(1) We have examined the recombination potential of BLIN-1 by characterizing several independently derived kappa-expressing subclones for DNA rearrangement and V kappa gene usage.
(2) The BLIN-1 cell line is, to our knowledge, the first defined human model for examining this critical developmental stage in human B cell ontogeny.
(3) By polymerase chain reaction analyses, we detected transcripts from rearranged V-J-C kappa genes as well as transcripts from germline J-C kappa and V kappa in BLIN-1 cells induced to rearrange the kappa locus.
(4) Our collective results indicate that: (a) BLIN-1 can be induced to rearrange the kappa locus, and this correlates with the expression of germline kappa locus transcripts that may play a role in activating or targeting gene rearrangement; and (b) active rearrangement and usage of V genes representing different kappa families suggest that, like in the mouse, repertoire diversification in humans occurs in the presence of a fixed heavy chain rearrangement.
(5) Binding studies using radiolabeled IL-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a KD of 150pM.
(6) Analysis of Ig gene rearrangement and Ig expression in a series of BLIN-1 subclones show that the cells spontaneously rearrange kappa light chain genes, leading to the differentiation of surface kappa-negative pre-B cells into surface kappa-positive B cells.
(7) In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate.
(8) The human pre-B acute lymphoblastic leukemia cell line, BLIN-1, has been previously shown to undergo kappa light chain rearrangement in vitro, making it a valuable resource for analyzing pre-B to B cell differentiation.
(9) This cell line, designated BLIN-1, was initially established in tissue culture medium containing low m.w.
(10) BLIN-1 cells have a 9p chromosomal abnormality, identical to the abnormality present in the leukemic blasts from the patient's original bone marrow aspirate.
(11) The immunologic phenotype of BLIN-1 is consistent with a cell arrested at the pre-B cell stage of development.
Stay
Definition:
(n.) A large, strong rope, employed to support a mast, by being extended from the head of one mast down to some other, or to some part of the vessel. Those which lead forward are called fore-and-aft stays; those which lead to the vessel's side are called backstays. See Illust. of Ship.
(v. i.) To stop from motion or falling; to prop; to fix firmly; to hold up; to support.
(v. i.) To support from sinking; to sustain with strength; to satisfy in part or for the time.
(v. i.) To bear up under; to endure; to support; to resist successfully.
(v. i.) To hold from proceeding; to withhold; to restrain; to stop; to hold.
(v. i.) To hinde/; to delay; to detain; to keep back.
(v. i.) To remain for the purpose of; to wait for.
(v. i.) To cause to cease; to put an end to.
(v. i.) To fasten or secure with stays; as, to stay a flat sheet in a steam boiler.
(v. i.) To tack, as a vessel, so that the other side of the vessel shall be presented to the wind.
(v. i.) To remain; to continue in a place; to abide fixed for a space of time; to stop; to stand still.
(v. i.) To continue in a state.
(v. i.) To wait; to attend; to forbear to act.
(v. i.) To dwell; to tarry; to linger.
(v. i.) To rest; to depend; to rely; to stand; to insist.
(v. i.) To come to an end; to cease; as, that day the storm stayed.
(v. i.) To hold out in a race or other contest; as, a horse stays well.
(v. i.) To change tack; as a ship.
(n.) That which serves as a prop; a support.
(n.) A corset stiffened with whalebone or other material, worn by women, and rarely by men.
(n.) Continuance in a place; abode for a space of time; sojourn; as, you make a short stay in this city.
(n.) Cessation of motion or progression; stand; stop.
(n.) Hindrance; let; check.
(n.) Restraint of passion; moderation; caution; steadiness; sobriety.
(n.) Strictly, a part in tension to hold the parts together, or stiffen them.
Example Sentences:
(1) Overall length of stay found in this study (14.02 days) is considerably higher than Indian optimum.
(2) The standard varies from modest to lavish – choose carefully and you could be staying in an antique-filled room with your host's paintings on the walls, and breakfasting on the veranda of a tropical garden.
(3) A total of 1,268 patients admitted to hospital wards were kept under surveillance by one observer throughout their stay in hospital.
(4) We are better off in.” Out campaigners have claimed that the NHS could be badly hit by a decision to stay in the EU.
(5) Eighty-five per cent of newly appointed judges in France are women because the men stay away.
(6) In this way, we tried to find out how the patients experience the treatment and stay on the Unit, what is most helpful in solving their problems and what are, in their opinion, the direct gains of hospitalization.
(7) "If older people do not stay informed about the changes and take action, there is a danger that they will end up paying more unnecessarily."
(8) In Phase 2 (two minutes after injection) all parameters return to their control values ; except CVP which stays elevated.
(9) Enright said: “We call on the home secretary and chair of IICSA [the independent inquiry into child sexual abuse] to engage actively and urgently to find a way forward that secures the confidence of survivors and provides the inquiry’s legal team with the resources and support they need to deliver justice and truth that survivors deserve.” Stein said his clients were “deeply disatisfied” with aspects of how the inquiry had been conducted but called for Emmerson to stay, adding: “I urge the home secretary to seek to find a way in which his valuable contribution can be maintained”.
(10) Read more After Monday’s launch at 7.30am (11.30pm GMT), the taikonauts will dock with the Tiangong 2 space laboratory, where they will spend about a month, testing systems and processes for space stays and refuelling, and doing scientific experiments.
(11) Silvio Berlusconi's government is battling to stay in the eurozone against mounting odds – not least the country's mountain of state debt, which is the largest in the single currency area.
(12) Approximately 16,000 people were diagnosed with multidrug-resistant tuberculosis in 2012 but were not given the treatment they needed to stay alive and prevent the spread of the disease, the World Health Organisation (WHO) has said.
(13) While ITV1's Harry Hill and the final series of BBC1's Gavin and Stacey will stay put, Sky1 did manage to secure US drama House, starring Hugh Laurie, from Channel Five, paying an estimated £500,000 an episode.
(14) After filming, he stayed on in the Middle East for several weeks to travel.
(15) Patients identified sources of stress associated with their ICU stay, yet most (76%) rated their ICU experience positively.
(16) To be faced with not being able to stay with or even be near their baby is inconceivable."
(17) Long-stay psychiatric in-patients in South Glamorgan were reviewed using the MRC Needs for Care assessment.
(18) Make Quinn stay with B613 I think it would be difficult to bring her back to the fold at Pope and Associates (unless they’re playing the long con and her infiltration of B613 is part of the plan), but her anger would be well utilized against her former coworkers.
(19) The majority of them were able to perceive a connection between their worsened skin condition and the acute psychosocial constellation during their brief stay at home.
(20) They’re staying home,” Cruz declared in his speech.