(1) We used the polymerase chain reaction (PCR) to amplify the breakpoint area of alpha-thalassemia-1 of Southeast Asia type and several parts of the alpha-globin gene cluster to make a differential diagnosis between alpha-thalassemia-1 and Hb Bart's hydrops fetalis.
(2) We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
(3) We conclude that only one of the alleles was amplified and that either allele could be amplified with respect to both the SphI and PvuII polymorphisms.
(4) RNA preparations were reverse-transcribed, amplified by a polymerase chain reaction (PCR), and separated by electrophoresis.
(5) It was previously demonstrated that multidrug-resistant Chinese hamster cell lines contain an amplified, transcriptionally active DNA sequence designated mdr.
(6) Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) (iii) The insertion plasmid pRSD102(R388::Tn1721) has conserved the capacity of the original plasmid pRSD1 to amplify the 3.55 Mdal-tet region.
(9) To identify the molecular defect in a patient with the intermediate form of the disease, arylsulfatase B mRNA from his fibroblasts was reverse-transcribed, amplified by the polymerase chain reaction, and subcloned.
(10) In addition to the fatigue tester and the pulse duplicator, a signal conditioner, a DC amplifier, an analog-to-digital converter, and a digital microcomputer comprised the essential hardware.
(11) The sensitivity of a PCR system to amplify the long terminal repeat (LTR) sequence of HIV-1 was not affected by the irradiation procedure; however, the ultimate sensitivity of a PCR system for the amplification of an early gene promotor sequence of the CMV genome was reduced 1000-fold.
(12) Thus MTX combined with PALA selected cells with an 'amplificator' phenotype (an increased ability to amplify parts of the genome).
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) Whether we would use that to support and amplify the community ideals already present or go the way of gentrification remained to be seen.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) The action of exogenous etiologic agents is usually mediated in part or amplified by endogenous mediators that very often exert biphasic, i.e., damaging and protective, effects.
(19) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
(20) The PCR amplified a 375-bp DNA fragment which was cloned and sequenced; the deduced amino acid sequence had significant identity with known TS sequences, including strict conservation of all phylogenetically invariant TS amino acid residues.
Tuner
Definition:
(n.) One who tunes; especially, one whose occupation is to tune musical instruments.
Example Sentences:
(1) Kinetic analysis shows that this scheme reduces errors at 'hungry' codons considerably more effectively than J. Ninio's accuracy tuner model; for example, a 10-fold decrease in cognate aminoacyl-tRNA elicits only a 10% increase in the error frequency.
(2) Spearman's Rho correlations indicated that measures taken with the autochromatic tuner significantly correlated with measures taken with the other instruments.
(3) One transmits at 100 MHz and its signal can be received on a standard FM tuner.
(4) Hence, the pineal gland may function as a "fine tuner" of calcium homeostasis.
(5) Each receiver consists of a demodulator, a channel selectable tuner, and a video monitor.
(6) On the basis of the results, a role is attributed to the decreased growth hormone reserve in the low growth of Tuner's syndrome patients.
(7) Thus the modulator may function as a biologic "fine tuner" providing on additional mechanism by which the signals imparted to cells by physiologic stimuli can be correctly expressed.
(8) It is no longer enough simply to play music (or, as non-iPod devices often can, have an FM tuner and voice recorder).
(9) The author coined the term 'tuning' for the reflex hypertonia of tensor palati which is directly proportional to the degree of the slackness of its 'tuner', the muscle-tensor tympani.
(10) "First, the government must ensure that advice goes to retailers and the public that when purchasing radios, consumers should purchase sets that include a digital tuner.
(11) Hence, adaptive controllers in the form of linear and nonlinear generalised minimum variance self-tuners, generalised predictive control and nonlinear k-step ahead predictive controllers are also considered.
(12) No one from SST's glory days seems to have a good word to say about founder Greg Ginn , who expanded his radio parts operation Solid State Tuners in 1978 so he could put out a record, Nervous Breakdown, by his band Black Flag.
(13) The purpose of these measures was to determine whether an autochromatic tuner, a relatively inexpensive device designed to assist musicians in fast-tuning their instruments, would provide a valid and reliable measure of vocal fundamental frequency.
(14) Results indicate that the use of an autochromatic tuner to measure vocal fundamental frequency is an effective and inexpensive alternative to other methods for clinical purposes.
(15) The system used a Konigsberg P7 transducer, a temperature-compensated voltage-controlled oscillator, an FM transmitter, and an FM tuner to convert the signal received into a voltage proportional to aortic blood pressure.