(n.) The process used to render glass, iron, etc., less brittle, performed by allowing them to cool very gradually from a high heat.
(n.) The burning of metallic colors into glass, earthenware, etc.
Example Sentences:
(1) Single-stranded circles did not form if a limited number of nucleotides were removed from the 3' ends of native molecules by Escherichia coli exonuclease III digestion prior to denaturation and annealing.
(2) Single-stranded linear DNAs were prepared by separating strands of duplex molecules or by cleaving single-stranded circles at a unique restriction site created by annealing a short defined oligonucleotide to the circle.
(3) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(4) The solution conformation of an antibacterial protein sapecin has been determined by 1H nuclear magnetic resonance (NMR) and dynamical simulated annealing calculations.
(5) The UV sensitivity was found to increase with increasing preliminary gamma exposure above 10(-2)Ckg-1 (after a post-irradiation anneal at 300 degrees C for 1 h).
(6) The addition of Mg2+ to the dialyzing solution resulted in the formation of short intermediate-sized filaments even at 4 degrees C. Further dialysis of the short intermediate-sized filaments against reassembly solution containing both NaCl and MgCl2 at 37 degrees C failed to elongate them into longer filaments, suggesting that annealing does not contribute to the elongation of neurofilaments.
(7) The crystal structure of the complex between neuraminidase from influenza virus (subtype N9 and isolated from an avian source) and the antigen-binding fragment (Fab) of monoclonal antibody NC41 has been refined by both least-squares and simulated annealing methods to an R-factor of 0.191 using 31,846 diffraction data in the resolution range 8.0 to 2.5 A.
(8) The transcript, analyzed by DNA-RNA hybridization, shows two major kinetic components: 40% of the transcript is copied by repetitive sequences present 100 times per haploid genome; another 25% anneals to DNA with a rate constant Kh 10-4 M - S-1, typical of single-copy sequences.
(9) Furthermore, globin mRNA has a 3'-terminal sequence of adenylic acid residues that make it particularly suitable as a template, since oligo(dT) can be annealed to a specific site on the mRNA.
(10) The thermal cycle sequencing procedures are advantageous because they allow fast and simple semiautomation of the sequencing reaction; make possible the direct DNA sequencing of PCR products, bacterial colonies and phage plaques; require only femtomoles of template DNA; eliminate the requirement of an independent primer annealing step; remove the requirement of denatured plasmids for sequencing double-stranded templates; and use a highly thermostable DNA polymerase for sequencing through potential recalcitrant secondary structure domains and large linear double-stranded DNA templates such as lambda derivatives.
(11) When 18 to 19S and 12 to 15S viral RNAs purified by sucrose gradient centrifugation were similarly analyzed, late RNA inhibited hybridization of 18 to 19S RNA 50%, and the annealing of 12 to 15S RNA was inhibited 70%.
(12) Partial degradation of these DNA molecules by exonuclease III predisposes some of them to form circles upon annealing, but indicating they are terminally redundant.
(13) The procedures are only intermediate phases and are without any significant role, since the end-result is, after annealing and polishing, as expected.
(14) The results indicate that the relaxed circles consist of a population containing one interruption in either of the two strands of the duplex jointly representing the two "nicks" contained in Hershey circles (in which the cohesive ends are annealed).
(15) The method is based on the priming of polymerase chain reaction (PCR) by an oligonucleotide complementary to the DNA sequence containing the mutation of interest, which anneals only to the perfectly matched sequence under high stringency conditions.
(16) Molecular probes from the gp150 locus annealed with a 4.0-kb polyadenylated RNA transcript derived from human myeloid cell lines and from tertiary mouse cell transformants.
(17) spectra at various annealing stages from alpha- and beta-D-glucose together with 6,6-d2-D-glucose, 6-deoxy-D-glucose, 2-deoxy-D-glucose, glucose-1-phosphate, D-xylose, D-allose and D-mannose indicates radical formation at all possible carbon sites with a strong preference for C1 and a somewhat enhanced contribution of C4 over the statistical expectation.
(18) After gel electrophoresis and blotting the amplification product is identified by hybridization with a third oligonucleotide recognizing the region between the two primer annealing sites, and by restriction mapping.
(19) In this article we experimentally determine the optimal annealing temperature (TaOPT) values for several primer-template pairs and develop a method for its calculation.
