(n.) Rubbish or refuse consisting of broken rock containing little or no ore.
Example Sentences:
(1) Similar analyses demonstrate that efficient excision can occur with two other different sets of wild-type arm-type sites in attL and attR.
(2) The results indicate that pSG1int is flanked at attL by a functional tRNA(ser) gene and at attR by a 60-nt sequence of the 3' end of the same tRNA(ser) gene.
(3) A 1200-bp region of P2 DNA containing the int gene and attP, the prophage hybrid ends attL and attR, and one bacterial attachment site, the preferred site locI from E. coli strain C, have all been sequenced.
(4) Moreover, lambda DNA containing a pBR322 derivative flanked by the lambda attL and attR sites could be specifically recircularized by excisive lambda recombination to yield the pBR322 derivative.
(5) Excision (intraplasmid att site recombination) was examined by constructing plasmids carrying attL and attR or two attP sites separating CmR from KmR and by following segregation of the markers in various hosts.
(6) To map the protein-protein and protein-DNA interactions involved in lambda site-specific recombination, Int cleavage assays with suicide substrates, nuclease protection patterns, gel retardation experiments, and quantitative Western blotting were applied to wild-type attL and attL mutants.
(7) The pSG1 plasmid integration site (attP) and the pSG1int-chromosome boundaries (attL and attR) were cloned and sequenced.
(8) Similar plasmids containing the two junction segments (attL and attR regions) between the phage genome and the lysogenic host chromosome were also prepared.
(9) The results lead to a model in which one IHF molecule bends the attL DNA and forms a higher order complex with the three bivalent Int molecules required for excisive recombination.
(10) The attL of the second bacterial attachment site present in the host SmaI fragment 7 and the leftmost part of phage S2 type B DNA of its genome organization (Piekarowicz et.
(11) Nucleotide sequencing of attP, attB, attL, and attR revealed a 57-base-pair sequence common to all sites with no duplications of adjacent plasmid or chromosomal sequences in the integrated state, indicating that integration takes place through conservative, reciprocal strand exchange.
(12) Using site-directed mutagenesis, we have constructed a related set of point mutations within each of the five Int "arm-type" binding sites located within attP, attL and attR.
(13) The attachment site of pIJ408 (attP) and the junctions of its integrated form with the chromosomal DNA in S. glaucescens (attL and attR) contain an identical 43 bp sequence.
(14) This recombination was directional, since no reaction was observed between plasmids containing attR and attL sites.
(15) Oligonucleotides derived from the sequence of the attP-containing fragment enabled us to amplify predicted junction fragment sequences and thus to identify attL, attR, and attB.
(16) With these novel substrates we show that Xis specifically promotes the first strand exchange and that attL enhances Int cleavage at the top-strand site of attR.
(17) A 58-bp sequence (att) present in both pSAM2 (attP) and S. ambofaciens strain DSM40697 (attB) attachment regions is found at the boundaries (attL and attR) of integrated pSAM2 in S. ambofaciens strain ATCC23877.
(18) The endpoints of the bacterial DNA segments in lambdagal3 and lambdagal8 are physically mapped in relation to attL.
(19) By combining the attL and attR sequences, the attB sequences of locations II, III, and H have been deduced.
(20) The DNA nucleotide sequences of attP, attB attR and attL were determined.
Refuse
Definition:
(v. t.) To deny, as a request, demand, invitation, or command; to decline to do or grant.
(v. t.) To throw back, or cause to keep back (as the center, a wing, or a flank), out of the regular aligment when troops ar/ about to engage the enemy; as, to refuse the right wing while the left wing attacks.
(v. t.) To decline to accept; to reject; to deny the request or petition of; as, to refuse a suitor.
(v. t.) To disown.
(v. i.) To deny compliance; not to comply.
(n.) Refusal.
(n.) That which is refused or rejected as useless; waste or worthless matter.
(a.) Refused; rejected; hence; left as unworthy of acceptance; of no value; worthless.
Example Sentences:
(1) We were instantly refused entrance by the heavies at the door.
(2) There are widespread examples across the US of the police routinely neglecting crimes of sexual violence and refusing to believe victims.
(3) Such a science puts men in a couple of scientific laws and suppresses the moment of active doing (accepting or refusing) as a sufficient preassumption of reality.
(4) There were no deaths but one refused to have ketamine again.
(5) That’s a criticism echoed by Democrats in the Senate, who issued a report earlier this month criticising Republicans for passing sweeping legislation in July to combat addiction , the Comprehensive Addiction and Recovery Act (Cara), but refusing to fund it.
(6) She successfully appealed against the council’s decision to refuse planning permission, but neighbours have launched a legal challenge to be heard at the high court in June.
(7) Tony Abbott has refused to concede that saying Aboriginal people who live in remote communities have made a “lifestyle choice” was a poor choice of words as the father of reconciliation issued a public plea to rebuild relations with Indigenous people.
(8) The military is not being honest about the number of men on strike: most of us are refusing to eat.
(9) But employers who have followed a fair procedure may have the right to discipline or finally dismiss any smoker who refuses to accept the new rules.
(10) Republican presidential hopeful Scott Walker has refused to say whether he believes in the theory of evolution, arguing that it is “a question a politician shouldn’t be involved in one way or the other”.
(11) But in a setback to the UK, Somaliland, which broke away from Somalia in 1991, refused British entreaties to attend on the grounds that it would not have been treated as equal to the Somali government.
(12) Ten patients had been treated by adrenalectomy, one patient by radiotherapy of the hypophysis, and one patient had refused any treatment.
(13) What if the court of justice refuses to answer the question?
(14) The only thing the media will talk about in the hours and days after the debate will be Trump’s refusal to say he will accept the results of the election, making him appear small, petty and conspiratorial.
(15) A small band of shadow cabinet members have lined up to refuse to serve in posts they haven’t even been offered, on the basis of objection to economic policies they clearly haven’t read.
(16) The prerequisite for all champions is the refusal to cave in, so City's equaliser with only three minutes remaining was pleasing.
(17) Black males with low intentions to use condoms reported significantly more negative attitudes about the use of condoms (eg, using condoms is disgusting) and reacted with more intense anger when their partners asked about previous sexual contacts, when a partner refused sex without a condom, or when they perceived condoms as interfering with foreplay and sexual pleasure.
(18) As long as Israel refuses to cease settlement activities and to the release of the fourth group of Palestinian prisoners in accordance with our agreements, they leave us no choice but to insist that we will not remain the only ones committed to the implementation of these agreements, while Israel continuously violates them,” Abbas said.
(19) The people who will lose are not the commercial interests, and people with particular vested interests, it’s the people who pay for us, people who love us, the 97% of people who use us each week, there are 46 million people who use us every day.” Hall refused to be drawn on what BBC services would be cut as a result of the funding deal which will result in at least a 10% real terms cut in the BBC’s funding.
(20) These letters are also written during a period when Joyce was still smarting from the publishing difficulties of his earlier works Dubliners and A Portrait of the Artist as a Young Man.” Gordon Bowker, Joyce’s biographer, agreed: “Joyce’s problem with the UK printers related to the fact that here in those days printers were as much at risk of prosecution on charges of publishing obscenities as were publishers, and would simply refuse to print them.