(1) In addition to oncogenes, the transferred DNA contains genes that direct the synthesis and exudation of opines, which are used as nutrients by the bacteria.
(2) The pH gradient measured with dimethyloxazolidine-2,4-dione and acetylsalicylic acid was very small in both bacteria at a high pH above 8, and was not affected significantly by the addition of CCCP.
(3) The causative organisms included viruses, fungi, and bacteria of both high and low pathogenicity.
(4) Anaerobes, in particular Bacteroides spp., are the predominant bacteria present in mixed intra-abdominal infections, yet their critical importance in the pathogenicity of these infections is not clearly defined.
(5) During the digestion of these radiolabeled bacteria, murine bone marrow macrophages produced low-molecular-weight substances that coeluted chromatographically with the radioactive cell wall marker.
(6) The authors conclude that H. pylori alone causes little or no effect on an intact gastric mucosa in the rat, that either intact organisms or bacteria-free filtrates cause similar prolongation and delayed healing of pre-existing ulcers with active chronic inflammation, and that the presence of predisposing factors leading to disruption of gastric mucosal integrity may be required for the H. pylori enhancement of inflammation and tissue damage in the stomach.
(7) Thirty-two strains of pectin-fermenting rumen bacteria were isolated from bovine rumen contents in a rumen fluid medium which contained pectin as the only added energy source.
(8) This capacity is expressed during incubation of the bacteria with the substrate and needs a source of carbon and other energy metabolites.
(9) Preincubation of the bacteria at 56 degrees C for 30 minutes and ultraviolet irradiation resulted in a noticeable decrease in adherence.
(10) An sdh-specific transcript of about 3,450 nucleotides was detected in vegetative bacteria.
(11) The authors present the first results on the utilization of fish infusion (IFP) as a basic medium for the cultivation of bacteria.
(12) Phospholipid changes occurring at later stages in the lytic cycle of infected bacteria are more prominent than those at earlier time intervals.
(13) The most commonly encountered organisms were aerobic bacteria (91%), anaerobes (74%), and fungi (48%).
(14) Resistance to antibiotics have been detected in food poisoning bacteria, namely Salmonella typhimurium, Staphylococcus aureus and Clostridium perfringens.
(15) Bacteria can stop or lessen antibodies synthesis process.
(16) Among the agents triggering such an infection Chlamydia (30.9% of the cases of non-gonorrhoic urethritis), as well as mycoplasma, ureaplasma, anaerobic bacteria and herpes simplex viruses have gained particular significance.
(17) Mu does not grow lytically in or kill him bacteria but can lysogenize such hosts.
(18) Tunnel-like formations at different depths of the oral epithelium contained higher numbers of bacteria than those seen on the adjacent oral surface.
(19) The cells were taken from cultures in low-density balanced exponential growth, and the experiments were performed quickly so that the bacteria were in a uniform physiological state at the time of measurement.
(20) Subgingival plaque was sampled and the presence or absence of the above mentioned bacteria assessed with BANA reagent cards (Perio Scan).
Plasmid
Definition:
(n.) A piece of DNA, usually circular, functioning as part of the genetic material of a cell, not integrated with the chromosome and replicating independently of the chromosome, but transferred, like the chromosome, to subsequent generations. In bacteria, plasmids often carry the genes for antibiotic resistance; they are exploited in genetic engineering as the vehicles for introduction of extraneous DNA into cells, to alter the genetic makeup of the cell. The cells thus altered may produce desirable proteins which are extracted and used; in the case of genetically altered plant cells, the altered cells may grow into complete plants with changed properties, as for example, increased resistance to disease.
Example Sentences:
(1) When micF was cloned into a high-copy-number plasmid it repressed ompF gene expression, whereas when cloned into a low-copy-number plasmid it did not.
(2) These eight large plasmids had indistinguishable EcoRI restriction patterns.
(3) The combined analysis of pathogenesis and genetics associated with the salmonella virulence plasmids may identify new systems of bacterial virulence and the genetic basis for this virulence.
(4) On removal of selective pressure, the His+ phenotype was lost more readily than the Ura+ Trp+ markers, with a corresponding decrease in plasmid copy number.
(5) The data on mapping the episomal plasmid integration sites in yeast chromosomes I, III, IV, V, VII, XV are presented.
(6) A beta-adrenergic receptor cDNA cloned into a eukaryotic expression vector reliably induces high levels of beta-adrenergic receptor expression in 2-12% of COS cell colonies transfected with this plasmid after experimental conditions are optimized.
(7) By hybridization studies, three plasmids in two forms (open circular and supercoiled) were detected in the strain A24.
(8) It should be noted that about a half of the plasmids (11 out of 21) belonged to the incompatibility group P-7 which up to the present time was conditional, since was represented by a single plasmid Rms 148.
(9) Since the plasmid-cured strains did not contain DNA sequences homologous to plasmid DNA, the gene for the free-inclusion protein must be encoded in the chromosome.
(10) A total of 28 cell lines were selected for Geneticin - resistance and inoculated into the footpads of syngeneic animals following co-transfection with pSV2neo and genomic DNA, or transfection with plasmid constructs containing neo and the activated Ha-ras oncogene.
(11) Since resistance is mainly mediated by R plasmids, we undertook to investigate the characteristics of R plasmid-determined beta-lactamase in 6 Gram-negative rods.
(12) The 6.6-kb DNA Bam HI fragment containing the resistance gene was cloned with the plasmid vector pIJ699.
(13) Only one E. coli strain, containing two plasmids that encode endo-pectate lyases, exo-pectate lyase, and endo-polygalacturonase, caused limited maceration.
(14) Plasmids containing the inverted repeat alone bound ER, though less efficiently than did plasmids containing the entire sequence.
(15) The plasmid pMucAMucB, constructed from the Haemophilus influenzae vector pDM2, and a similar plasmid, constructed from pBR322, increased the survival after UV irradiation of Escherichia coli AB1157 with the umu-36 mutation and also caused UV-induced mutation in the E. coli strain.
(16) The 105 000 X g supernatant of the reaction mixture, which contained more than 85% of the thiobarbituric acid-reactive substances, did not inactivate the plasmid DNA.
(17) The remainder of the plasmid appeared to be associated with five positioned nucleosomes and two nonnucleosomal, partially protected regions on the bulk of the molecules.
(18) The fifth plasmid contains sequences which are repeated in the yeast genome, but it is not known whether any or all of the ribosomal protein gene on this clone contains repetitive DNA.
(19) Plasmid profiling was used to distinguish strains of lactobacilli inhabiting the digestive tract of piglets and the feces of sows.
(20) One mutant, BS260, was completely noninvasive on HeLa cells and mapped to a region on the 220-kb virulence plasmid in which we had previously localized several avirulent temperature-regulated operon fusions (A.E.