(1) We used the polymerase chain reaction (PCR) to amplify the breakpoint area of alpha-thalassemia-1 of Southeast Asia type and several parts of the alpha-globin gene cluster to make a differential diagnosis between alpha-thalassemia-1 and Hb Bart's hydrops fetalis.
(2) We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
(3) We conclude that only one of the alleles was amplified and that either allele could be amplified with respect to both the SphI and PvuII polymorphisms.
(4) RNA preparations were reverse-transcribed, amplified by a polymerase chain reaction (PCR), and separated by electrophoresis.
(5) It was previously demonstrated that multidrug-resistant Chinese hamster cell lines contain an amplified, transcriptionally active DNA sequence designated mdr.
(6) Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) (iii) The insertion plasmid pRSD102(R388::Tn1721) has conserved the capacity of the original plasmid pRSD1 to amplify the 3.55 Mdal-tet region.
(9) To identify the molecular defect in a patient with the intermediate form of the disease, arylsulfatase B mRNA from his fibroblasts was reverse-transcribed, amplified by the polymerase chain reaction, and subcloned.
(10) In addition to the fatigue tester and the pulse duplicator, a signal conditioner, a DC amplifier, an analog-to-digital converter, and a digital microcomputer comprised the essential hardware.
(11) The sensitivity of a PCR system to amplify the long terminal repeat (LTR) sequence of HIV-1 was not affected by the irradiation procedure; however, the ultimate sensitivity of a PCR system for the amplification of an early gene promotor sequence of the CMV genome was reduced 1000-fold.
(12) Thus MTX combined with PALA selected cells with an 'amplificator' phenotype (an increased ability to amplify parts of the genome).
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) Whether we would use that to support and amplify the community ideals already present or go the way of gentrification remained to be seen.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) The action of exogenous etiologic agents is usually mediated in part or amplified by endogenous mediators that very often exert biphasic, i.e., damaging and protective, effects.
(19) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
(20) The PCR amplified a 375-bp DNA fragment which was cloned and sequenced; the deduced amino acid sequence had significant identity with known TS sequences, including strict conservation of all phylogenetically invariant TS amino acid residues.
Amplitude
Definition:
(n.) State of being ample; extent of surface or space; largeness of dimensions; size.
(n.) Largeness, in a figurative sense; breadth; abundance; fullness.
(n.) Of extent of capacity or intellectual powers.
(n.) Of extent of means or resources.
(n.) The arc of the horizon between the true east or west point and the center of the sun, or a star, at its rising or setting. At the rising, the amplitude is eastern or ortive: at the setting, it is western, occiduous, or occasive. It is also northern or southern, when north or south of the equator.
(n.) The arc of the horizon between the true east or west point and the foot of the vertical circle passing through any star or object.
(n.) The horizontal line which measures the distance to which a projectile is thrown; the range.
(n.) The extent of a movement measured from the starting point or position of equilibrium; -- applied especially to vibratory movements.
(n.) An angle upon which the value of some function depends; -- a term used more especially in connection with elliptic functions.
Example Sentences:
(1) After midazolam infusion, there was a 50% decrease in amplitude of P3 in response to target tones (P less than 0.006), whereas N3 latency increased by 40 ms (P less than 0.05).
(2) A significant correlation was found between the amplitude ratio of the R2 and the sensitivity ratio of the rapid off-response at short and long wavelengths.
(3) Intact rams exhibited GH secretory episodes of greater (P less than 0.01) amplitude than did castrated lambs.
(4) The maximum amplitude of the inward Na+ current, normalized by cell capacitance, is about sixfold larger, on the average, in LP lactotropes than in SP lactotropes.
(5) The examination of the standard waves' amplitude and latency of the brain stem auditory evoked response (BAEP) was performed in 20 guinea pigs (males and females, weighing 250 to 300 g).
(6) The amplitudes of the a-wave and the 01 decreased in dose-dependent manners, but their changes were less striking than those of the 01 latency.
(7) We have now started a prospective follow-up study in order to pursue the development of (a) p-ERG amplitudes and (b) funduscopic changes and visual acuity in these patients.
(8) Noradrenaline decreased the phasic contraction amplitude of the circular muscle and exerted a stimulant effect on the tone which suggested an existence of two alpha 1-adrenoceptor subtypes.
(9) elution patterns of the adducts formed by DBF metabolites with DNA and obtained in vivo at the optimal exposure time of 42-48 h were qualitatively very similar to the patterns obtained in vitro, but their amplitude was quantitatively reduced.
(10) In contrast, the average reduction in mean EEG amplitude with isoflurane was only 0.3% and there were neither periods of suppression nor any correlation between EEG amplitude and MAP.
(11) However, the effect of prior jaw motion and the effect of the recording site on the EMG amplitudes and on the vertical dimension of minimum EMG activity have not been documented.
(12) The ophthalmic headache's crisis is caused, in fact, by a spasm of convergence on an unknown exophory of which the amplitude of fusion is satisfying, and the presence of which can only be seen with test under screen.
(13) Moreover, the most recent combined application of the rat interstitial cell testosterone (RICT) bioassay and a novel multiple-parameter deonvolution model has allowed investigators to dissect plasma concentration profiles of bioactive LH into defined secretory bursts, which have numerically explicit amplitudes, locations in time, and durations, and are acted upon by determinable subject- and study-specific endogenous metabolic clearance rates.
(14) By this action, oxytocin is believed to increase the probability of successful regenerative spikes and thereby initiate electrical activity in quiescent preparations, increase the frequency of burst discharges, the number of spikes in each burst, and the amplitude of spikes in individual cells.
(15) Replication dependent on the SV40 origin and having the kinetics and approximate amplitude of an SV40 infection ensued.
(16) For the case of the fluctuating pressure, the strength of the artery becomes considerably lower than those under constant amplitude and two-step-multi-duplicated pulsatile pressure.
(17) Complex tones containing the first 20 harmonics of 50, 100, or 200 Hz, all at equal amplitude, were used.
(18) In the placebo group of 14 patients CNV-amplitudes even decreased after ten Infusions with multi-vitamins alone.
(19) ERGs of high amplitude and of normal wave form were recordable with prominent oscillatory potentials.
(20) increase in amplitude in both, hippocampus and neocortex was observed with MK 801 and ketamine and these agents also showed longer lasting influence.