(1) We have examined the recombination potential of BLIN-1 by characterizing several independently derived kappa-expressing subclones for DNA rearrangement and V kappa gene usage.
(2) The BLIN-1 cell line is, to our knowledge, the first defined human model for examining this critical developmental stage in human B cell ontogeny.
(3) By polymerase chain reaction analyses, we detected transcripts from rearranged V-J-C kappa genes as well as transcripts from germline J-C kappa and V kappa in BLIN-1 cells induced to rearrange the kappa locus.
(4) Our collective results indicate that: (a) BLIN-1 can be induced to rearrange the kappa locus, and this correlates with the expression of germline kappa locus transcripts that may play a role in activating or targeting gene rearrangement; and (b) active rearrangement and usage of V genes representing different kappa families suggest that, like in the mouse, repertoire diversification in humans occurs in the presence of a fixed heavy chain rearrangement.
(5) Binding studies using radiolabeled IL-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a KD of 150pM.
(6) Analysis of Ig gene rearrangement and Ig expression in a series of BLIN-1 subclones show that the cells spontaneously rearrange kappa light chain genes, leading to the differentiation of surface kappa-negative pre-B cells into surface kappa-positive B cells.
(7) In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate.
(8) The human pre-B acute lymphoblastic leukemia cell line, BLIN-1, has been previously shown to undergo kappa light chain rearrangement in vitro, making it a valuable resource for analyzing pre-B to B cell differentiation.
(9) This cell line, designated BLIN-1, was initially established in tissue culture medium containing low m.w.
(10) BLIN-1 cells have a 9p chromosomal abnormality, identical to the abnormality present in the leukemic blasts from the patient's original bone marrow aspirate.
(11) The immunologic phenotype of BLIN-1 is consistent with a cell arrested at the pre-B cell stage of development.
Brin
Definition:
(n.) One of the radiating sticks of a fan. The outermost are larger and longer, and are called panaches.
Example Sentences:
(1) The idea that 80% of an engineer's time is spent on the day job and 20% pursuing a personal project is a mathematician's solution to innovation, Brin says.
(2) Brin and Page remain joint presidents, Brin in charge of technology, Page responsible for product launches, but the rapid growth of recent years has been steered by chief executive Eric Schmidt, 53, who came on board in 2001 as the commercial 'brain', negotiating the founders' evangelism and the shareholders' thirst for profits.
(3) The 11-year-old company, founded by Brin and Page in a garage in California, is the global search engine of choice, filtering what we find when we go looking on the internet.
(4) Brin's contention that censorship and "walled gardens", such as Apple's operating systems and Facebook's world of applications, will throttle the world of free and linked information on which Google has built its fortune may be right.
(5) As models for modern business managers, Brin and Page made their own rules.
(6) The latest prize from Milner, the Breakthrough Prize in Life Sciences , is a collaboration with his "old friends" Mark Zuckerberg of Facebook, and Sergey Brin of Google.
(7) Smartphones are "emasculating" – at least according to Sergey Brin , the co-founder of Google, who explained his view while addressing an audience wearing a computer headset that made him look slightly like a technological pirate.
(8) While Google did reach agreement with a variety of libraries, including those of Harvard and Oxford universities, like good Montessori students Page and Brin did not first ask the permission of publishers and authors before digitising their copyrighted books – backing off only after a lawsuit was filed.
(9) Ten years ago next month, in an innocuous suburban garage, Page and Brin, two geeky students at Stanford University, founded a company called Google.
(10) Brin comes from a family who fled antisemitism in the Soviet Union.
(11) Many consumers believe Google's search engine works on a formula that was created by founders Sergey Brin and Larry Page and that was that: they set it running and the rest is history.
(12) Brin and Page's mission is to 'organise the world's information and make it universally accessible and useful'.
(13) Google’s illustrious founders, Larry Page and Sergey Brin, sagely stated that “since it is very difficult even for experts to evaluate search engines, search engine bias is particularly insidious”.
(14) Brin, who is more sociable than Page, has his own quirks.
(15) The seeds for Google's success were planted by Page and Brin when they met as graduate students at Stanford in 1995.
(16) Brin said that he was moved to invest in the technology for animal welfare reasons.
(17) Co-founder Sergey Brin said that the company's social experiments had been more successful than it was given credit for – but that Buzz would be more than just talking with friends and playing games.
(18) The four traders – Daniel Brin, Scott Connelly, Karen Levine and Ryan Smith – also have 30 days to prove why they should not face civil penalties after the regulator said the actions had led to losses of around $140m for California and other US states.
(19) And appropriately for a company with such mighty ambitions, instead of one CEO decision-maker, Google has three: co-founders Larry Page and Sergey Brin plus their CEO, Eric Schmidt.
(20) Google is run by two youngish men, Larry Page and Sergey Brin , who are, in a literal sense, visionaries.