What's the difference between biuret and urea?

Biuret


Definition:

  • (n.) A white, crystalline, nitrogenous substance, C2O2N3H5, formed by heating urea. It is intermediate between urea and cyanuric acid.

Example Sentences:

  • (1) Each fraction (Cohn Fractions II, III, IV, and V) was assigned a protein concentration value that was determined by the biuret method, which we calibrated with purified monomer of human serum albumin.
  • (2) In order to investigate the consequences of presence of aggregates in human albumin standards, pools of monomer, dimer and polymer albumin were prepared and quantitated by three total-protein methods (biuret, Folin-Lowry and spectrophotometry at 279 nm) and by four different albumin methods (dye-binding by bromcresol green, electroimmunoassay, radial immunodiffusion and automated immunoprecipitation).
  • (3) Conscious Hartley guinea-pigs were sensitized with trimellitic anhydride (TMA) or trimeric hexamethylene diisocyanate biuret (Des-N) by repeated intradermal injections or by inhalation exposure.
  • (4) Micro-Kjeldahl and biuret techniques were also compared with mature seeds of a normal and a genetically-improved bean cultivar.
  • (5) We have studied the standardization of total serum protein assay with the biuret reaction.
  • (6) It is inexpensive, easily available, and exhibits the best linearity in the biuret reaction.
  • (7) The proteinuria of 21 samples of urine has been determined by the DOETSCH method and by the biuret method after protein precipitation by perchloric, trichloracetic and phosphotungstic acid.
  • (8) The biuret test with bilirubin-free protein yields results compatible with those obtained by nonphotometric techniques not involving bilirubin effects.
  • (9) The content of total protein and immunoglobulins in the breast-milk has been determined by the biuret-method and radial immunodiffusion.
  • (10) Cyanuric acid (1 mol) was converted stoichiometrically into 1.0 mol of CO2 and 1.1 mol of biuret, which was conclusively identified.
  • (11) Unlike the acetate or biuret type Cu(II) chelates these di-Schiff-base complexes had an identical reactivity compared to that of the intact enzyme.
  • (12) The mechanism of the biuret reaction is described and attention is drawn to the heterogeneity of the serum proteins and to the use of a certified albumin standard.
  • (13) The results were compared with those obtained from corresponding electrophoretic and biuret determinations, using Versatol as a standard-control throughout.
  • (14) Thermally and biologically unstable biuret and allophonate groups in this polyurethane, exposure of the polymer to high extrusion temperatures, and stresses created within the polymer also play key roles in the degradation process.
  • (15) The CBB method underestimated the protein concentration predicted by the biuret method by as much as eightfold in specimens with low proportions of albumin.
  • (16) A method was proposed which could estimate more accurately the fiber components of animal foods by measuring the "nondigestible protein" of the fiber sample of the fiber sample by the Biuret colorimetric method, instead of the Kjeldahl method, to avoid deducting the values for aminopolysaccharides.
  • (17) The urines were subsequently tested for protein by the following alternate methods: solubility of acid precipitate test, heat coagulation test, Upjohn modification of the sulfosalicylic acid test, biuret test, Albustix, and electrophoresis.
  • (18) In this study our previously described selected method, a biuret procedure with deproteinization, for the determination of spinal fluid protein is thoroughly discussed against the background of the results found with a number of Lowry modifications.
  • (19) The action of certain chemical mutagens such as N-nitroso methyl urea, N-nitroso ethyl urea, N-nitroso dimethyl urea and N-nitroso methyl biuret, as well as physical ones such as UV light on Escherichia coli producing penicillin acylase was studied.
  • (20) The filtrate was then assayed for total protein by the biuret-phenol method.

Urea


Definition:

  • (a.) A very soluble crystalline body which is the chief constituent of the urine in mammals and some other animals. It is also present in small quantity in blood, serous fluids, lymph, the liver, etc.

Example Sentences:

  • (1) These data suggest that the hybrid is formed by the same mechanism in the absence and presence of the urea step.
  • (2) The fourth cluster included the type strains of Actinobacillus lignieresii, A. equuli, A. pleuropneumoniae, A. suis, A. ureae, H. parahaemolyticus, H. parainfluenzae, H. paraphrohaemolyticus, H. ducreyi, and P. haemolytica.
  • (3) Thymus and spleen cells from such hypogammaglobulinaemic chickens were extracted with non-ionic detergents, acid urea, or combinations of urea and detergent, and the extracts were analysed for Ig by the inhibition assay.
  • (4) Triglyceride (Trigly) in female dogs, glutamic-pyruvic transaminase (GPT) and urea nitrogen (Urea-N) in male dogs tended to increase.
  • (5) The two main subunits present in rabbit skeletal tropomyosin, which have been named the alpha- and beta-chains, were separated by chromatography on CM-cellulose in urea at pH4.0.
  • (6) The temperature-activated 4 to 5 S EBP transformation is found to be highly reproducible without loss of [3H]estradiol-binding activity in a buffer containing an excess of [3H]estradiol, 40 mM Tris, 1 mM dithiothreitol, and 1 M urea at pH 7.4.
  • (7) The blockage of the tubular system by the calcium oxalate deposits leads to a temporary reversible increase in serum urea and serum creatinine.
  • (8) Urea was determined by means of diacetyl monoxim in the blood cells of 80 cockerels of the initial breed White Leghorn, commercial hybrid Primant.
  • (9) Urea decreased and valine increased in the TD medulla-pons.
  • (10) The complex was found to be unstable toward low values of pH and ionic strength, concentrations of urea exceeding 1 M, modifications of the cysteine residues, and fragmention in which the C terminal portions of either H3 or H4 are removed.
  • (11) The decrease in the amount of excreted urea by 85% was also accompanied by a reduced glomerular filtration rate (GFR).
  • (12) The modified osmium-urea method gave better results and was easier to perform than the collagenase method.
  • (13) Conversely, when fed NIH-07, both mouse genotypes had a 20-22% higher (p less than 0.003) serum urea nitogren and 2-3.5% higher erythrocyte count (p less than 0.001) and hemoglobin concentration (p less than 0.04) than when fed AIN-76A.
  • (14) (7) The first-order radical transformation rates are independent of the (initial) concentration of N3 or peptide and unaffected by urea (as a modifier of hydrogen bond structures).
  • (15) Treatment of the enzyme with acid and urea resulted in dissociation of the enzyme followed by loss of catalytic activity.
  • (16) 6) The subunits alpha and beta of bovine follitropin were obtained by incubation in acidic urea, the chains being then separated by anion exchange chromatography.
  • (17) There were no major differences in blood composition, apart from increases in blood urea N, as a result of N fertilization.
  • (18) In contrast to the facilitated water and urea transport systems in the red blood cell, these results suggest that the mechanism for water and urea transport in the platelet is primarily by diffusion through membrane phospholipid.
  • (19) A significant treatment X time interaction was found for plasma urea.
  • (20) The disc-gel patterns of the 8 m urea-soluble proteins showed that C. perfringens caused extensive proteolysis in pig muscle and a lesser extent of proteolysis in rabbit muscle.

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