(1) We fused that region of the subtilisin gene, (apr[BamP]), which encodes the signal sequence and pro region, to the mature gene sequence (spa) for a heterologous protein (staphylococcal protein A).
(2) The Bacillus amyloliquefaciens levansucrase gene (sacB[BamP]) was engineered in such a way that a heterologous gene could be inserted between the second and third codon of the mature levansucrase.
(3) The nucleotide sequence preferences of the DNA interstrand cross-linking agents dehydroretronecine diacetate (DHRA), 2,3-bis(acetoxymethyl)-1-methylpyrrole (BAMP), dehydromonocrotaline, and dehydroretrorsine were studied by using synthetic DNA duplex fragments and polyacrylamide gel electrophoresis (PAGE).
(4) The concentrations of three membrane-associated proteins (MAP), beta 2-microglobulin (beta 2M), secretory component (SC) and bovine-associated mucoprotein (BAMP) as well as albumin were measured in each aliquot to determine if in vivo proteolysis of milk elements could explain the origin of these MAP in milk.
(5) The sacB[BamP] gene cloned in a multicopy plasmid is induced by sucrose in B. subtilis.
(6) Furthermore, the impact of flanking sequence on the efficiency of interstrand cross-linking at 5'-CG was reduced for BAMP, with 5'-TCGA and 5'-GCGC being equally efficiently cross-linked.
(7) Data revealed that all BAMP in milk can be accounted for by in vivo proteolytic degradation of MFG while most beta 2M is derived by similar degradation, from cellular elements in milk, presumably monocytes.
(8) For DHRA and BAMP interstrand cross-linked DNA duplexes, PAGE analysis of iron(II)-EDTA fragmentation reactions revealed the interstrand cross-links to be deoxyguanosine to deoxyguanosine (dG-to-dG), again analogous to DNA cross-links caused by MC.
(9) The second fusion (apr-bpr2) joined the end of the apr[BamP] signal peptide coding sequence to the mature bpr resulting in a hybrid signal processing site ala-lys.
(10) The gene encoding levansucrase (LVS) from Bacillus amyloliquefaciens (sacB[BamP]) was isolated, sequenced and expressed in Bacillus subtilis.
(11) The first gene fusion (apr-bpr1) contained the apr[BamP] signal peptide coding region fused to mature bpr through a linker coded 3-amino acid region and retained the signal processing site ala-ala of the alkaline protease.
(12) Analysis of the nucleotide sequence of sacB[BamP] reveals extensive homology with that of the B. subtilis LVS-encoding gene in the promoter and coding region.
(13) Two different hybrid genes were constructed which fuse the Bacillus amyloliquefaciens alkaline protease gene (apr[BamP]) promoter and signal peptide coding region to a synthetic bpr gene coding for the mature bovine pancreatic RNase A.
Tamp
Definition:
(v. t.) In blasting, to plug up with clay, earth, dry sand, sod, or other material, as a hole bored in a rock, in order to prevent the force of the explosion from being misdirected.
(v. t.) To drive in or down by frequent gentle strokes; as, to tamp earth so as to make a smooth place.
Example Sentences:
(1) The results support the use by physical therapists of two parallel versions of the TAMP to describe the motor performance of adults and school-aged children with physical disabilities.
(2) The Tufts Assessment of Motor Performance (TAMP) was administered to 69 children (ages 6-18 years, X = 12.1, SD = 3.9) and 137 adults (ages 19-83 years, X = 46.7, SD = 20.0) with neurological and musculoskeletal impairments.
(3) The Tamp cells are found in the spleens of donor mice at 6 days after immunization with cryptococcal antigen, and they amplify the anticryptococcal DTH response when transferred to syngeneic recipients at the time of immunization of the recipients.
(4) At the same time, he tamped down speculation that US warplanes would strike Isis in Syria as well as Iraq.
(5) The cohesiveness of size fractions of acetylsalicylic acid and lactose alone and in various combinations has been determined by estimation of the rate of decrease in volume as a function of tamping under standard conditions.
(6) The structure of 2-BDB-TAMP was determined by UV, 1H NMR, and 13C NMR spectroscopy as well as by bromide and phosphorus analysis.
(7) The rate constant (kobs) for loss of ADP activation exhibits a nonlinear dependence on 2-BDB-TAMP concentration, suggesting a reversible binding of reagent (KR = 0.74 mM) prior to irreversible modification.
(8) Hillary Clinton’s mea culpa at the United Nations on Tuesday was supposed to tamp down the scandal over her use of a private email address as secretary of state.
(9) While Clinton’s campaign has long gone out of its way to tamp down expectations in Iowa, it is also worth noting that the closest analogue to her 2016 candidacy in modern times, that of then vice-president Al Gore in 2000, received more than 63% of the Iowan vote.
(10) At 1.2 mM 2-BDB-TAMP, kobs = 0.060 min-1 and is not affected by alpha-ketoglutarate or GTP, but is decreased to 0.020 min-1 by 5 mM NADH and to zero by 5 mM ADP.
(11) The NSA has been extraordinarily careful not to lose her support, even as she has bristled against its spying on foreign leaders and tamped down internal committee challenges to the NSA.
(12) The California Correctional Peace Officers Association, a union representing 27,400 members, has defended their right to select inmates for solitary confinement and to determine the punishment's duration, saying it helps to tamp down violence.
(13) Cash register operation, regardless of register system, caused static load (mean) in the thoracic erector spinae at 4.1-6.2 TAMP% (Time-Averaged Myoelectrical Potential), in the infraspinatus at 2.9-5.8 TAMP%, and in the trapezius at 2.8-3.8 TAMP%.
(14) The preoperative recognition of the CA I-IV and NC III fracture types proved essential to the successful resection of five Type I, five Type II, 36 Type III, and ten Type IV fragments through extended laminotomies, hemilaminectomies, and laminectomies with the down biting curette, tamp, and mallet technique.
(15) Two of the compounds, L-bis (1-thio-2-amino-4-methylpentane) dihydrochloride (TAMP) and L-bis (1-thio-2-amino-3-phenylpropane) dihydrochloride (TAPP), caused significant inhibition.
(16) The final product was generated by reaction of TAMP with 1,4-dibromobutanedione.
(17) The cancellous and cortical bone from the window is then tamped in behind the patella tendon, buttressing the tendon, and permitting the knee a full unguarded range of motion on the operating table.
(18) An evaluation of seven compaction parameters revealed that all tamping stations and all piston positions within a station, with the exception of station #1, contribute equally to plug formation.
(19) We have to lift them up and not deny them or try to tamp them down, but to try to understand them and figure out how to make more progress.” “We need to recognise that the situation in Ferguson speaks to the broader problems that we still face as a nation,” he added.
(20) In contrast, when Ts1 cells were given at the time of adoptive transfer of Tamp cells, the recipients displayed amplified DTH responses, indicating that Ts1 cells do not affect the Tamp cells' function once the Tamp cells have been produced.