(1) We used the polymerase chain reaction (PCR) to amplify the breakpoint area of alpha-thalassemia-1 of Southeast Asia type and several parts of the alpha-globin gene cluster to make a differential diagnosis between alpha-thalassemia-1 and Hb Bart's hydrops fetalis.
(2) We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
(3) We conclude that only one of the alleles was amplified and that either allele could be amplified with respect to both the SphI and PvuII polymorphisms.
(4) RNA preparations were reverse-transcribed, amplified by a polymerase chain reaction (PCR), and separated by electrophoresis.
(5) It was previously demonstrated that multidrug-resistant Chinese hamster cell lines contain an amplified, transcriptionally active DNA sequence designated mdr.
(6) Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) (iii) The insertion plasmid pRSD102(R388::Tn1721) has conserved the capacity of the original plasmid pRSD1 to amplify the 3.55 Mdal-tet region.
(9) To identify the molecular defect in a patient with the intermediate form of the disease, arylsulfatase B mRNA from his fibroblasts was reverse-transcribed, amplified by the polymerase chain reaction, and subcloned.
(10) In addition to the fatigue tester and the pulse duplicator, a signal conditioner, a DC amplifier, an analog-to-digital converter, and a digital microcomputer comprised the essential hardware.
(11) The sensitivity of a PCR system to amplify the long terminal repeat (LTR) sequence of HIV-1 was not affected by the irradiation procedure; however, the ultimate sensitivity of a PCR system for the amplification of an early gene promotor sequence of the CMV genome was reduced 1000-fold.
(12) Thus MTX combined with PALA selected cells with an 'amplificator' phenotype (an increased ability to amplify parts of the genome).
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) Whether we would use that to support and amplify the community ideals already present or go the way of gentrification remained to be seen.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) The action of exogenous etiologic agents is usually mediated in part or amplified by endogenous mediators that very often exert biphasic, i.e., damaging and protective, effects.
(19) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
(20) The PCR amplified a 375-bp DNA fragment which was cloned and sequenced; the deduced amino acid sequence had significant identity with known TS sequences, including strict conservation of all phylogenetically invariant TS amino acid residues.
Course
Definition:
(n.) The act of moving from one point to another; progress; passage.
(n.) The ground or path traversed; track; way.
(n.) Motion, considered as to its general or resultant direction or to its goal; line progress or advance.
(n.) Progress from point to point without change of direction; any part of a progress from one place to another, which is in a straight line, or on one direction; as, a ship in a long voyage makes many courses; a course measured by a surveyor between two stations; also, a progress without interruption or rest; a heat; as, one course of a race.
(n.) Motion considered with reference to manner; or derly progress; procedure in a certain line of thought or action; as, the course of an argument.
(n.) Customary or established sequence of events; recurrence of events according to natural laws.
(n.) Method of procedure; manner or way of conducting; conduct; behavior.
(n.) A series of motions or acts arranged in order; a succession of acts or practices connectedly followed; as, a course of medicine; a course of lectures on chemistry.
(n.) The succession of one to another in office or duty; order; turn.
(n.) That part of a meal served at one time, with its accompaniments.
(n.) A continuous level range of brick or stones of the same height throughout the face or faces of a building.
(n.) The lowest sail on any mast of a square-rigged vessel; as, the fore course, main course, etc.
(n.) The menses.
(v. t.) To run, hunt, or chase after; to follow hard upon; to pursue.
(v. t.) To cause to chase after or pursue game; as, to course greyhounds after deer.
(v. t.) To run through or over.
(v. i.) To run as in a race, or in hunting; to pursue the sport of coursing; as, the sportsmen coursed over the flats of Lancashire.
(v. i.) To move with speed; to race; as, the blood courses through the veins.
Example Sentences:
(1) This particular variant of rheumatoid arthritis (RA) is characterized by the presence of subcutaneous rheumatoid nodules, scanty or absent systemic manifestations and a clinically benign course.
(2) A 2.5-month-old child with cyanotic heart disease who required long-term PGE1 infusions; developed widespread periosteal reactions during the course of therapy.
(3) These included bringing in the A* grade, reducing the number of modules from six to four, and a greater attempt to assess the whole course at the end.
(4) Therefore, it is suggested that PE patients without endogenous erythroid colonies may follow almost the same clinical course as SP patients.
(5) Twenty-seven patients were randomized to receive either 50 mg stanozolol or placebo intramuscularly 24 h before operation, followed by a 6 week course of either 5 mg stanozolol or placebo orally, twice daily.
(6) It is followed by rapid neurobehavioral deterioration in late infancy or early childhood, a developmental arrest, plateauing, and then either a course of retarded development or continued deterioration.
(7) In dorsoventral (DV) reversed wings at both shoulder or flank level, the motor axons do not alter their course as they enter the graft.
(8) The program met with continued support and enthusiasm from nurse administrators, nursing unit managers, clinical educators, ward staff and course participants.
(9) Of course the job is not done and we will continue to remain vigilant to all risks, particularly when the global economic situation is so uncertain,” the chancellor said in a statement.
(10) low molecular weight dextran in the course of right heart catheterization.
(11) Community involvement is a key element of the Primary Health Care (PHC) approach, and thus an essential topic on a course for managers of Primary Health Care programmes.
(12) The time-course and dose-response for this modification of pp60c-src paralleled PDGF-induced increases in phosphorylation of pp36, a major cellular substrate for several tyrosine-specific protein kinases.
(13) The evidence suggests that by the age of 15 years many adolescents show a reliable level of competence in metacognitive understanding of decision-making, creative problem-solving, correctness of choice, and commitment to a course of action.
(14) The course of urogenital tuberculosis is complicated by unspecific bacterial infections of the urinary tract and nephrolithiasis.
(15) In the course of the syndrome development blood vessel permeability was increased in the anterior chamber of the eye.
(16) Mieko Nagaoka took just under an hour and 16 minutes to finish the race as the sole competitor in the 100 to 104-year-old category at a short course pool in Ehime, western Japan , on Saturday.
(17) The time course of the current potentiation was similar to that seen with beta-adrenergic stimulation.
(18) Such complications as intracerebral haematoma or meningeal haemorrhage may occur during the usually benign course of the disease.
(19) Several dimensions of the outcome of 86 schizophrenic patients were recorded 1 year after discharge from inpatient index-treatment to complete a prospective study concerning the course of illness (rehospitalization, symptoms, employment and social contacts).
(20) The course was further complicated by administration of gentamicin, an antibiotic known to potentiate neuromuscular blocking drugs.