(1) We used the polymerase chain reaction (PCR) to amplify the breakpoint area of alpha-thalassemia-1 of Southeast Asia type and several parts of the alpha-globin gene cluster to make a differential diagnosis between alpha-thalassemia-1 and Hb Bart's hydrops fetalis.
(2) We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
(3) We conclude that only one of the alleles was amplified and that either allele could be amplified with respect to both the SphI and PvuII polymorphisms.
(4) RNA preparations were reverse-transcribed, amplified by a polymerase chain reaction (PCR), and separated by electrophoresis.
(5) It was previously demonstrated that multidrug-resistant Chinese hamster cell lines contain an amplified, transcriptionally active DNA sequence designated mdr.
(6) Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) (iii) The insertion plasmid pRSD102(R388::Tn1721) has conserved the capacity of the original plasmid pRSD1 to amplify the 3.55 Mdal-tet region.
(9) To identify the molecular defect in a patient with the intermediate form of the disease, arylsulfatase B mRNA from his fibroblasts was reverse-transcribed, amplified by the polymerase chain reaction, and subcloned.
(10) In addition to the fatigue tester and the pulse duplicator, a signal conditioner, a DC amplifier, an analog-to-digital converter, and a digital microcomputer comprised the essential hardware.
(11) The sensitivity of a PCR system to amplify the long terminal repeat (LTR) sequence of HIV-1 was not affected by the irradiation procedure; however, the ultimate sensitivity of a PCR system for the amplification of an early gene promotor sequence of the CMV genome was reduced 1000-fold.
(12) Thus MTX combined with PALA selected cells with an 'amplificator' phenotype (an increased ability to amplify parts of the genome).
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) Whether we would use that to support and amplify the community ideals already present or go the way of gentrification remained to be seen.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) The action of exogenous etiologic agents is usually mediated in part or amplified by endogenous mediators that very often exert biphasic, i.e., damaging and protective, effects.
(19) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
(20) The PCR amplified a 375-bp DNA fragment which was cloned and sequenced; the deduced amino acid sequence had significant identity with known TS sequences, including strict conservation of all phylogenetically invariant TS amino acid residues.
Speaker
Definition:
(n.) One who speaks.
(n.) One who utters or pronounces a discourse; usually, one who utters a speech in public; as, the man is a good speaker, or a bad speaker.
(n.) One who is the mouthpiece of others; especially, one who presides over, or speaks for, a delibrative assembly, preserving order and regulating the debates; as, the Speaker of the House of Commons, originally, the mouthpiece of the House to address the king; the Speaker of a House of Representatives.
(n.) A book of selections for declamation.
Example Sentences:
(1) Significant differences between laryngectomee and nonlaryngectomee judges were found when rating alaryngeal speakers, but not when rating normal, laryngeal speakers.
(2) In the experiments to be reported here, computer-averaged EMG data were obtained from PCA of native speakers of American English, Japanese, and Danish who uttered test words embedded in frame sentences.
(3) The speaker issued his warning after William Hague told MPs that the government would consult parliament but declined to explain the nature of the vote.
(4) The present study examines kinematic details of the laryngeal articulatory gesture in 2 deaf speakers and a control subject using transillumination of the larynx.
(5) They also had speakers, long before boomboxes and mobile phones pushed sounds out in public.
(6) The elderly groups' variability across the three muscles paralleled that of the 4-yr.-olds, suggesting that speech-motor equivalence returns to an earlier level of operation in aging speakers.
(7) But congressional aides said that House speaker John Boehner has not communicated his intentions for a floor vote to Sensenbrenner.
(8) In the wake of her win, Aung San Suu Kyi has written to Min Aung Hlaing, the president, Thein Sein, and the parliamentary Speaker, Shwe Mann, requesting a meeting to discuss the election and “national reconciliation”, according to the National League for Democracy Facebook page.
(9) And you’re doing it three weeks after the initial revelations, and only when your position is obviously under threat and with a no confidence motion in your position as Speaker looming.
(10) The Republican House speaker John Boehner and the Democratic Senate leader Harry Reid both expressed a desire on Wednesday to work together.
(11) This study explores the power of intonation to convey meaningful information about the communicative intent of the speaker in speech addressed to preverbal infants and in speech addressed to adults.
(12) Some of these grime artists, if they’re telling you to vote, young people are going to listen.” Facebook Twitter Pinterest “Preach!” Speakers on the Grime 4 Corbyn panel debate.
(13) One speaker at an international conference in Bodrum this week asked what would have happened if Turkey had been held closer by the EU?
(14) Other speakers included Shami Chakrabarti , director of the human rights group Liberty, and the Labour MP Jeremy Corbyn who is on the Commons justice select committee.
(15) "I think that Joseph Kabila could go down in history ... if he were to say 'I'm a good sport and I lost,'" said opposition candidate Vital Kamerhe, a former speaker of Parliament.
(16) Ministers can glean vital gossip about cabinet reshuffles if they keep on the right side of their drivers, who form the most high-class grapevine in Britain as they wait in the Speaker's courtyard at Westminster while their charges vote in the Commons.
(17) One of the few Tories who backed him for Speaker says that his increasingly aggressive put-downs of backbenchers have begun to alienate colleagues.
(18) A Benn family spokesperson said: "At the suggestion of the Speaker of the House of Commons and by agreement with the Lords Speaker, Black Rod and the dean of Westminster Abbey, an approach was made by Black Rod to the palace for agreement that Mr Benn's body rest in the chapel of St Mary Undercroft on the night before his funeral.
(19) Regardless of sex, listeners tended to underestimate the age of the speakers.
(20) A number of expert-speakers made recommendations on the basis of currently available information.