(1) We used the polymerase chain reaction (PCR) to amplify the breakpoint area of alpha-thalassemia-1 of Southeast Asia type and several parts of the alpha-globin gene cluster to make a differential diagnosis between alpha-thalassemia-1 and Hb Bart's hydrops fetalis.
(2) We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
(3) We conclude that only one of the alleles was amplified and that either allele could be amplified with respect to both the SphI and PvuII polymorphisms.
(4) RNA preparations were reverse-transcribed, amplified by a polymerase chain reaction (PCR), and separated by electrophoresis.
(5) It was previously demonstrated that multidrug-resistant Chinese hamster cell lines contain an amplified, transcriptionally active DNA sequence designated mdr.
(6) Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) (iii) The insertion plasmid pRSD102(R388::Tn1721) has conserved the capacity of the original plasmid pRSD1 to amplify the 3.55 Mdal-tet region.
(9) To identify the molecular defect in a patient with the intermediate form of the disease, arylsulfatase B mRNA from his fibroblasts was reverse-transcribed, amplified by the polymerase chain reaction, and subcloned.
(10) In addition to the fatigue tester and the pulse duplicator, a signal conditioner, a DC amplifier, an analog-to-digital converter, and a digital microcomputer comprised the essential hardware.
(11) The sensitivity of a PCR system to amplify the long terminal repeat (LTR) sequence of HIV-1 was not affected by the irradiation procedure; however, the ultimate sensitivity of a PCR system for the amplification of an early gene promotor sequence of the CMV genome was reduced 1000-fold.
(12) Thus MTX combined with PALA selected cells with an 'amplificator' phenotype (an increased ability to amplify parts of the genome).
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) Whether we would use that to support and amplify the community ideals already present or go the way of gentrification remained to be seen.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) The action of exogenous etiologic agents is usually mediated in part or amplified by endogenous mediators that very often exert biphasic, i.e., damaging and protective, effects.
(19) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
(20) The PCR amplified a 375-bp DNA fragment which was cloned and sequenced; the deduced amino acid sequence had significant identity with known TS sequences, including strict conservation of all phylogenetically invariant TS amino acid residues.
Appliance
Definition:
(n.) The act of applying; application; [Obs.] subservience.
(n.) The thing applied or used as a means to an end; an apparatus or device; as, to use various appliances; a mechanical appliance; a machine with its appliances.
Example Sentences:
(1) This article examines the history of Dr. Crozat and his appliance, discusses the development and divergence in its use, and demonstrates this divergence with a few selected, documented case reports.
(2) It is not same to the stainless steel wire of traditional removable appliances which must be activated every time to produce a little tooth movement.
(3) Following orthodontic treatment the canine's incisal edge occlusion demonstrates the tip and torque present in the appliance that was used.
(4) The effects of maxillary protracting bow appliance were the maxillary forward movement associated with counter-clockwise rotation of the nasal floor and the mandibular backward movement associated with clockwise rotation.
(5) Adjustment of posterior arch width and dental alignment, using semi-rapid maxillary expansion by means of an upper removable appliance, to co-ordinate the anticipated positions for the arches.
(6) There was a significant increase in gingival index at day 21 in areas where the appliance covered the gingival margin.
(7) The appliance provides the orthodontist with an extensive range of options in treatment mechanics--from anchorage conservation and rapid movement of limited tipping by light forces to translation or stabilization with precise three-dimensional control.
(8) An infant with a complete unilateral cleft of the lip and palate underwent maxillary expansion treatment using an oral orthopedic appliance.
(9) Six individuals wore the appliances while rinsing daily with a neutral 0.2% NaF solution for 4 wk.
(10) Although prostheses are not anatomical avatars, careful appliance prescription and training, coordinated with the child's growth and developmental changes, can optimize the benefits the child derives from the prosthesis.
(11) Orthodontic closure of the space from both sides was performed with fixed appliance, leaving the remaining central incisor in the midline.
(12) Far too frequently, however, the clinician limits his appliance design to a selective number of appliances with which he is conversant and attempts to modify any one of them to fit a specific situation.
(13) The treatment effects of continuous bite jumping with the Herbst appliance in the correction of Class II malocclusions have been analysed in previous investigations.
(14) Conservative treatment consists of exercises and shoe appliances.
(15) Experience from the use of feeding plates for babies with cleft palate and from the treatment of dysphagia in patients recovering from stroke led to the design of a simple intraoral appliance.
(16) Three cases of asphyxial deaths as a result of aspiration of dental appliances are presented.
(17) Although the muscles of untreated children also showed shifts of mean frequency to lower frequency values as a function of time, there was a greater downward shift of mean frequency in those treated with functional appliances.
(18) Annual savings in tonnes of CO 2 Install 2 kilowatt solar PV panels 0.4 Buy a new A++ refrigerator if yours is more than 4 years old, and only use a small-screen TV 0.1 Use LED or fluorescent lights where you currently have halogen lights installed 0.1 Buy an automated system to turn off appliances when not in use; get a meter that shows actual energy use and use it to monitor your household 0.1 Only use your washing machine and dishwasher when full to capacity and at lowest temperature 0.1 Never use the tumble dryer 0.1 Get rid of the freezer if you can, and replace your small appliances with "eco" varieties 0.1 Car (1.5 tonnes of CO 2 ) There is one car for every two people in the UK, and each one travels an average of about 9,000 miles a year.
(19) The mandible does tend to rotate in a counterclockwise manner following enucleation of four first premolars without appliance therapy.
(20) The purpose of the study was to analyse and compare deep overbite correction in adult patients carried out by a straight wire appliance and the segmented arch technique as recommended by Burstone.