What's the difference between blot and spot?

Blot


Definition:

  • (v. t.) To spot, stain, or bespatter, as with ink.
  • (v. t.) To impair; to damage; to mar; to soil.
  • (v. t.) To stain with infamy; to disgrace.
  • (v. t.) To obliterate, as writing with ink; to cancel; to efface; -- generally with out; as, to blot out a word or a sentence. Often figuratively; as, to blot out offenses.
  • (v. t.) To obscure; to eclipse; to shadow.
  • (v. t.) To dry, as writing, with blotting paper.
  • (v. i.) To take a blot; as, this paper blots easily.
  • (n.) A spot or stain, as of ink on paper; a blur.
  • (n.) An obliteration of something written or printed; an erasure.
  • (n.) A spot on reputation; a stain; a disgrace; a reproach; a blemish.
  • (n.) An exposure of a single man to be taken up.
  • (n.) A single man left on a point, exposed to be taken up.
  • (n.) A weak point; a failing; an exposed point or mark.

Example Sentences:

  • (1) Western blot analysis of these mitochondria using an antibody against carnitine palmitoyltransferase II purified from beef heart demonstrates a 68-kDa protein, which under ischemic conditions apparently is decreased by 2 kDa.
  • (2) We have developed a new procedure for the rapid preparation of undegraded total RNA from cultured cells for specific quantitation by dot blotting analysis.
  • (3) Only the approximately 2.7 kb mRNA species was visualized in Northern blots of total cellular and poly(A+) RNA isolated from cardiac ventricular muscle.
  • (4) In addition to performing Northern and Western blots for tyrosinase, tyrosine hydroxylase activity (THA) and dopa oxidase activity (DOA) were measured.
  • (5) After Western blot, 2 of the 5 protein bands of swine-cag (27 and 57 kD) and 3 of the 8 protein bands of human cag (27, 32, and 57 kD) reacted with the anti-Toxoplasma antibody used in the ELISA.
  • (6) Northern blot analysis with an 18-mer radiolabelled oligonucleotide, derived from an ALP specific cDNA clone, revealed a specific mRNA of about 700-800 nucleotides in HS-24 tumor cells.
  • (7) We have examined the pattern of bFGF expression during CNS development using protein immunoblot and RNA blot analyses.
  • (8) Therefore, we conclude this is a bovine DR beta-like pseudogene, BoDR beta I. Exon-containing regions have been used as probes in Southern blot analyses of bovine genomic DNA digested with EcoRI.
  • (9) Three distinct antigenic regions of bovine somatotropin (bST) were identified on the basis of the ability of a set of monoclonal antibodies to bind to proteolytic fragments and deletion variants of recombinant bST (rbST) in Western blot analyses.
  • (10) cDNA isolations, RNA, and genomic DNA blots confirm the existence and expression of two genes that produce indistinguishable SOD-4 proteins.
  • (11) We studied the feasibility of using RNA and DNA from autopsies for Northern and Southern blot analysis.
  • (12) Analysis of glutamate dehydrogenase (GDH) expression by enzyme assay and immunoblots, as well as Northern and dot blots of poly (A)+ RNA, in the deletion strains indicates that there are two upstream regulatory sequences that control the level of gene expression.
  • (13) The probe encoding LHCPII hybridizes to RNAs of 9.5 and 6.6 kb on northern blots of total RNA while the 3'-end probe hybridizes only to the 6.6 kb RNA.
  • (14) Enzyme activities were measured on nitrocellulose blots by using pure enzyme preparations as well as Triton X-100-solubilized membranes.
  • (15) The results indicated a very good comparability between the dot-blot assay and IF-tests, and this dot-blot method was ascertained as a simple and useful method for the scrub typhus serodiagnosis.
  • (16) An increase in cytoplasmic PRL mRNA content was evident in all the animals treated with estrogen as revealed with cytoplasmic dot blot analysis.
  • (17) All these strains produced an enterotoxic principle, antigenically related to cholera coli family of enterotoxins, as detected by latex agglutination and immuno-dot-blot tests.
  • (18) These alterations were not dependent on the prophage integration prior to curing, and no phage DNA was detected in cured cells by blot hybridisation.
  • (19) This study shows that the sensitivity and specificity of in situ hybridisation for the detection of EBV genomes in AIDS related lymphomas approaches that of Southern blotting, even when using routinely processed archival, paraffin wax embedded material.
  • (20) Western blot analysis revealed that the effect of PMA on U-373MG cells shows specificity in that GFA protein levels decline, while those of other major cytoskeletal proteins were unaltered.

