(1) We used the polymerase chain reaction (PCR) to amplify the breakpoint area of alpha-thalassemia-1 of Southeast Asia type and several parts of the alpha-globin gene cluster to make a differential diagnosis between alpha-thalassemia-1 and Hb Bart's hydrops fetalis.
(2) We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
(3) We conclude that only one of the alleles was amplified and that either allele could be amplified with respect to both the SphI and PvuII polymorphisms.
(4) RNA preparations were reverse-transcribed, amplified by a polymerase chain reaction (PCR), and separated by electrophoresis.
(5) It was previously demonstrated that multidrug-resistant Chinese hamster cell lines contain an amplified, transcriptionally active DNA sequence designated mdr.
(6) Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) (iii) The insertion plasmid pRSD102(R388::Tn1721) has conserved the capacity of the original plasmid pRSD1 to amplify the 3.55 Mdal-tet region.
(9) To identify the molecular defect in a patient with the intermediate form of the disease, arylsulfatase B mRNA from his fibroblasts was reverse-transcribed, amplified by the polymerase chain reaction, and subcloned.
(10) In addition to the fatigue tester and the pulse duplicator, a signal conditioner, a DC amplifier, an analog-to-digital converter, and a digital microcomputer comprised the essential hardware.
(11) The sensitivity of a PCR system to amplify the long terminal repeat (LTR) sequence of HIV-1 was not affected by the irradiation procedure; however, the ultimate sensitivity of a PCR system for the amplification of an early gene promotor sequence of the CMV genome was reduced 1000-fold.
(12) Thus MTX combined with PALA selected cells with an 'amplificator' phenotype (an increased ability to amplify parts of the genome).
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) Whether we would use that to support and amplify the community ideals already present or go the way of gentrification remained to be seen.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) The action of exogenous etiologic agents is usually mediated in part or amplified by endogenous mediators that very often exert biphasic, i.e., damaging and protective, effects.
(19) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
(20) The PCR amplified a 375-bp DNA fragment which was cloned and sequenced; the deduced amino acid sequence had significant identity with known TS sequences, including strict conservation of all phylogenetically invariant TS amino acid residues.
Order
Definition:
(n.) Regular arrangement; any methodical or established succession or harmonious relation; method; system
(n.) Of material things, like the books in a library.
(n.) Of intellectual notions or ideas, like the topics of a discource.
(n.) Of periods of time or occurrences, and the like.
(n.) Right arrangement; a normal, correct, or fit condition; as, the house is in order; the machinery is out of order.
(n.) The customary mode of procedure; established system, as in the conduct of debates or the transaction of business; usage; custom; fashion.
(n.) Conformity with law or decorum; freedom from disturbance; general tranquillity; public quiet; as, to preserve order in a community or an assembly.
(n.) That which prescribes a method of procedure; a rule or regulation made by competent authority; as, the rules and orders of the senate.
(n.) A command; a mandate; a precept; a direction.
(n.) Hence: A commission to purchase, sell, or supply goods; a direction, in writing, to pay money, to furnish supplies, to admit to a building, a place of entertainment, or the like; as, orders for blankets are large.
(n.) A number of things or persons arranged in a fixed or suitable place, or relative position; a rank; a row; a grade; especially, a rank or class in society; a group or division of men in the same social or other position; also, a distinct character, kind, or sort; as, the higher or lower orders of society; talent of a high order.
(n.) A body of persons having some common honorary distinction or rule of obligation; esp., a body of religious persons or aggregate of convents living under a common rule; as, the Order of the Bath; the Franciscan order.
(n.) An ecclesiastical grade or rank, as of deacon, priest, or bishop; the office of the Christian ministry; -- often used in the plural; as, to take orders, or to take holy orders, that is, to enter some grade of the ministry.
(n.) The disposition of a column and its component parts, and of the entablature resting upon it, in classical architecture; hence (as the column and entablature are the characteristic features of classical architecture) a style or manner of architectural designing.
(n.) An assemblage of genera having certain important characters in common; as, the Carnivora and Insectivora are orders of Mammalia.
(n.) The placing of words and members in a sentence in such a manner as to contribute to force and beauty or clearness of expression.
(n.) Rank; degree; thus, the order of a curve or surface is the same as the degree of its equation.
(n.) To put in order; to reduce to a methodical arrangement; to arrange in a series, or with reference to an end. Hence, to regulate; to dispose; to direct; to rule.
(n.) To give an order to; to command; as, to order troops to advance.
