(1) We have examined the recombination potential of BLIN-1 by characterizing several independently derived kappa-expressing subclones for DNA rearrangement and V kappa gene usage.
(2) The BLIN-1 cell line is, to our knowledge, the first defined human model for examining this critical developmental stage in human B cell ontogeny.
(3) By polymerase chain reaction analyses, we detected transcripts from rearranged V-J-C kappa genes as well as transcripts from germline J-C kappa and V kappa in BLIN-1 cells induced to rearrange the kappa locus.
(4) Our collective results indicate that: (a) BLIN-1 can be induced to rearrange the kappa locus, and this correlates with the expression of germline kappa locus transcripts that may play a role in activating or targeting gene rearrangement; and (b) active rearrangement and usage of V genes representing different kappa families suggest that, like in the mouse, repertoire diversification in humans occurs in the presence of a fixed heavy chain rearrangement.
(5) Binding studies using radiolabeled IL-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a KD of 150pM.
(6) Analysis of Ig gene rearrangement and Ig expression in a series of BLIN-1 subclones show that the cells spontaneously rearrange kappa light chain genes, leading to the differentiation of surface kappa-negative pre-B cells into surface kappa-positive B cells.
(7) In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate.
(8) The human pre-B acute lymphoblastic leukemia cell line, BLIN-1, has been previously shown to undergo kappa light chain rearrangement in vitro, making it a valuable resource for analyzing pre-B to B cell differentiation.
(9) This cell line, designated BLIN-1, was initially established in tissue culture medium containing low m.w.
(10) BLIN-1 cells have a 9p chromosomal abnormality, identical to the abnormality present in the leukemic blasts from the patient's original bone marrow aspirate.
(11) The immunologic phenotype of BLIN-1 is consistent with a cell arrested at the pre-B cell stage of development.
Cease
Definition:
(v. i.) To come to an end; to stop; to leave off or give over; to desist; as, the noise ceased.
(v. i.) To be wanting; to fail; to pass away.
(v. t.) To put a stop to; to bring to an end.
(n.) Extinction.
Example Sentences:
(1) Calcium added to the myocytes seen after beating ceased reversed the effect and the cells started to beat again.
(2) This study demonstrates that 1) complete AV block is not a contraindication to the Fontan operation, 2) some patients may not require AV synchrony postoperatively for survival, and 3) postoperative atrial flutter or fibrillation may cease or be easier to control after the Fontan operation.
(3) Sodium taurolithocholate, a monohydroxy bile salt, does not affect the CD spectrum of CEase, and neither the di- or the monohydroxy bile salt activates the enzyme.
(4) At our current rate, which is 10 gigatons of carbon a year, we have 27 years left, after which time carbon emissions would need to cease.
(5) "The level of the financial penalty to be imposed in this case should be sufficient to act as an effective incentive [to all broadcast licence holders] to continue to provide all elements of their respective licensed services throughout the licensed period, even if the licensee believes that there are commercial reasons for it to cease providing all or part of the licensed service during the licence period," the regulator added.
(6) As long as Israel refuses to cease settlement activities and to the release of the fourth group of Palestinian prisoners in accordance with our agreements, they leave us no choice but to insist that we will not remain the only ones committed to the implementation of these agreements, while Israel continuously violates them,” Abbas said.
(7) On the other hand, DNA synthesis of the acrD mutant cells ceased soon after the shift-up, and the cell mass did not appreciably increase during the prolonged incubation.
(8) Paracoccus (Micrococcus) denitrificans and Escherichia coli oxidizing succinate rapidly ceased to reduce nitrate when oxygen was available, and equally rapidly commenced nitrate reduction when all the oxygen had been consumed.
(9) The channels usually ceased conducting within a few minutes after seal formation with the patch pipette and could not be re-activated with depolarizing voltage steps.
(10) This reaction gave rise to artifacts in alkaline polyacrylamide gels and isoelectric focusing systems when residual acrylamide monomers were still present in the gel matrix after the polymerization process ceased.
(11) Sepah’s officers told him he must quit writing and cease his promotion of Kurdish autonomy or it would be years before he knew freedom again.
(12) Urinary frequency was normalized in 6 out of 16 (37.5%), urgency ceased in 6 out of 17 (35.7%) and urgent incontinence disappeared in 9 out of 14 (50%) patients.
(13) The presence of urinary-bladder-stones was verified cystoscopically and the clinical symptomatology ceased promptly after removal of the concrements.
(14) The time-related incidence of these cells entities--the appearance of "dusk" and "bright" cells at 5 min, transitory domination of "bright" cells and the nadir of "dusk" cells at 20 min, sporadic recognition of "bright" cells, lack of "dusk" cells at 45 min and the absence of both cell forms at 180 min--displayed that LP-reactive response promptly appeared and rapidly ceased.
(15) Vomiting ceased in 85% of the symptomatic patients; pulmonary deterioration was halted, and the frequency of aspiration pneumonia was reduced in 68%; nutritional improvement was seen in 44%; the hydration status improved in 88%; and the frequency of hospital admissions decreased in 74%.
(16) The peak closure period was between January and June 2009 when 52 pubs ceased trading every week, and there are now 54,490 pubs left in the country.
(17) Of the pathogenetic mechanisms involved, increasing importance is now attached to immunological responses and intravascular coagulopathies, though other processes long known to medicine have not ceased to play their part.
(18) He did not improve with anticoagulation, but the episodes ceased promptly after the administration of an anticonvulsant.
(19) Inhibition of protein synthesis in log cultures by the addition of chloramphenicol or amino acid starvation allows ColE1 DNA to continue replicating long after chromosomal replication has ceased.
(20) Intraventricular injection of atropine during the development of fever caused an inhibition of shievering and a decrease in O2 consumption so that temperature ceased to rise and returned to normal.