(1) We fused that region of the subtilisin gene, (apr[BamP]), which encodes the signal sequence and pro region, to the mature gene sequence (spa) for a heterologous protein (staphylococcal protein A).
(2) The Bacillus amyloliquefaciens levansucrase gene (sacB[BamP]) was engineered in such a way that a heterologous gene could be inserted between the second and third codon of the mature levansucrase.
(3) The nucleotide sequence preferences of the DNA interstrand cross-linking agents dehydroretronecine diacetate (DHRA), 2,3-bis(acetoxymethyl)-1-methylpyrrole (BAMP), dehydromonocrotaline, and dehydroretrorsine were studied by using synthetic DNA duplex fragments and polyacrylamide gel electrophoresis (PAGE).
(4) The concentrations of three membrane-associated proteins (MAP), beta 2-microglobulin (beta 2M), secretory component (SC) and bovine-associated mucoprotein (BAMP) as well as albumin were measured in each aliquot to determine if in vivo proteolysis of milk elements could explain the origin of these MAP in milk.
(5) The sacB[BamP] gene cloned in a multicopy plasmid is induced by sucrose in B. subtilis.
(6) Furthermore, the impact of flanking sequence on the efficiency of interstrand cross-linking at 5'-CG was reduced for BAMP, with 5'-TCGA and 5'-GCGC being equally efficiently cross-linked.
(7) Data revealed that all BAMP in milk can be accounted for by in vivo proteolytic degradation of MFG while most beta 2M is derived by similar degradation, from cellular elements in milk, presumably monocytes.
(8) For DHRA and BAMP interstrand cross-linked DNA duplexes, PAGE analysis of iron(II)-EDTA fragmentation reactions revealed the interstrand cross-links to be deoxyguanosine to deoxyguanosine (dG-to-dG), again analogous to DNA cross-links caused by MC.
(9) The second fusion (apr-bpr2) joined the end of the apr[BamP] signal peptide coding sequence to the mature bpr resulting in a hybrid signal processing site ala-lys.
(10) The gene encoding levansucrase (LVS) from Bacillus amyloliquefaciens (sacB[BamP]) was isolated, sequenced and expressed in Bacillus subtilis.
(11) The first gene fusion (apr-bpr1) contained the apr[BamP] signal peptide coding region fused to mature bpr through a linker coded 3-amino acid region and retained the signal processing site ala-ala of the alkaline protease.
(12) Analysis of the nucleotide sequence of sacB[BamP] reveals extensive homology with that of the B. subtilis LVS-encoding gene in the promoter and coding region.
(13) Two different hybrid genes were constructed which fuse the Bacillus amyloliquefaciens alkaline protease gene (apr[BamP]) promoter and signal peptide coding region to a synthetic bpr gene coding for the mature bovine pancreatic RNase A.
Damp
Definition:
(n.) Moisture; humidity; fog; fogginess; vapor.
(n.) Dejection; depression; cloud of the mind.
(n.) A gaseous product, formed in coal mines, old wells, pints, etc.
(superl.) Being in a state between dry and wet; moderately wet; moist; humid.
(superl.) Dejected; depressed; sunk.
(n.) To render damp; to moisten; to make humid, or moderately wet; to dampen; as, to damp cloth.
(n.) To put out, as fire; to depress or deject; to deaden; to cloud; to check or restrain, as action or vigor; to make dull; to weaken; to discourage.
Example Sentences:
(1) Of great influence on the results of measurements are preparation and registration (warm-up-time, amplification, closeness of pressure-system, unhurt catheters), factors relating to equipment and methods (air-bubbles in pressure-system, damping by filters, continuous infusion of the micro-catheter, level of zero-pressure), factors which occur during intravital measurement (pressure-drop along the arteria pulmonalis, influence of normal breathing, great intrapleural pressure changes, pressure damping in the catheter by thrombosis and external disturbances) and last not least positive and negative acceleration forces, which influence the diastolic and systolic pulmonary artery pressure.
(2) These two quantities were used to calculate the damping factor.
(3) Children still received an education, it was just that increasing numbers did so in damp and dilapidated buildings.
(4) Bloody odd combination but those Orange Foam Headphones would blast those magnificent records into my developing brain over and over again" chernypyos – Björk's Human Behavior and Sinead O'Connor's Fire On Babylon: "bjork's 'human behavior' and sinead o'connor's "fire on babylon" oddly stick in my head from that one evening walking in the woods, breathing the damp air, and feeling pleasantly invisible" Pyromancer – REM – Automatic for the People Blood Sugar Sex Magic Pearl Jam - Vs RATM's first album Portishead Maxinquaye by Tricky Manic Street Preachers – Gold Against the Soul Smashing Pumpkins, Siamese Dream "I used to go to the local library and take out a CD (50p for 3 weeks!
(5) The frequency distribution of the natural, damped and resonance frequencies of the second-order activities was found to show one, two or three modes in each frequency range of the delta, theta and beta rhythms in ninety normal adult EEGs.
(6) vittatus eggs laid on damp mud were placed in dry rockpools for 10 weeks and kept dry for a further 6 weeks in the laboratory.
(7) It’s a damp squib, a bit of a nothing result,” a leading energy analyst said of a report that is widely expected to endorse provisional findings released in March , and recommend price controls on prepayment meters and setting up a customer database to help rival suppliers target customers stuck on expensive default tariffs.
(8) Performing this reaction in H2 18O gave [18O]dps(ATCGTC) (a pentamer containing an 18O-labeled 5'-phosphorothioate) which was converted to [18O]dAMPS with nuclease P1.
(9) The damping time of the acceleration wave also had a negative correlation with age.
(10) The former time-pattern displays a fast rise and an exponential decay, while the latter exhibits a damped sine wave.
(11) Studies of activity levels indicate that tsetse flies should aggregate in damp situations where the activity levels is minimal, whereas in practice the flies are distributed throughout the whole of gradient.
(12) In response to dynamic forces the platform showed a resononance frequency of about 50 Hz, with a damping factor of 0.15.
(13) 4-DAMP (4-diphenylacetoxy N-methylpiperidine; a M3-receptor antagonist), strongly inhibited the ACh-induced constrictions.
(14) 4-Diphenylacetoxy-1:1-dimethyl cyclohexane (carbo-4-DAMP) is the carbon analogue of 4-diphenylacetoxy-N-methylpiperidine (4-DAMP) methiodide.
(15) Posture control performance was quantified in three variables (swiftness, stiffness, damping).
(16) The first 2 ms of these transients were described by a linear model in which the fibre is regarded as a rod composed of infinitesimally small, identical segments containing a mass, one undamped elastic element and in the case of relaxed fibres two damped elastic elements in series, or in the case of activated fibres three such elastic elements in series.
(17) This would implicate the existence of a dynamic control through which the sensory system may register and damp the pressure response.
(18) Trying to solve those problems by closing the borders is like trying to deal with rising damp by bolting your front door Trying to solve those problems by closing the borders is like trying to deal with rising damp by bolting your front door.
(19) Response is dependent upon the external impulse plus system inertia, damping and stiffness.
(20) In humans, ethanol produces a slow damped oscillation in the steady electrical potential of the eye, which is generated primarily by the RPE.