(n.) To give an order for; to secure by an order; as, to order a carriage; to order groceries.
(n.) To admit to holy orders; to ordain; to receive into the ranks of the ministry.
(v. i.) To give orders; to issue commands.
Example Sentences:
(1) The predicted non-Lorentzian line shapes and widths were found to be in good agreement with experimental results, indicating that the local orientational order (called "packing" by many workers) in the bilayers of small vesicles and in multilamellar membranes is substantially the same.
(2) "As the investigation remains live and in order to preserve the integrity of that investigation, it would not be appropriate to offer further comment."
(3) The various evocational changes appear to form sets of interconnected systems and this complex network seems to embody some plasticity since it has been possible to suppress experimentally some of the most universal evocational events or alter their temporal order without impairing evocation itself.
(4) Treatment termination due to lack of efficacy or combined insufficient therapeutic response and toxicity proved to be influenced by the initial disease activity and by the rank order of prescription.
(5) The LD50 of the following metal-binding chelating drugs, EDTA, diethylenetriaminepentaacetic acid (DTPA), hydroxyethylenediaminetriacetic acid (HEDTA), cyclohexanediaminotetraacetic acid (CDTA) and triethylenetetraminehexaacetic acid (TTHA) was evaluated in terms of mortality in rats after intraperitoneal administration and was found to be in the order: CDTA greater than EDTA greater than DTPA greater than TTHA greater than HEDTA.
(6) Squadron Leader Kevin Harris, commander of the Merlins at Camp Bastion, the main British base in Helmand, praised the crews, adding: "The Merlins will undergo an extensive programme of maintenance and cleaning before being packed up, ensuring they return to the UK in good order."
(7) In order to control noise- and vibration-caused diseases it was necessary not only to improve machines' quality and service conditions but also to pay special attention to the choice of operators and to the quality of monitoring their adaptation process.
(8) The purpose of this paper is to discuss the potential for integrating surveillance techniques in reproductive epidemiology with geographic information system technology in order to identify populations at risk around hazardous waste sites.
(9) In order to determine the extent of this similarity, I have developed a panel of probes for many of the Pacl restriction fragments and have shown that most of the Pacl and Notl fragments found in MBa are also present in MBb.
(10) In addition to the phase diagrams reported here for these two binary mixtures, a brief theoretical discussion is given of other possible phase diagrams that may be appropriate to other lipid mixtures with particular consideration given to the problem of crystalline phases of different structures and the possible occurrence of second-order phase transitions in these mixtures.
(11) However, within 5 min potassium overcame the vanadate potentiation of ouabain binding regardless of the order in which it was added to the reaction mixture.
(12) The metabolism of [1,3-14C]benzo[f]quinoline (BfQ) by liver microsomes from control, 3-methylcholanthrene (3-MC)-pretreated and phenobarbital (PB)-pretreated rats has been investigated in order to gain insights into the effect of mixed function oxidase inducers on the types and levels of specific metabolites as formed in vitro.
(13) Friend erythroleukemia cells were induced to differentiate by dimethyl sulfoxide (DMSO) and hexamethylene-bis-acetamide (HBMA) in order to investigate whether their lipid characteristics, common to other systems of transformed cells, revert to a normal differentiation pattern.
(14) In order for the club to grow and sustain its ability to be a competitive force in the Premier League, the board has made a number of decisions which will strengthen the club, support the executive team, manager and his staff and enhance shareholder return.
(15) The present study was done in order to document the ability of the eighth cranial nerve of the bullfrog (Rana catesbeiana) to regenerate, the anatomic characteristics of the regenerated fibers, and the specificity of projections from individual endorgan branches of the nerve.
(16) In order to develop a sampling strategy and a method for analyzing the circadian body temperature pattern, we monitored estimates of the temperature in four ways using rectal, oral, axillary and deep body temperature from the skin surface every hour for 72 consecutive hours in 10 normal control subjects.
(17) I fear that I will have to go through another witch-hunt in order to apply for this benefit."
(18) A retrospective study was done in 86 patients on dialysis in order to evaluate the doses of aluminum hydroxide (OH3 Al) received to achieve a better serum phosphate control.
(19) Each test was examined by the frequency with which it was ordered, the frequency with which it was abnormal, and the frequency with which the abnormal result affected preoperative care.
(20) We have now started a prospective follow-up study in order to pursue the development of (a) p-ERG amplitudes and (b) funduscopic changes and visual acuity in these patients.