(1) We have examined the recombination potential of BLIN-1 by characterizing several independently derived kappa-expressing subclones for DNA rearrangement and V kappa gene usage.
(2) The BLIN-1 cell line is, to our knowledge, the first defined human model for examining this critical developmental stage in human B cell ontogeny.
(3) By polymerase chain reaction analyses, we detected transcripts from rearranged V-J-C kappa genes as well as transcripts from germline J-C kappa and V kappa in BLIN-1 cells induced to rearrange the kappa locus.
(4) Our collective results indicate that: (a) BLIN-1 can be induced to rearrange the kappa locus, and this correlates with the expression of germline kappa locus transcripts that may play a role in activating or targeting gene rearrangement; and (b) active rearrangement and usage of V genes representing different kappa families suggest that, like in the mouse, repertoire diversification in humans occurs in the presence of a fixed heavy chain rearrangement.
(5) Binding studies using radiolabeled IL-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a KD of 150pM.
(6) Analysis of Ig gene rearrangement and Ig expression in a series of BLIN-1 subclones show that the cells spontaneously rearrange kappa light chain genes, leading to the differentiation of surface kappa-negative pre-B cells into surface kappa-positive B cells.
(7) In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate.
(8) The human pre-B acute lymphoblastic leukemia cell line, BLIN-1, has been previously shown to undergo kappa light chain rearrangement in vitro, making it a valuable resource for analyzing pre-B to B cell differentiation.
(9) This cell line, designated BLIN-1, was initially established in tissue culture medium containing low m.w.
(10) BLIN-1 cells have a 9p chromosomal abnormality, identical to the abnormality present in the leukemic blasts from the patient's original bone marrow aspirate.
(11) The immunologic phenotype of BLIN-1 is consistent with a cell arrested at the pre-B cell stage of development.
Blind
Definition:
(a.) Destitute of the sense of seeing, either by natural defect or by deprivation; without sight.
(a.) Not having the faculty of discernment; destitute of intellectual light; unable or unwilling to understand or judge; as, authors are blind to their own defects.
(a.) Having such a state or condition as a thing would have to a person who is blind; not well marked or easily discernible; hidden; unseen; concealed; as, a blind path; a blind ditch.
(a.) Involved; intricate; not easily followed or traced.
(a.) Having no openings for light or passage; as, a blind wall; open only at one end; as, a blind alley; a blind gut.
(a.) Unintelligible, or not easily intelligible; as, a blind passage in a book; illegible; as, blind writing.
(a.) Abortive; failing to produce flowers or fruit; as, blind buds; blind flowers.
(v. t.) To make blind; to deprive of sight or discernment.
(v. t.) To deprive partially of vision; to make vision difficult for and painful to; to dazzle.
(v. t.) To darken; to obscure to the eye or understanding; to conceal; to deceive.
(v. t.) To cover with a thin coating of sand and fine gravel; as a road newly paved, in order that the joints between the stones may be filled.
(n.) Something to hinder sight or keep out light; a screen; a cover; esp. a hinged screen or shutter for a window; a blinder for a horse.
(n.) Something to mislead the eye or the understanding, or to conceal some covert deed or design; a subterfuge.
(n.) A blindage. See Blindage.
(n.) A halting place.
(n.) Alt. of Blinde
Example Sentences:
(1) Clonazepam was added to the treatment of patients with poorly controlled epilepsy in a double-blind trial and an open trial.
(2) One rare case of blind-ending branch originating in the upper third of the ureter are described.
(3) We report the results of a double-blind, placebo-controlled trial of acitretin (Soriatane) in 15 patients with moderate to severe psoriasis.
(4) In a double-blind, crossover-designed study, 9 male subjects (age range: 18-25 years) received 25 mg orally, four times per day of either S or an identically-appearing placebo (P) 2 d prior to and during HA.
(5) In a randomized double-blind study, 40 patients with coronary heart disease received intravenously either 0.025 mg nitroglycerin or placebo.
(6) Eighty micrograms of the topically active parasympatholytic drug ipratropium were applied intranasally four times daily in 20 adults with perennial rhinitis and severe watery rhinorrhoea in a double-blind controlled cross-over trial.
(7) The effect of ipratropium bromide administered at two dosage levels, 40 and 80 mug, isoproterenol, 150 mug, and placebo using a metered dose inhaler was evaluated in ten adult patients with asthma in a double-blind, crossover study.
(8) The epidemiological effectiveness of dipyridamol, an interferon-inducing agent used for the prevention of influenza and viral acute respiratory diseases, was tested in 4 epidemiological trials, 3 of them carried out as double blind trials.
(9) These lanes encourage cyclists to 'ride in the gutter' which in itself is a very dangerous riding position – especially on busy congested roads as it places the cyclist right in a motorist's blind spot.
(10) A randomised double-blind trial comparing this preparation with a so-called 'shotgun' combination containing 0.05% betamethasone 17-valerate, 0.1% gentamicin, 1.0% tolnaftate and 1.0% clioquinol in 288 patients in the Philippines resulted in a better efficacy for the diflucortolone preparation in the 80 patients with bacterially or mycotically infected skin diseases.
(11) Blinded outcomes of depression and cognition were measured initially and twice in each phase.
(12) Therefore, two-dimensional echocardiographic findings in 22 patients with perivalvular abscess found at surgery or necropsy were compared with those in 24 patients without abscess in a retrospective but blinded study.
(13) In a double-blind trial, 50 patients with subcostal incisions performed for cholecystectomy or splenectomy, received 10 ml of either 0.5% bupivacaine plain or physiological saline twice daily by wound perfusion through an indwelling drainage tube for 3 days after operation.
(14) In a double-blind, randomized, within-patient comparative study, the efficacy and tolerability of Ro 14-9706 (an arotinoid methyl sulfone) in the treatment of actinic keratoses was compared with that of tretinoin (all-trans-retinoic acid).
(15) Eighteen adult epileptic patients under CBZ therapy were evaluated in this single-blind, randomized cross-over study.
(16) A total of 17 patients suffering from musculoskeletal disease were included in a double blind study to compare the efficacy and safety of piroxicam and indomethacin.
(17) Each subject applied a vehicle cream containing 0.075% capsaicin (Axsain, GalenPharma Inc.) to a 4 cm2 area of skin on one volar forearm and vehicle alone to an identical treatment area on the other forearm, according to a double-blind procedure.
(18) A prospective, double-blind study was undertaken to assess the effectiveness of oral dexamethasone premedication in reducing a variety of side effects associated with metrizamide myelography.
(19) They then entered, on a randomized and double-blind basis, a cross-over trial of two 16-week periods, blood pressure being measured fortnightly.
(20) The design was a single-blind randomised controlled study.