(1) Reactivity differences in weight among the CEA preparations were, however, observed in all but one assay kit (D-ZYME CEA) in which all CEAs showed fairly homogeneous reactivity.
(2) Antigenic reactivities of 4 purified CEA preparations and 4 different CEA-related normal antigens (NCA from lungs, NCA-2 from meconium, and NFA-1 and NFA-2 from adult feces) were comparatively analyzed with seven commercially available EIA kits [Abbott CEA-EIA Monoclonal, D-ZYME CEA, Imzyne CEA, CEA MITSUI II, CEA Roche EIA, Immunoball-CEA (N), and Glaozyme CEA].
(3) The investigation required the use of the Staphylo-Zyme P.B.I.
(4) However, one assay kit (D-ZYME CEA), whose reactivity to different CEAs was fairly homogeneous, could discriminate CEAs from NCA-2 and NFA-2.
(5) In the study herein reported, the hydrolysis was accelerated and controlled by using the following enzymes: papain, HT proteolytic, and Brew (N) zyme.
(6) It was found that the temperature optimum for endo-beta-glucanase of all preparations was 50 degrees, for beta-glucanase and pentosanase--40 degrees, for cellobiase--70 degrees C. The thermal stability of endo-beta-glucanase from amylosubtilin G10x and brew-n-zyme in the aqueous solution was higher than that from ditorosemin Px and P10x.
(7) Multiple molecular forms contribute to various types of enzyme heterogeneity: "Iso(en)-zymes" and "Allozymes" (enzyme variants) are of genetic origin whereas "Metazymes" represent secondary modifications of epigenetic nature.
(8) The cell specificity detected by these antibodies can be removed only by an alpha-N-acetylgalactosaminidase (A-zyme) but not by an alpha-galactosidase (B-zyme).
(9) The temperature optimum and thermal stability of endo-beta-glucanase, beta-glucanase, pentosanase and cellobiase from the enzymic preparations used in the brewing industry to treat high amounts of unmalted barley--citorosemin Px, citorosemin P10x, amylosubtilin G10x and brew-n-zyme--were measured.
(10) The other glycolytic zymes having high particulate activity, aldolase, glucosephosphate isomerase, phosphofructokinase, glyceraldehyde-phosphate dehydrogenase, pyruvate kinase and lactate dehydrogenase, showed enrichment in fractions containing synaptosomal membranes, i.e.
(11) The of 3H-DFP to label and characterize serine zymes in the culture fluid from PMA-treated cells further indicated that PA is the serine protease responsible for the morphological changes.
(12) A colorimetric method for the detection of 19 constitutively expressed enzymes under in situ conditions of pressure and temperature has been devised, using a simple modification of the commercially available API ZYME enzyme assay kit.
(13) Therefore rifampin preferentially affects norantipyrine or desmethyl- and 3-hydroxydiazepam metabolic formation, suggesting induction of different (iso)zymes of cytochrome P-450.
(14) As results indicated, the best hydrolysate was obtained with Brew (N) zyme at 50 degrees C and 8.30 hours.
(15) Moreover, papain treatment of normal group B cells not previously agglutinable with these antibodies, now causes these cells to become reactive, and this specificity, too, is removed only by A-zyme.
(16) Frozen sections of hamster ovaries were stained immunohistochemically for EGF using a Zymed kit.