(1) Immunoreactivity of antisera directed against human neuropeptide Y (NPY), anglerfish polypeptide YG (aPY), bovine pancreatic polypeptide (bPP), salmon pancreatic polypeptide (sPP), porcine peptide tyrosine tyrosine (PYY), and salmon glucagon-like peptide (GLP) was investigated in the endocrine pancreas and anterior intestine of adult lampreys, Petromyzon marinus, by immunohistochemical analysis.
(2) Anglerfish peptide YG (aPY) was isolated from pancreatic islets of the anglerfish.
(3) The results demonstrate that a PAM is present in secretory granules of anglerfish islet tissue.
(4) Two distinct somatostatin precursors are synthesized in anglerfish (AF) islets.
(5) The results suggest that the two somatostatin genes present in both anglerfish and catfish were the result of a gene duplication event in a common ancestor of anglerfish and catfish.
(6) Although the functional significance of this observation remains to be determined, the results imply that oxytocin, or an oxytocin-like peptide, may affect the synthesis or release of insulin from anglerfish islets.
(7) We have used a recombinant retroviral expression vector encoding anglerfish prepor-SRIF-I to infect rat pituitary GH3 cells.
(8) Amino acid incorporation into SLI has been studied in a variety of systems, including anglerfish and rat pancreas, frog retina, rat dorsal root ganglia, cerebral cortex and hypothalamus.
(9) A propeptide converting enzyme capable of producing somatostatin-14 has been identified and partially characterized from anglerfish pancreatic islets.
(10) The effects of glucose and arginine on islet hormone biosynthesis were investigated using primary cell cultures prepared from islets of the anglerfish (Lophius americanus).
(11) Anglerfish islet somatostatin was found to possess an amino acid composition and immunological and biological activities equivalent to synthetic somatostatin.
(12) As part of an effort to characterize prohormone processing enzymes in the anglerfish, Lophius americanus, we have cloned and sequenced a cDNA for the fish prohormone processing carboxypeptidase H (CPH).
(13) Subsequent immunohistochemical and biochemical analyses demonstrated that anglerfish islet cells synthesize aPY.
(14) An enzyme that performs the conversion of anglerfish prosomatostatin-II (pro-SS-II) to anglerfish SS-28 has been identified using an improved two-dimensional electrophoresis procedure.
(15) In the flounder the same dibasic residue processing site is utilised but cleavage at different monobasic sites takes place (corresponding to Arg50 and Arg97 in anglerfish preprosomatostatin II).
(16) Somatostatin-28 was the major form of somatostatin detected and is similar to the gene II product from anglerfish.
(17) They suggest that this octacosapeptide, which is potent on the inhibition of growth hormone release by anterior pituitary cells, may play such a role in the gastrointestinal tract of the anglerfish.
(18) Results from a previous report demonstrate that more than one molecular form of neuropeptide Y-like peptide may be present in the islet organ of the anglerfish (Lophius americanus).
(19) In pancreatic islets of the anglerfish (Lophius americanus), there are two separate precursors, preproSRIF I and preproSRIF II, which give rise to SRIF-14 or an N-terminally extended form SRIF-28, respectively.
(20) The results demonstrate the usefulness of the culture system for studying the modulation of hormone biosynthesis in anglerfish islet cells.