(1) We have examined the recombination potential of BLIN-1 by characterizing several independently derived kappa-expressing subclones for DNA rearrangement and V kappa gene usage.
(2) The BLIN-1 cell line is, to our knowledge, the first defined human model for examining this critical developmental stage in human B cell ontogeny.
(3) By polymerase chain reaction analyses, we detected transcripts from rearranged V-J-C kappa genes as well as transcripts from germline J-C kappa and V kappa in BLIN-1 cells induced to rearrange the kappa locus.
(4) Our collective results indicate that: (a) BLIN-1 can be induced to rearrange the kappa locus, and this correlates with the expression of germline kappa locus transcripts that may play a role in activating or targeting gene rearrangement; and (b) active rearrangement and usage of V genes representing different kappa families suggest that, like in the mouse, repertoire diversification in humans occurs in the presence of a fixed heavy chain rearrangement.
(5) Binding studies using radiolabeled IL-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a KD of 150pM.
(6) Analysis of Ig gene rearrangement and Ig expression in a series of BLIN-1 subclones show that the cells spontaneously rearrange kappa light chain genes, leading to the differentiation of surface kappa-negative pre-B cells into surface kappa-positive B cells.
(7) In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate.
(8) The human pre-B acute lymphoblastic leukemia cell line, BLIN-1, has been previously shown to undergo kappa light chain rearrangement in vitro, making it a valuable resource for analyzing pre-B to B cell differentiation.
(9) This cell line, designated BLIN-1, was initially established in tissue culture medium containing low m.w.
(10) BLIN-1 cells have a 9p chromosomal abnormality, identical to the abnormality present in the leukemic blasts from the patient's original bone marrow aspirate.
(11) The immunologic phenotype of BLIN-1 is consistent with a cell arrested at the pre-B cell stage of development.
Stop
Definition:
(v. t.) To close, as an aperture, by filling or by obstructing; as, to stop the ears; hence, to stanch, as a wound.
(v. t.) To obstruct; to render impassable; as, to stop a way, road, or passage.
(v. t.) To arrest the progress of; to hinder; to impede; to shut in; as, to stop a traveler; to stop the course of a stream, or a flow of blood.
(v. t.) To hinder from acting or moving; to prevent the effect or efficiency of; to cause to cease; to repress; to restrain; to suppress; to interrupt; to suspend; as, to stop the execution of a decree, the progress of vice, the approaches of old age or infirmity.
(v. t.) To regulate the sounds of, as musical strings, by pressing them against the finger board with the finger, or by shortening in any way the vibrating part.
(v. t.) To point, as a composition; to punctuate.
(v. t.) To make fast; to stopper.
(v. i.) To cease to go on; to halt, or stand still; to come to a stop.
(v. i.) To cease from any motion, or course of action.
(v. i.) To spend a short time; to reside temporarily; to stay; to tarry; as, to stop with a friend.
(n.) The act of stopping, or the state of being stopped; hindrance of progress or of action; cessation; repression; interruption; check; obstruction.
(n.) That which stops, impedes, or obstructs; as obstacle; an impediment; an obstruction.
(n.) A device, or piece, as a pin, block, pawl, etc., for arresting or limiting motion, or for determining the position to which another part shall be brought.
(n.) The closing of an aperture in the air passage, or pressure of the finger upon the string, of an instrument of music, so as to modify the tone; hence, any contrivance by which the sounds of a musical instrument are regulated.
(n.) In the organ, one of the knobs or handles at each side of the organist, by which he can draw on or shut off any register or row of pipes; the register itself; as, the vox humana stop.
(n.) A member, plain or molded, formed of a separate piece and fixed to a jamb, against which a door or window shuts. This takes the place, or answers the purpose, of a rebate. Also, a pin or block to prevent a drawer from sliding too far.
(n.) A point or mark in writing or printing intended to distinguish the sentences, parts of a sentence, or clauses; a mark of punctuation. See Punctuation.
(n.) The diaphragm used in optical instruments to cut off the marginal portions of a beam of light passing through lenses.
(n.) The depression in the face of a dog between the skull and the nasal bones. It is conspicuous in the bulldog, pug, and some other breeds.
(n.) Some part of the articulating organs, as the lips, or the tongue and palate, closed (a) so as to cut off the passage of breath or voice through the mouth and the nose (distinguished as a lip-stop, or a front-stop, etc., as in p, t, d, etc.), or (b) so as to obstruct, but not entirely cut off, the passage, as in l, n, etc.; also, any of the consonants so formed.
Example Sentences:
(1) Decreased MU stops additions of bone by modeling and increases removal of bone next to marrow by remodeling.
(2) The stopped-flow technique was used to measure the rate constants for the reactions between the oxidized forms of peroxidase with luminol and the following substrates: p-iodophenol, p-bromophenol, p-clorophenol, o-iodophenol, m-iodophenol, luciferin, and 2-iodo-6-hydroxybenzothiazole.
(3) The region containing the injection stop signal (iss) has been cloned and sequenced and found to contain numerous large repeats and inverted repeats which may be part of the iss.
(4) Certainly, Saunders did not land a single blow that threatened to stop his opponent, although he took quite a few himself that threatened his titles in the final few rounds.
(5) … or a theatre and concert hall There are a total of 16 ghost stations on the Paris metro; stops that were closed or never opened.
(6) All of this in the same tones of weary nonchalance you might use to stop the dog nosing around in the bin.
(7) There are no oceans wide enough to stop us from dreaming.
(8) At the ceremony, the Taliban welcomed dialogue with Washington but said their fighters would not stop fighting.
(9) In a separate exclusive interview , Alexis Tsipras, the increasingly powerful 37-year-old Greek politician now regarded by many as holding the future of the euro in his hands, told the Guardian that he was determined "to stop the experiment" with austerity policies imposed by Germany.
(10) She stopped working only when the pain made it hard for her to get to work.
(11) A tall young Border Police officer stopped me, his rifle cradled in his arms.
(12) Crown prince Sultan Bin Abdel Aziz said yesterday that the state had "spared no effort" to avoid such disasters but added that "it cannot stop what God has preordained.
(13) Control measures were introduced rapidly, effectively stopping the epidemic.
(14) Both strong-stop DNAs are made early during in vitro reactions and decline in concentration later, consistent with postulated roles as initiators of long minus- and plus-strand DNA.
(15) Thus it appears that a portion of the adaptation to prolonged and intense endurance training that is responsible for the higher lactate threshold in the trained state persists for a long time (greater than 85 days) after training is stopped.
(16) When asked why the streets of London were not heaving with demonstrators protesting against Russia turning Aleppo into the Guernica of our times, Stop the War replied that it had no wish to add to the “jingoism” politicians were whipping up against plucky little Russia .
(17) Bacteria can stop or lessen antibodies synthesis process.
(18) Never become so enamored of your own smarts that you stop signing up for life’s hard classes.
(19) The scatter measurement was made using a standard imaging geometry with both beam stops and an additional x-ray detector placed behind the standard imaging detector.
(20) Thirteen of the dogs treated with various drug regimens lived for 90 days, after which time treatment was stopped; 10 of the dogs eventually rejected the grafts, but three had continued graft function for 6 months or longer and may be permanently tolerant.