoprotein), asialoglycophorin, asialofetuin, and asialomucin.
(12) This Amaranthin staining was resistant to GO-Schiff treatment.
(13) Amaranthin is the lectin present in the seeds of Amaranthus caudatus, which specifically binds the T-disaccharide (Gal beta 1,3GalNAc alpha-O-).
(14) The lectin Amaranthin reacts with Gal beta 1,3 GalNAc-alpha (the T antigen) and NeuAc alpha 2,3 Gal beta 1,3 GalNAc-alpha (the cryptic T antigen).
(15) NeuAc alpha 2,3Gal beta 1,3GalNAc alpha-O-(CH2)8CO2CH3 was as potent an inhibitor as Gal beta 1,3GalNAc alpha-O-(CH2)8CO2-CH3, and amaranthin was precipitated by NeuAc alpha 2,3Gal beta 1,3GalNAc alpha-O-BSA (where BSA is bovine serum albumin), indicating that the amaranthin-combining site tolerates substitutions at the C'-3 hydroxyl group.
(16) A lectin (Amaranthin) present in the seeds of Amaranthus caudatus has been isolated by fractionation on DEAE-cellulose followed by affinity chromatography on Synsorb-T beads (Gal beta 1,3GalNAc alpha-O-R-Synsorb).
(17) Equilibrium sedimentation (Mr = 62,900) and low-angle laser light scattering (Mr = 61,400) methods have been used to unambiguously establish the native multimeric structure of amaranthin as a homodimer.
(18) We report here the development of labeling techniques that apply amaranthin to stain paraffin sections from rat fetuses.
(19) Studies with a homobifunctional cross-linking reagent and amaranthin further support the existence of a lectin homodimer.
(20) A native Mr = 54,000 was determined by gel filtration suggesting that amaranthin exists as a homodimer.
Eternal
Definition:
(a.) Without beginning or end of existence; always existing.
(a.) Without end of existence or duration; everlasting; endless; immortal.
(a.) Continued without intermission; perpetual; ceaseless; constant.
(a.) Existing at all times without change; immutable.
(a.) Exceedingly great or bad; -- used as a strong intensive.
(n.) One of the appellations of God.
(n.) That which is endless and immortal.
Example Sentences:
(1) Will the United fans' eternal favourite soon add his voice to that of 140,000 fans?
(2) The lucky thing is, says Susan Calman , that although she is "an eternal worrier, occasionally I do something stupid."
(3) In legend, Gilgamesh fell asleep on the water side and let slip from his fingers the plant of eternal youth.
(4) To butcher TS Eliot: I have seen the mercury of my thermometer flicker, And I have seen the eternal footman hold my sheets drenched in sweat at 3am, and snicker, And in short, I was too hot.
(5) Dayton Flyers once again pull off the round's first upset The final minute of game time seemed to take a small eternity in real time, with the in-game action interrupted by four team timeouts and eight free throw attempts.
(6) Greed is not only good, it is a fundamental prop to the fantasy of eternal growth.
(7) In each of his creative capacities, he was the eternal quiet man.
(8) Even Alec – eternally hard to please where his own work was concerned – loved it.
(9) 9.06am BST There are some eternal verities in politics and one of them is that British governments (especially Conservative-led ones) are always fighting a war on red tape.
(10) Boris Johnson accused of 'dishonest gymnastics' over TTIP U-turn Read more “But fundamentally, what is lacking is the eternal problem, which is that there is no underlying loyalty to the idea of Europe .
(11) They call it, rather unsurprisingly, the eternal flame.
(12) They were created on the basis that, whatever the cost, there are some eternal values that are worth upholding in a civilised society.
(13) He has taken the legacy of postwar abstract expressionism, and allied to that a deep love of the great eternal themes of the classical world.
(14) Murray said: "I'm eternally grateful to Ivan for all his hard work over the past two years, the most successful of my career so far.
(15) I am not sure that a lucrative career in rape gags is more helpful than a failed one, but the rape hum seems eternal.
(16) Ras proteins are membrane-associated transducers of eternal stimuli to unknown intracellular targets.
(17) One, Baroness O'Cathain, has said, in relation to politics and her evangelism: "For me it is a guarantee of eternal peace."
(18) Committed to eliminating the budget deficit by the end of next year, it just does not have the cash to fund, for example, big new infrastructure projects like an eternally proposed (and eternally postponed) bridge over the Straits of Messina.
(19) François Bayrou must have resigned himself to being the eternal also-ran of French presidential elections, by now.
(20) To all those who offered me their friendship, support and prayers, I will be eternally grateful.