(1) We used the polymerase chain reaction (PCR) to amplify the breakpoint area of alpha-thalassemia-1 of Southeast Asia type and several parts of the alpha-globin gene cluster to make a differential diagnosis between alpha-thalassemia-1 and Hb Bart's hydrops fetalis.
(2) We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
(3) We conclude that only one of the alleles was amplified and that either allele could be amplified with respect to both the SphI and PvuII polymorphisms.
(4) RNA preparations were reverse-transcribed, amplified by a polymerase chain reaction (PCR), and separated by electrophoresis.
(5) It was previously demonstrated that multidrug-resistant Chinese hamster cell lines contain an amplified, transcriptionally active DNA sequence designated mdr.
(6) Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) (iii) The insertion plasmid pRSD102(R388::Tn1721) has conserved the capacity of the original plasmid pRSD1 to amplify the 3.55 Mdal-tet region.
(9) To identify the molecular defect in a patient with the intermediate form of the disease, arylsulfatase B mRNA from his fibroblasts was reverse-transcribed, amplified by the polymerase chain reaction, and subcloned.
(10) In addition to the fatigue tester and the pulse duplicator, a signal conditioner, a DC amplifier, an analog-to-digital converter, and a digital microcomputer comprised the essential hardware.
(11) The sensitivity of a PCR system to amplify the long terminal repeat (LTR) sequence of HIV-1 was not affected by the irradiation procedure; however, the ultimate sensitivity of a PCR system for the amplification of an early gene promotor sequence of the CMV genome was reduced 1000-fold.
(12) Thus MTX combined with PALA selected cells with an 'amplificator' phenotype (an increased ability to amplify parts of the genome).
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) Whether we would use that to support and amplify the community ideals already present or go the way of gentrification remained to be seen.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) The action of exogenous etiologic agents is usually mediated in part or amplified by endogenous mediators that very often exert biphasic, i.e., damaging and protective, effects.
(19) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
(20) The PCR amplified a 375-bp DNA fragment which was cloned and sequenced; the deduced amino acid sequence had significant identity with known TS sequences, including strict conservation of all phylogenetically invariant TS amino acid residues.
Circuit
Definition:
(n.) The act of moving or revolving around, or as in a circle or orbit; a revolution; as, the periodical circuit of the earth round the sun.
(n.) The circumference of, or distance round, any space; the measure of a line round an area.
(n.) That which encircles anything, as a ring or crown.
(n.) The space inclosed within a circle, or within limits.
(n.) A regular or appointed journeying from place to place in the exercise of one's calling, as of a judge, or a preacher.
(n.) A certain division of a state or country, established by law for a judge or judges to visit, for the administration of justice.
(n.) A district in which an itinerant preacher labors.
(n.) Circumlocution.
(v. i.) To move in a circle; to go round; to circulate.
(v. t.) To travel around.
Example Sentences:
(1) Circuit weight training does not exacerbate resting or exercise blood pressure and may have beneficial effects.
(2) Hypertrophy is restricted to subdivisions of the inferior olive included in recurrent cerebello-mesencephalic-olivary circuits.
(3) The ability of autoregulate blood flow in the extracorporeal membrane oxygenation (ECMO) circuit is critical to prevent cavitation and air embolism.
(4) To explain some of these results a theoretical model is presented to demonstrate that while short circuiting can block the passive ionic movement, it will cause an increase in the energy consumption of the system and introduce certain important changes in the ionic barriers and e.m.fs.
(5) DNase I microspheres were then introduced into the extracorporeal circuit which resulted in an acceleration of degradation of acid precipitable 125I-nDNA.
(6) A wide range of development possibilities for the printed circuit microelectrode are discussed.
(7) Our results were consistent with the modern anesthesia standard in closed circuit t.i.
(8) One hour after terminating the extacorporeal circuit, the C.O.P.
(9) These effects are not accompanied by significant changes in the transmural electrical potential difference or short-circuit current.
(10) Evidence is reviewed suggesting that this latter system may involve a corticostriatal circuit.
(11) Several attempts at circuit interruption of type 1 atrial flutter by means of surgical or catheter techniques have been published.
(12) To eliminate pacing stimulus afterpotential and detect an evoked response, a hardware feedback circuit and a software template matching algorithm were used to produce a triphasic charge-balanced pacing pulse.
(13) Four blood filters included in the extracorporeal circuit were removed one by one at 30-minute intervals.
(14) In the ECMO patient, cardiac stun syndrome and electromechanical dissociation can be confused with low circuit volume, pneumothorax, or cardiac tamponade.
(15) The transport system was analyzed in terms of an equivalent circuit model comprising a proton motive force (PMF), an active conductance (LH) in series with the pump, and a parallel or passive conductance which may be ignored in this preparation.
(16) The type 3 pattern occurred when the antidromic wavefront of early premature beats captured the original circuit exit.
(17) Polymethacrylate coated charcoal was inserted in the dialysis circuit before the dialyzer.
(18) Since our system is adjusted with square waveforms and composed of a simple analog circuit, it can be compensated easily in real time.
(19) The circuit training exercise program, therefore, appears to be an effective method for improving the fitness level of alcoholic patients.
(20) Thus, neurons of the habenula and interpeduncular nucleus are under the direct and indirect influence of septal neurons within the limbic forebrain circuit.