(1) We fused that region of the subtilisin gene, (apr[BamP]), which encodes the signal sequence and pro region, to the mature gene sequence (spa) for a heterologous protein (staphylococcal protein A).
(2) The Bacillus amyloliquefaciens levansucrase gene (sacB[BamP]) was engineered in such a way that a heterologous gene could be inserted between the second and third codon of the mature levansucrase.
(3) The nucleotide sequence preferences of the DNA interstrand cross-linking agents dehydroretronecine diacetate (DHRA), 2,3-bis(acetoxymethyl)-1-methylpyrrole (BAMP), dehydromonocrotaline, and dehydroretrorsine were studied by using synthetic DNA duplex fragments and polyacrylamide gel electrophoresis (PAGE).
(4) The concentrations of three membrane-associated proteins (MAP), beta 2-microglobulin (beta 2M), secretory component (SC) and bovine-associated mucoprotein (BAMP) as well as albumin were measured in each aliquot to determine if in vivo proteolysis of milk elements could explain the origin of these MAP in milk.
(5) The sacB[BamP] gene cloned in a multicopy plasmid is induced by sucrose in B. subtilis.
(6) Furthermore, the impact of flanking sequence on the efficiency of interstrand cross-linking at 5'-CG was reduced for BAMP, with 5'-TCGA and 5'-GCGC being equally efficiently cross-linked.
(7) Data revealed that all BAMP in milk can be accounted for by in vivo proteolytic degradation of MFG while most beta 2M is derived by similar degradation, from cellular elements in milk, presumably monocytes.
(8) For DHRA and BAMP interstrand cross-linked DNA duplexes, PAGE analysis of iron(II)-EDTA fragmentation reactions revealed the interstrand cross-links to be deoxyguanosine to deoxyguanosine (dG-to-dG), again analogous to DNA cross-links caused by MC.
(9) The second fusion (apr-bpr2) joined the end of the apr[BamP] signal peptide coding sequence to the mature bpr resulting in a hybrid signal processing site ala-lys.
(10) The gene encoding levansucrase (LVS) from Bacillus amyloliquefaciens (sacB[BamP]) was isolated, sequenced and expressed in Bacillus subtilis.
(11) The first gene fusion (apr-bpr1) contained the apr[BamP] signal peptide coding region fused to mature bpr through a linker coded 3-amino acid region and retained the signal processing site ala-ala of the alkaline protease.
(12) Analysis of the nucleotide sequence of sacB[BamP] reveals extensive homology with that of the B. subtilis LVS-encoding gene in the promoter and coding region.
(13) Two different hybrid genes were constructed which fuse the Bacillus amyloliquefaciens alkaline protease gene (apr[BamP]) promoter and signal peptide coding region to a synthetic bpr gene coding for the mature bovine pancreatic RNase A.
Bump
Definition:
(v. t.) To strike, as with or against anything large or solid; to thump; as, to bump the head against a wall.
(v. i.) To come in violent contact with something; to thump.
(n.) A thump; a heavy blow.
(n.) A swelling or prominence, resulting from a bump or blow; a protuberance.
(n.) One of the protuberances on the cranium which are associated with distinct faculties or affections of the mind; as, the bump of "veneration;" the bump of "acquisitiveness."
(n.) The act of striking the stern of the boat in advance with the prow of the boat following.
(v. i.) To make a loud, heavy, or hollow noise, as the bittern; to boom.
(n.) The noise made by the bittern.
Example Sentences:
(1) Believe it or not, I still bump into people who have a good word to say about George Osborne .
(2) He, along with the world's policymakers, will be hoping that the waves in emerging markets created by his final act will prove to be a bump on the road to global recovery, and not the beginning of a fresh crisis.
(3) Shot noise analysis indicated that a combination of intense light and La3+ caused a large (down to zero) reduction in the rate of occurrence of the quantal responses to single photons (quantum bumps) which sum to produce the photoreceptor potential.
(4) 1.57pm BST Lap 36: Punchy stuff from Jules Bianchi up to 13th, literally bumping his way through Kobayashi on the inside.
(5) The immigration minister, Scott Morrison, headed to Papua New Guinea on Friday to discuss Manus Island violence and refugee resettlement and to iron out what the PNG foreign minister, Rimbink Pato, describes as “bumps” in an asylum policy partnership that is still intact.
(6) When Matt Slater went swimming with his dog Mango in a Cornish estuary this month, he bumped into a barrel jellyfish.
(7) This year, on the first day, I bumped into a fellow market regular who was hawking a DVD title (no longer a badge of shame).
(8) But Gates’s decision to “bump off from art” and live “in the sphere of dirt, the dirty, the stuff that we think is in the ground” was revelatory, leading to invitations to Davos and a TED Talk, where he talked about how he revived a neighborhood with imagination and hard graft .
(9) Earlier that year he appeared to bump into the same opponent after losing to him.
(10) By using a temperature-sensitive allele, we have found that that norpA mutation has little or no effect on either the rhodopsin-metarhodopsin transition or the machinery of quantum bump production.
(11) It is now surely José Mourinho’s Premier League title to lose after Loïc Rémy ironed out a bump on the road for Chelsea with the late winner.
(12) The Cowboys, meanwhile, move to 7-3 and are back on the play-off road after a couple of recent bumps.
(13) From time to time I'd bump into Amy she had good banter so we could chat a bit and have a laugh, she was a character but that world was riddled with half-cut, doped-up chancers, I was one of them, even in early recovery I was kept afloat only by clinging to the bodies of strangers so Winehouse, but for her gentle quirks didn't especially register.
(14) If anionic production of quantum bumps in Limulus photoreceptors is mediated by changes in cyclic nucleotides, then the electrophysiological response of Limulus photoreceptors to tungstate may indicate a role for phosphodiesterase rather than adenylate cyclase in mediating light-induced cyclic nucleotide alterations in this cell.
(15) I have weekly massages to iron out all the bumps and grumbles in my legs.
(16) "There will always be bumps in the road … It's a relationship that can withstand those," the US official said.
(17) Mardi Gras is one of the best, friendliest, loveliest events that we have in our city A big smile for greeting people that you know, because you’ll bump into them everywhere.
(18) Similarly, the Ernst & Young Item Club forecasting group recently warned that Britain faces a painful and prolonged "VW-shaped recovery" as the economy "bumps along the bottom", held back by weaker consumer spending and government cost-cutting.
(19) The usual Monday lineup of Australian Story, Four Corners, Media Watch and Q&A were either shunted to ABC2 or bumped to next week as Canberra’s broadcasting team took over.
(20) The cellular mechanism for reducing the rate of spontaneous quantum bumps is not known.