What's the difference between annealing and glass?

Annealing


Definition:

  • (p. pr. & vb. n.) of Anneal
  • (n.) The process used to render glass, iron, etc., less brittle, performed by allowing them to cool very gradually from a high heat.
  • (n.) The burning of metallic colors into glass, earthenware, etc.

Example Sentences:

  • (1) Single-stranded circles did not form if a limited number of nucleotides were removed from the 3' ends of native molecules by Escherichia coli exonuclease III digestion prior to denaturation and annealing.
  • (2) Single-stranded linear DNAs were prepared by separating strands of duplex molecules or by cleaving single-stranded circles at a unique restriction site created by annealing a short defined oligonucleotide to the circle.
  • (3) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
  • (4) The solution conformation of an antibacterial protein sapecin has been determined by 1H nuclear magnetic resonance (NMR) and dynamical simulated annealing calculations.
  • (5) The UV sensitivity was found to increase with increasing preliminary gamma exposure above 10(-2)Ckg-1 (after a post-irradiation anneal at 300 degrees C for 1 h).
  • (6) The addition of Mg2+ to the dialyzing solution resulted in the formation of short intermediate-sized filaments even at 4 degrees C. Further dialysis of the short intermediate-sized filaments against reassembly solution containing both NaCl and MgCl2 at 37 degrees C failed to elongate them into longer filaments, suggesting that annealing does not contribute to the elongation of neurofilaments.
  • (7) The crystal structure of the complex between neuraminidase from influenza virus (subtype N9 and isolated from an avian source) and the antigen-binding fragment (Fab) of monoclonal antibody NC41 has been refined by both least-squares and simulated annealing methods to an R-factor of 0.191 using 31,846 diffraction data in the resolution range 8.0 to 2.5 A.
  • (8) The transcript, analyzed by DNA-RNA hybridization, shows two major kinetic components: 40% of the transcript is copied by repetitive sequences present 100 times per haploid genome; another 25% anneals to DNA with a rate constant Kh 10-4 M - S-1, typical of single-copy sequences.
  • (9) Furthermore, globin mRNA has a 3'-terminal sequence of adenylic acid residues that make it particularly suitable as a template, since oligo(dT) can be annealed to a specific site on the mRNA.
  • (10) The thermal cycle sequencing procedures are advantageous because they allow fast and simple semiautomation of the sequencing reaction; make possible the direct DNA sequencing of PCR products, bacterial colonies and phage plaques; require only femtomoles of template DNA; eliminate the requirement of an independent primer annealing step; remove the requirement of denatured plasmids for sequencing double-stranded templates; and use a highly thermostable DNA polymerase for sequencing through potential recalcitrant secondary structure domains and large linear double-stranded DNA templates such as lambda derivatives.
  • (11) When 18 to 19S and 12 to 15S viral RNAs purified by sucrose gradient centrifugation were similarly analyzed, late RNA inhibited hybridization of 18 to 19S RNA 50%, and the annealing of 12 to 15S RNA was inhibited 70%.
  • (12) Partial degradation of these DNA molecules by exonuclease III predisposes some of them to form circles upon annealing, but indicating they are terminally redundant.
  • (13) The procedures are only intermediate phases and are without any significant role, since the end-result is, after annealing and polishing, as expected.
  • (14) The results indicate that the relaxed circles consist of a population containing one interruption in either of the two strands of the duplex jointly representing the two "nicks" contained in Hershey circles (in which the cohesive ends are annealed).
  • (15) The method is based on the priming of polymerase chain reaction (PCR) by an oligonucleotide complementary to the DNA sequence containing the mutation of interest, which anneals only to the perfectly matched sequence under high stringency conditions.
  • (16) Molecular probes from the gp150 locus annealed with a 4.0-kb polyadenylated RNA transcript derived from human myeloid cell lines and from tertiary mouse cell transformants.
  • (17) spectra at various annealing stages from alpha- and beta-D-glucose together with 6,6-d2-D-glucose, 6-deoxy-D-glucose, 2-deoxy-D-glucose, glucose-1-phosphate, D-xylose, D-allose and D-mannose indicates radical formation at all possible carbon sites with a strong preference for C1 and a somewhat enhanced contribution of C4 over the statistical expectation.
  • (18) After gel electrophoresis and blotting the amplification product is identified by hybridization with a third oligonucleotide recognizing the region between the two primer annealing sites, and by restriction mapping.
  • (19) In this article we experimentally determine the optimal annealing temperature (TaOPT) values for several primer-template pairs and develop a method for its calculation.
  • (20) Use of the sequence annealing in the calorimetric measurement allows to decompose the total thermogram of rod into four elementary bands with maxima at 42, 46.5, 50 and 57 degrees C. Fluorescence changes occur at temperatures which coincide with the first, second and fourth calorimetric peaks.