(20) Use of the sequence annealing in the calorimetric measurement allows to decompose the total thermogram of rod into four elementary bands with maxima at 42, 46.5, 50 and 57 degrees C. Fluorescence changes occur at temperatures which coincide with the first, second and fourth calorimetric peaks.
Metal
Definition:
(n.) An elementary substance, as sodium, calcium, or copper, whose oxide or hydroxide has basic rather than acid properties, as contrasted with the nonmetals, or metalloids. No sharp line can be drawn between the metals and nonmetals, and certain elements partake of both acid and basic qualities, as chromium, manganese, bismuth, etc.
(n.) Ore from which a metal is derived; -- so called by miners.
(n.) A mine from which ores are taken.
(n.) The substance of which anything is made; material; hence, constitutional disposition; character; temper.
(n.) Courage; spirit; mettle. See Mettle.
(n.) The broken stone used in macadamizing roads and ballasting railroads.
(n.) The effective power or caliber of guns carried by a vessel of war.
(n.) Glass in a state of fusion.
(n.) The rails of a railroad.
(v. t.) To cover with metal; as, to metal a ship's bottom; to metal a road.
Example Sentences:
(1) In this study of ten consecutive patients sustaining molten metal injuries to the lower extremity who were treated with excision and grafting, treatment with compression Unna paste boot was compared with that with conventional dressing.
(2) The LD50 of the following metal-binding chelating drugs, EDTA, diethylenetriaminepentaacetic acid (DTPA), hydroxyethylenediaminetriacetic acid (HEDTA), cyclohexanediaminotetraacetic acid (CDTA) and triethylenetetraminehexaacetic acid (TTHA) was evaluated in terms of mortality in rats after intraperitoneal administration and was found to be in the order: CDTA greater than EDTA greater than DTPA greater than TTHA greater than HEDTA.
(3) Our recurrences are due to local infections, removing the metal strut too early, i.e.
(4) "Acoustic" craters were produced by two laser pulses delivered into a saline-filled metal fiber cap, which was placed in a mechanically drilled crater.
(5) These results indicate that the inhibition of CarAc by heavy metals occurs by binding of the sulfhydryl on the enzyme by the metals.
(6) The consequences of proved hypersensitivity in patients with metal-to-plastic prostheses, either present prior to insertion of the prosthesis or evoked by the implant material, are not known.
(7) In general, enzyme activity was strongly reduced by heavy metal inorganic cations; less strongly by organometallic cations, some anions, and certain pesticides; and weakly inhibited by light metal cations and organometallic and organic compounds.
(8) EGTA was ineffective in removing calmodulin from particulate preparations, but treatment with the tervalent metal ion La3+ resulted in a loss of up to 98% of calmodulin activity from these preparations.
(9) Sift the cocoa powder over the top and lightly but thoroughly fold it in with the metal spoon.
(10) The results also suggest that both alkali metals most probably have been delivered to the suckling pups and some of their toxic effect was retarded.
(11) The radioprotective action in E. coli ATCC 9637 of ascorbate added to media containing the weak sensitizer, tetracycline (effect described by Pittillo and Lucas (1967)), was found to be dependent on the presence of metal catalysts of the autoxidation of ascorbate.
(12) This study introduces a simple in vitro arrangement to measure current densities of implant metals.
(13) A rubber cuff was fixed on the metal cylinder and let an opening of 8 cm, simulating the cervix uteri.
(14) Since the enzyme requires a metal ion (Co2+) we suggest that the RNA and heparin are inhibitory by virtue of their capacity to chelate the Co2+.
(15) Lesions of allergic contact type could not be induced in the oral mucosa to any of the metal salt preparations.
(16) As yet the observations demonstrate that workers exposed in their occupation to heavy metals (cadmium, lead, metalic mercury) and organic solvents should be subjected to special control for detection of renal changes.
(17) This study investigates the photoneutron field found in medical accelerator rooms with primary barriers constructed of metal slabs plus concrete.
(18) While a clearcut relationship cannot be established between heavy metal music and destructive behavior, evidence shows that such music promotes and supports patterns of drug abuse, promiscuous sexual activity, and violence.
(19) The physiologically important metal ion for catalysis is Mg2+; however, Mn2+ supports in vitro activity, though at a reduced level.
(20) We support the view that catalysis by metalloenzymes may be a reflection of the chemistry of the metal ion itself as a Lewis acid, and that perhaps too much emphasis has been placed on supposed special characteristics (such as strains, "entasis") of the enzyme-metal ion association.