Spot


Definition:

  • (n.) A mark on a substance or body made by foreign matter; a blot; a place discolored.
  • (n.) A stain on character or reputation; something that soils purity; disgrace; reproach; fault; blemish.
  • (n.) A small part of a different color from the main part, or from the ground upon which it is; as, the spots of a leopard; the spots on a playing card.
  • (n.) A small extent of space; a place; any particular place.
  • (n.) A variety of the common domestic pigeon, so called from a spot on its head just above its beak.
  • (n.) A sciaenoid food fish (Liostomus xanthurus) of the Atlantic coast of the United States. It has a black spot behind the shoulders and fifteen oblique dark bars on the sides. Called also goody, Lafayette, masooka, and old wife.
  • (n.) The southern redfish, or red horse, which has a spot on each side at the base of the tail. See Redfish.
  • (n.) Commodities, as merchandise and cotton, sold for immediate delivery.
  • (v. t.) To make visible marks upon with some foreign matter; to discolor in or with spots; to stain; to cover with spots or figures; as, to spot a garnment; to spot paper.
  • (v. t.) To mark or note so as to insure recognition; to recognize; to detect; as, to spot a criminal.
  • (v. t.) To stain; to blemish; to taint; to disgrace; to tarnish, as reputation; to asperse.
  • (v. i.) To become stained with spots.

Example Sentences:

  • (1) O'Connell first spotted 14-year-old David Rudisha in 2004, running the 200m sprint at a provincial schools race.
  • (2) These lanes encourage cyclists to 'ride in the gutter' which in itself is a very dangerous riding position – especially on busy congested roads as it places the cyclist right in a motorist's blind spot.
  • (3) The effect of the mutation for white belly spot controlled by the dominant gene W on spermatogenesis in mice was examined by experimental cryptorchidism and its surgical reversal.
  • (4) Moments later, explosive charges blasted free two tungsten blocks, to shift the balance of the probe so it could fly itself to a prearranged landing spot .
  • (5) Our findings: (1) both forms, LC1 and LC3, migrate in the two species with rather similar electrophoretic constants (both in terms of pI and Mr); (2) the LC2 forms of rabbit and humans exhibit the same Mr but quite different pI values, the rabbit forms being more acidic; (3) the chain LC2Sb is resolved into two spots in both rabbit and humans.
  • (6) You just have to be the first person to spot a coach.
  • (7) The overall effect achieved with LTS was less than that with LTP, but it is possible to reduce IOP by selecting more reasonable parameters, e.g., a laser setting of 200mW, a 50 microns spot size and a 0.5 to 1-sec duration.
  • (8) The average repetitive yields and initial coupling of proteins spotted or blotted into PVDF membranes ranged between 84-98% and 30-108% respectively, and were comparable with the yields measured for proteins spotted onto Polybrene-coated glass fiber discs.
  • (9) In north-west Copenhagen, among the quiet, graffiti-tagged streets of red-brick blocks and low-rise social housing bordering the multi-ethnic Nørrebro district, police continued to cordon off roads and search a flat near the spot where officers killed a man believed to be behind Denmark’s bloodiest attacks in over a decade.
  • (10) Detection limits were then calculated for the different sizes of cold spots.
  • (11) These complications were believed to be caused by the use of a small spot size with high energy.
  • (12) In the brownish skin and in the black spots of the dorsal region all types of chromatophores are found.
  • (13) I can always spot something for my sisters Gretchen and Amy.
  • (14) Debeaking had no effect on the incidence of blood-spotting in the eggs.
  • (15) Six abnormal colonoscopic appearances were documented, namely mucosal edema, ulcers, friability, punctate spots, erythematous areas and luminal exudate.
  • (16) The town's Castle Hill is the perfect climb for travellers with energy to burn off: at the top is a picnic spot with far-reaching views, and there is a small children's play area at its foot.
  • (17) This appears to be a newly described entity, although it resembles a Becker's nevus without hypertrichosis or an typical café au lait spot.
  • (18) A £100,000 bronze statue of an ordinary family, the Joneses, will be unveiled in a prime spot outside the city’s library which opened last year.
  • (19) When delta phi was enlarged, first saccades were either directed near the green or the red spot (bistable response mode).
  • (20) Join us for a spot of future gazing as we discuss: The challenges and opportunities colleges and training providers will face over the next five years International expansion The role of FE in higher education New ways to diversify New technology – the possibilities and risks.