(1) We have examined the recombination potential of BLIN-1 by characterizing several independently derived kappa-expressing subclones for DNA rearrangement and V kappa gene usage.
(2) The BLIN-1 cell line is, to our knowledge, the first defined human model for examining this critical developmental stage in human B cell ontogeny.
(3) By polymerase chain reaction analyses, we detected transcripts from rearranged V-J-C kappa genes as well as transcripts from germline J-C kappa and V kappa in BLIN-1 cells induced to rearrange the kappa locus.
(4) Our collective results indicate that: (a) BLIN-1 can be induced to rearrange the kappa locus, and this correlates with the expression of germline kappa locus transcripts that may play a role in activating or targeting gene rearrangement; and (b) active rearrangement and usage of V genes representing different kappa families suggest that, like in the mouse, repertoire diversification in humans occurs in the presence of a fixed heavy chain rearrangement.
(5) Binding studies using radiolabeled IL-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a KD of 150pM.
(6) Analysis of Ig gene rearrangement and Ig expression in a series of BLIN-1 subclones show that the cells spontaneously rearrange kappa light chain genes, leading to the differentiation of surface kappa-negative pre-B cells into surface kappa-positive B cells.
(7) In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate.
(8) The human pre-B acute lymphoblastic leukemia cell line, BLIN-1, has been previously shown to undergo kappa light chain rearrangement in vitro, making it a valuable resource for analyzing pre-B to B cell differentiation.
(9) This cell line, designated BLIN-1, was initially established in tissue culture medium containing low m.w.
(10) BLIN-1 cells have a 9p chromosomal abnormality, identical to the abnormality present in the leukemic blasts from the patient's original bone marrow aspirate.
(11) The immunologic phenotype of BLIN-1 is consistent with a cell arrested at the pre-B cell stage of development.
Desist
Definition:
(v. i.) To cease to proceed or act; to stop; to forbear; -- often with from.
Example Sentences:
(1) The resolution also opens the door for other bodies, such as the European Union and the International Criminal Court, to intensify their pressure on Israel to desist from its illegal practices on the West Bank and its war crimes in Gaza.
(2) We suspect that this hazard is more prevalent than its scarcity in the literature would suggest and that potential for unintended injury should be a prominent factor in the decision to proceed or desist with resection of a large neuroblastoma.
(3) Manchester United manager Ed Woodward is reported to have sent Chelsea a "terse" letter, warning them to cease and desist in their efforts to sign Wayne Rooney .
(4) Functions for the probability of feeding success and desistance over time were derived using data from observations on 300 mosquitoes.
(5) Last week the service pleaded with the public to desist from killing wild animals and instead contact the nearest office of the service.
(6) The agency’s ability to mute the proceedings was a surprise to Pohl, who issued a cease-and-desist order .
(7) He also told those briefing against Ed Miliband to desist, saying they should "get over it" and realise they had lost the Labour leadership election.
(8) Similar 22-kilohertz vocalizations occur in other social contexts, and in general they appear to be desist-contact signals.
(9) UN secretary-general Ban Ki-moon's office has said he is "gravely concerned" and has called on all sides to exercise the utmost restraint and desist from provocative actions: "He strongly condemns the excessive use of force by government security forces against unarmed protestors in the capital Sana'a, resulting in scores of people killed and many more injured."
(10) Britain has been an enthusiastic advocate of EU and US sanctions imposed on Russia over the Ukraine conflict, and on Wednesday Cameron warned Putin that if he did not desist from supporting the rebels there would have financial and economic consequences for his country for many years to come.
(11) The authors desist from proposing genus and species names for the same reasons.
(12) Does a desisting doctor share responsibility, if he refers a patient to another doctor, who he knows would willingly perform prenatal diagnostics?
(13) Kaká came on to help Madrid seek it but Jupp Heynckes's side did not desist.
(14) The author points out the need for desisting from a scientific posture that switches the professional practice of Psychology into a mere diffusing of the Philosophy of Behavior--instead of resorting to technics aiming at modifying a behavior the patient feels as unpleasant or unadapted.
(15) So we have to be very firm and strong about the sanctions and say to Vladimir Putin: ‘What you are doing is unacceptable and it will have economic and financial consequences for many years to come if you do not desist with your behaviour’.” Speaking during a visit to West Sussex, Cameron underlined his intention to keep pressure on European Union partners to maintain the sanctions regime against Russia despite the ceasefire agreement.
(16) Houston’s city attorney, David Feldman, sent an email to Uber last month formally asking that it “cease and desist” from encouraging the public to write to officials demanding the introduction of the service.
(17) Two years later, in the summer of 2010, UberCab opened in San Francisco with just a small fleet of cars and a handful of employees, to be greeted by a cease-and-desist order from the city’s municipal transportation agency.
(18) Michael Gove may decry criticism of British leadership as an “out-of-touch elite”, but aerial photographs have proved that while the German defence had constructed concrete bunkers four deep, as late as 1916 old Oxford cavalrymen like Haig – drawn from class not qualification – desisted from resourcing trench warfare, insisting that a breakthrough was still possible.
(19) "It's a very serious situation - the message from the United States is Iran should cease and desist."
(20) Most of the time when we get issues like that coming to us we send out a desist notice and we say to the press, 'Look, there's an issue here, you may be in breach of the code, you got those photographs by harassment, you've got an issue to do with the privacy of that family, hold back' and they do.