(v. t.) To subject to great heat, and then cool slowly, as glass, cast iron, steel, or other metal, for the purpose of rendering it less brittle; to temper; to toughen.
(v. t.) To heat, as glass, tiles, or earthenware, in order to fix the colors laid on them.
Example Sentences:
(1) Single-stranded circles did not form if a limited number of nucleotides were removed from the 3' ends of native molecules by Escherichia coli exonuclease III digestion prior to denaturation and annealing.
(2) Single-stranded linear DNAs were prepared by separating strands of duplex molecules or by cleaving single-stranded circles at a unique restriction site created by annealing a short defined oligonucleotide to the circle.
(3) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(4) The solution conformation of an antibacterial protein sapecin has been determined by 1H nuclear magnetic resonance (NMR) and dynamical simulated annealing calculations.
(5) The UV sensitivity was found to increase with increasing preliminary gamma exposure above 10(-2)Ckg-1 (after a post-irradiation anneal at 300 degrees C for 1 h).
(6) The addition of Mg2+ to the dialyzing solution resulted in the formation of short intermediate-sized filaments even at 4 degrees C. Further dialysis of the short intermediate-sized filaments against reassembly solution containing both NaCl and MgCl2 at 37 degrees C failed to elongate them into longer filaments, suggesting that annealing does not contribute to the elongation of neurofilaments.
(7) The crystal structure of the complex between neuraminidase from influenza virus (subtype N9 and isolated from an avian source) and the antigen-binding fragment (Fab) of monoclonal antibody NC41 has been refined by both least-squares and simulated annealing methods to an R-factor of 0.191 using 31,846 diffraction data in the resolution range 8.0 to 2.5 A.
(8) The transcript, analyzed by DNA-RNA hybridization, shows two major kinetic components: 40% of the transcript is copied by repetitive sequences present 100 times per haploid genome; another 25% anneals to DNA with a rate constant Kh 10-4 M - S-1, typical of single-copy sequences.
(9) Furthermore, globin mRNA has a 3'-terminal sequence of adenylic acid residues that make it particularly suitable as a template, since oligo(dT) can be annealed to a specific site on the mRNA.
(10) The thermal cycle sequencing procedures are advantageous because they allow fast and simple semiautomation of the sequencing reaction; make possible the direct DNA sequencing of PCR products, bacterial colonies and phage plaques; require only femtomoles of template DNA; eliminate the requirement of an independent primer annealing step; remove the requirement of denatured plasmids for sequencing double-stranded templates; and use a highly thermostable DNA polymerase for sequencing through potential recalcitrant secondary structure domains and large linear double-stranded DNA templates such as lambda derivatives.
(11) When 18 to 19S and 12 to 15S viral RNAs purified by sucrose gradient centrifugation were similarly analyzed, late RNA inhibited hybridization of 18 to 19S RNA 50%, and the annealing of 12 to 15S RNA was inhibited 70%.
(12) Partial degradation of these DNA molecules by exonuclease III predisposes some of them to form circles upon annealing, but indicating they are terminally redundant.
(13) The procedures are only intermediate phases and are without any significant role, since the end-result is, after annealing and polishing, as expected.
(14) The results indicate that the relaxed circles consist of a population containing one interruption in either of the two strands of the duplex jointly representing the two "nicks" contained in Hershey circles (in which the cohesive ends are annealed).
(15) The method is based on the priming of polymerase chain reaction (PCR) by an oligonucleotide complementary to the DNA sequence containing the mutation of interest, which anneals only to the perfectly matched sequence under high stringency conditions.
(16) Molecular probes from the gp150 locus annealed with a 4.0-kb polyadenylated RNA transcript derived from human myeloid cell lines and from tertiary mouse cell transformants.
(17) spectra at various annealing stages from alpha- and beta-D-glucose together with 6,6-d2-D-glucose, 6-deoxy-D-glucose, 2-deoxy-D-glucose, glucose-1-phosphate, D-xylose, D-allose and D-mannose indicates radical formation at all possible carbon sites with a strong preference for C1 and a somewhat enhanced contribution of C4 over the statistical expectation.
(18) After gel electrophoresis and blotting the amplification product is identified by hybridization with a third oligonucleotide recognizing the region between the two primer annealing sites, and by restriction mapping.
(19) In this article we experimentally determine the optimal annealing temperature (TaOPT) values for several primer-template pairs and develop a method for its calculation.
(20) Use of the sequence annealing in the calorimetric measurement allows to decompose the total thermogram of rod into four elementary bands with maxima at 42, 46.5, 50 and 57 degrees C. Fluorescence changes occur at temperatures which coincide with the first, second and fourth calorimetric peaks.
Heat
Definition:
(n.) A force in nature which is recognized in various effects, but especially in the phenomena of fusion and evaporation, and which, as manifested in fire, the sun's rays, mechanical action, chemical combination, etc., becomes directly known to us through the sense of feeling. In its nature heat is a mode if motion, being in general a form of molecular disturbance or vibration. It was formerly supposed to be a subtile, imponderable fluid, to which was given the name caloric.
