(1) We fused that region of the subtilisin gene, (apr[BamP]), which encodes the signal sequence and pro region, to the mature gene sequence (spa) for a heterologous protein (staphylococcal protein A).
(2) The Bacillus amyloliquefaciens levansucrase gene (sacB[BamP]) was engineered in such a way that a heterologous gene could be inserted between the second and third codon of the mature levansucrase.
(3) The nucleotide sequence preferences of the DNA interstrand cross-linking agents dehydroretronecine diacetate (DHRA), 2,3-bis(acetoxymethyl)-1-methylpyrrole (BAMP), dehydromonocrotaline, and dehydroretrorsine were studied by using synthetic DNA duplex fragments and polyacrylamide gel electrophoresis (PAGE).
(4) The concentrations of three membrane-associated proteins (MAP), beta 2-microglobulin (beta 2M), secretory component (SC) and bovine-associated mucoprotein (BAMP) as well as albumin were measured in each aliquot to determine if in vivo proteolysis of milk elements could explain the origin of these MAP in milk.
(5) The sacB[BamP] gene cloned in a multicopy plasmid is induced by sucrose in B. subtilis.
(6) Furthermore, the impact of flanking sequence on the efficiency of interstrand cross-linking at 5'-CG was reduced for BAMP, with 5'-TCGA and 5'-GCGC being equally efficiently cross-linked.
(7) Data revealed that all BAMP in milk can be accounted for by in vivo proteolytic degradation of MFG while most beta 2M is derived by similar degradation, from cellular elements in milk, presumably monocytes.
(8) For DHRA and BAMP interstrand cross-linked DNA duplexes, PAGE analysis of iron(II)-EDTA fragmentation reactions revealed the interstrand cross-links to be deoxyguanosine to deoxyguanosine (dG-to-dG), again analogous to DNA cross-links caused by MC.
(9) The second fusion (apr-bpr2) joined the end of the apr[BamP] signal peptide coding sequence to the mature bpr resulting in a hybrid signal processing site ala-lys.
(10) The gene encoding levansucrase (LVS) from Bacillus amyloliquefaciens (sacB[BamP]) was isolated, sequenced and expressed in Bacillus subtilis.
(11) The first gene fusion (apr-bpr1) contained the apr[BamP] signal peptide coding region fused to mature bpr through a linker coded 3-amino acid region and retained the signal processing site ala-ala of the alkaline protease.
(12) Analysis of the nucleotide sequence of sacB[BamP] reveals extensive homology with that of the B. subtilis LVS-encoding gene in the promoter and coding region.
(13) Two different hybrid genes were constructed which fuse the Bacillus amyloliquefaciens alkaline protease gene (apr[BamP]) promoter and signal peptide coding region to a synthetic bpr gene coding for the mature bovine pancreatic RNase A.
Imbecile
Definition:
(a.) Destitute of strength, whether of body or mind; feeble; impotent; esp., mentally wea; feeble-minded; as, hospitals for the imbecile and insane.
(n.) One destitute of strength; esp., one of feeble mind.
(v. t.) To weaken; to make imbecile; as, to imbecile men's courage.
Example Sentences:
(1) Infantile delivery also frequently serves to take the curse off self-publicity; sleight of hand for those who find "my programme is on BBC2 tonight" too presumptuous and exposing, and prefer to cower behind the low-status imbecility of "I done rote a fingy for da tellybox!"
(2) By this shape of holidays the partical sphere of the process of training and education, namely the qualification of those oligophren ones in spending an ingenious leisure, should be noticed and contributed to educating those imbecile boys and girls, who are participating their holidays in a camp for their "relative independence*.
(3) Fifty-six patients with cerebral atherosclerosis and epileptiform symptomatology presented an organic defect with signs of lacunar imbecility and atherosclerotic asthenia.
(4) Report on a 5 year old girl with the caracteristic features of the partial trisomy of the short arm of a chromosome no.4: short stature, microcephaly, hydrocephaly, enophthalmus, bulbous nose, deep set malformed ears, hypertrichosis, brachydactyly, hypoplastic ribs, abnormal EEG, imbecility.
(5) "This is imbecilic," said Jean-Yves Oussedik, a historian, puffing his pipe outside the literary cafe Les Deux Magots.
(6) Target London , a folio of 18 posters, bleakly satirised the Thatcher government’s Protect and Survive nuclear attack directives; the critic Richard Cork described the series as the “most hard-hitting attack on government imbecility”.
(7) And there I was, week after week, paid a pittance to jeer at the Smith regime's imbecilities.
(8) It's time to address the public as competent grown-ups and not as imbeciles.
(9) As a late sequelae, there was one patient with intrahepatic block and portal hypertension and one with encephalopathy and imbecility.
(10) Treatment under general anesthesia is inevitably indicated in imbeciles, the feeble-minded, spastics, epileptics, and sometimes in mongoloids.
(11) But before he was a candidate, he was just a visible idiot, and Jon Stewart’s version of him as a knuckle-dragging Queens County imbecile has given us tremendous joy over the years.
(12) The disease had not been diagnosed during life despite imbecility since early childhood and the presence of guiding peripheral symptoms in the form of Pringle's disease.
(13) Meanwhile, because we no longer understand anything unless it is filtered through the prism of the Premier League, various newspapers have already dubbed May's poll " the Wags election " – a classification that underscores the almost infinite creativity of the British media, which have apparently now given up so emphatically that they are content to shoehorn absolutely all human experience into one of four or five pop-cultural tropes, the easier for the voters it apparently regards as imbeciles to understand.
(14) In broadcast interviews, ministers carefully dodge the delivery of any information at all; they would rather sound imbecilic, as if they understood very little and knew even less, than run the risk of having said anything of import.
(15) The differential-diagnostic criteria show the difference of the episodic psychoses of imbeciles from schizophrenias (grafted schizophrenias).
(16) The experience gathered thus far shows that the method presented by the author in his present paper enables the capacity and development of imbecile and abnormality feeble children and juveniles to be diagnosed.
(17) When some highly debile, or imbecile and idiotic children and adolescents refuse to cooperate during the stomatological attendance, the pedopsychiatric consultation fails.
(18) Yet social media is the last remaining British arena in which social mobility flourishes, where imbecilic irrelevances are fast-tracked to positions of extraordinary power by whichever MP or university professor or serious campaigner has decided to give their bile a platform on the news.
(19) Statistically significant differences were established in the values of integrative mark estimations of patients with pronounced debility, of those with mild, medium and profound imbecility.
(20) Patients with pronounced tetrapareses and contractures in all the joints, grave hyperkinesias in all the four extremities, and imbecility were classed with disability group I: those with pronounced para-, hemi-, and tetrapareses, extensive hyperkinesias, combination of the motor disorders with debility were placed into disability group II.