Glass


Definition:

  • (v. t.) A hard, brittle, translucent, and commonly transparent substance, white or colored, having a conchoidal fracture, and made by fusing together sand or silica with lime, potash, soda, or lead oxide. It is used for window panes and mirrors, for articles of table and culinary use, for lenses, and various articles of ornament.
  • (v. t.) Any substance having a peculiar glassy appearance, and a conchoidal fracture, and usually produced by fusion.
  • (v. t.) Anything made of glass.
  • (v. t.) A looking-glass; a mirror.
  • (v. t.) A vessel filled with running sand for measuring time; an hourglass; and hence, the time in which such a vessel is exhausted of its sand.
  • (v. t.) A drinking vessel; a tumbler; a goblet; hence, the contents of such a vessel; especially; spirituous liquors; as, he took a glass at dinner.
  • (v. t.) An optical glass; a lens; a spyglass; -- in the plural, spectacles; as, a pair of glasses; he wears glasses.
  • (v. t.) A weatherglass; a barometer.
  • (v. t.) To reflect, as in a mirror; to mirror; -- used reflexively.
  • (v. t.) To case in glass.
  • (v. t.) To cover or furnish with glass; to glaze.
  • (v. t.) To smooth or polish anything, as leater, by rubbing it with a glass burnisher.

Example Sentences:

  • (1) The previous year, he claimed £1,415 for two new sofas, made two separate claims of £230 and £108 for new bed linen, charged £86 for a new kettle and kitchen utensils and made two separate claims, of £65 and £186, for replacement glasses and crockery.
  • (2) Human gingival fibroblasts were allowed to attach and spread on bio-glasses for 1-72 h. Unreactive silica glass and cell culture polystyrene served as controls.
  • (3) Retention of platelets from whole blood on glass beads was performed by the method of Bowie.
  • (4) Populations of lymphocytes were separated using glass and nylon wool.
  • (5) Analysis of bond values of glass ionomer added to glass ionomer indicate bond variability and low cohesive bond strength of the material.
  • (6) It was like watching somebody pouring a blue liquid into a glass, it just began filling up.
  • (7) A reference glass, five ceramic materials, and one resin-based composite were tested.
  • (8) The average repetitive yields and initial coupling of proteins spotted or blotted into PVDF membranes ranged between 84-98% and 30-108% respectively, and were comparable with the yields measured for proteins spotted onto Polybrene-coated glass fiber discs.
  • (9) Samples of rockwool and glass fibre were compared with chrysotile fibres for their capacity to hydroxylate 2-deoxyguanosine to 8-hydroxydeoxyguanosine, a reaction that is mediated by formation of hydroxyl radicals.
  • (10) Perfused or immersion-fixed epithalamic tissues, sectioned, and mounted on glass slides were processed through the avidin-biotin immunofluorescence method.
  • (11) Nango's dwellings are built on skis so can be pulled around the beach, and have a glass roof to view the northern lights.
  • (12) His office - with a floor-to-ceiling glass wall offering views over a Bradford suburb and distant moors - is devoid of knick-knacks or memorabilia.
  • (13) Three brands of glass ionomer were applied to prepared dentin surfaces of extracted human molars, after one of four treatments with polyacrylic acid.
  • (14) At the bottom is a tiny harbour where cafe Itxas Etxea – bare brick walls and wraparound glass windows – is serving txakoli, the local white wine.
  • (15) When Vladimir Putin kicks back on New Year's Eve with a glass of Russian-made champagne, and reflects on the year behind him, he is likely to feel rather pleased with himself at the way his foreign policy initiatives have gone in 2013.
  • (16) When used in snail neurones such electrodes gave very similar pHi values to those recorded simultaneously by recessed-tip glass micro-electrodes.
  • (17) Cells dissociated from 6-day rat cerebellum were seeded on glass coverslips coated with polylysine on one half and hyaluronectin on the other.
  • (18) These results confirmed that 'punctuated' labeling was not an artefact due to a distortion of the cell's shape by having been dried on glass slides.
  • (19) At one, in the Gun and Dog pub in Leeds on Tuesday, a witness described how the meeting descended into chaos when one of the rebels smashed a glass and threatened to attack Griffin supporter Mark Collett.
  • (20) Dissociated culture of adult mouse dorsal root ganglion cells on glass plates, on which grating-associated microstructures (a repetition of microgrooves [mGRV] and microsteps [mSTP] of 0.1-10 micron) are fabricated by the conventional lithographic techniques, represents a remarkable bi-directional growth of their nerve fibers in the axial direction of the grating.

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