(n.) The sensation caused by the force or influence of heat when excessive, or above that which is normal to the human body; the bodily feeling experienced on exposure to fire, the sun's rays, etc.; the reverse of cold.
(n.) High temperature, as distinguished from low temperature, or cold; as, the heat of summer and the cold of winter; heat of the skin or body in fever, etc.
(n.) Indication of high temperature; appearance, condition, or color of a body, as indicating its temperature; redness; high color; flush; degree of temperature to which something is heated, as indicated by appearance, condition, or otherwise.
(n.) A single complete operation of heating, as at a forge or in a furnace; as, to make a horseshoe in a certain number of heats.
(n.) A violent action unintermitted; a single effort; a single course in a race that consists of two or more courses; as, he won two heats out of three.
(n.) Utmost violence; rage; vehemence; as, the heat of battle or party.
(n.) Agitation of mind; inflammation or excitement; exasperation.
(n.) Animation, as in discourse; ardor; fervency.
(n.) Sexual excitement in animals.
(n.) Fermentation.
(v. t.) To make hot; to communicate heat to, or cause to grow warm; as, to heat an oven or furnace, an iron, or the like.
(v. t.) To excite or make hot by action or emotion; to make feverish.
(v. t.) To excite ardor in; to rouse to action; to excite to excess; to inflame, as the passions.
(v. i.) To grow warm or hot by the action of fire or friction, etc., or the communication of heat; as, the iron or the water heats slowly.
(v. i.) To grow warm or hot by fermentation, or the development of heat by chemical action; as, green hay heats in a mow, and manure in the dunghill.
(imp. & p. p.) Heated; as, the iron though heat red-hot.
Example Sentences:
(1) Tryptic digestion of the membranes caused complete disappearance of the binding activity, but heat-treatment for 5 min at 70 degrees C caused only 40% loss of activity.
(2) A new and simple method of serotyping campylobacters has been developed which utilises co-agglutination to detect the presence of heat-stable antigens.
(3) The 40 degrees C heating induced an increase in systolic, diastolic, average and pulse pressure at rectal temperature raised to 40 degrees C. Further growth of the body temperature was accompanied by a decrease in the above parameters.
(4) The effect of heat on glucocorticoids of plasma was not significant.
(5) This Mr 20,000 inhibitory activity was acid and heat stable and sensitive to dithiothreitol and trypsin.
(6) There is a relationship between the duration of stimulation (t) and the total heat production (H) of the type H = A plus bt, where A and b are constants.
(7) This suggests that there was a deterioration of the vasoconstrictor response and indicated a possible effect of heat at the receptor or effector level.
(8) While both inhibitors caused thermosensitization, they did not affect the time scale for the development of thermotolerance at 42 degrees C or after acute heating at 45 degrees C. The inhibitors of poly(ADP-ribosylation) radiosensitizers and thermosensitizers may be of use in the treatment of cancer using a combined modality of radiation and hyperthermia.
(9) The binding to DNA-cellulose of heat-activated [3H]RU486-receptor complexes was slightly decreased (37%) when compared with that of the agonist [3H]R5020-receptor complexes (47%).
(10) By means of rapid planar Hill type antimony-bismuth thermophiles the initial heat liberated by papillary muscles was measured synchronously with developed tension for control (C), pressure-overload (GOP), and hypothyrotic (PTU) rat myocardium (chronic experiments) and after application of 10(-6) M isoproterenol or 200 10(-6) M UDCG-115.
(11) The return of NE to normal levels after one month is consistent with the observation that LH-lesioned rats are by one month postlesion no longer hypermetabolic, but display levels of heat production appropriate to the reduced body weight they then maintain.
(12) It is the action of this protease that releases the enzyme from the membrane, as shown by the observations that protease inhibitors decreased the amount of solubilization of the enzyme, and the enzyme remaining in the membrane after heating showed much less proteolytic cleavage than that which was released.
(13) The apparent sensitivity of Escherichia coli K12 to mild heat was increased by recA (def), recB and polA, but not by uvrA, uvrB or recF mutations.
(14) Michele Hanson 'The heat finally broke – I realised something had to change …' Stuart Heritage (right) with his brother in 2003.
(15) The data suggest that inhibition of gain in weight with the addition of pyruvate and dihydroxyacetone to the diet is the result of an increased loss of calories as heat at the expense of storage as lipid.
(16) Induction of both potential transcripts follows heat shock in vivo.
(17) Lebedev punched Polonsky during a heated early recording of NTVshniki.
(18) At the site of injury heat itself causes microvascular damage.
(19) Acid-fast bacilli were isolated from 3 out of 41 mice inoculoted with heat killed bacilli.
(20) Mean run time and total ST time were faster with CE (by 1.4 and 1.2 min) although not significantly different (P less than 0.06 and P less than 0.10) from P. Subjects reported no significant difference in nausea, fullness, or stomach upset with CE compared to P. General physiological responses were similar for each drink during 2 h of multi-modal exercise in the heat; however, blood glucose, carbohydrate utilization, and exercise intensity at the end of a ST may be increased with CE fluid replacement.