What's the difference between blot and technique?

Blot


Definition:

  • (v. t.) To spot, stain, or bespatter, as with ink.
  • (v. t.) To impair; to damage; to mar; to soil.
  • (v. t.) To stain with infamy; to disgrace.
  • (v. t.) To obliterate, as writing with ink; to cancel; to efface; -- generally with out; as, to blot out a word or a sentence. Often figuratively; as, to blot out offenses.
  • (v. t.) To obscure; to eclipse; to shadow.
  • (v. t.) To dry, as writing, with blotting paper.
  • (v. i.) To take a blot; as, this paper blots easily.
  • (n.) A spot or stain, as of ink on paper; a blur.
  • (n.) An obliteration of something written or printed; an erasure.
  • (n.) A spot on reputation; a stain; a disgrace; a reproach; a blemish.
  • (n.) An exposure of a single man to be taken up.
  • (n.) A single man left on a point, exposed to be taken up.
  • (n.) A weak point; a failing; an exposed point or mark.

Example Sentences:

  • (1) Western blot analysis of these mitochondria using an antibody against carnitine palmitoyltransferase II purified from beef heart demonstrates a 68-kDa protein, which under ischemic conditions apparently is decreased by 2 kDa.
  • (2) We have developed a new procedure for the rapid preparation of undegraded total RNA from cultured cells for specific quantitation by dot blotting analysis.
  • (3) Only the approximately 2.7 kb mRNA species was visualized in Northern blots of total cellular and poly(A+) RNA isolated from cardiac ventricular muscle.
  • (4) In addition to performing Northern and Western blots for tyrosinase, tyrosine hydroxylase activity (THA) and dopa oxidase activity (DOA) were measured.
  • (5) After Western blot, 2 of the 5 protein bands of swine-cag (27 and 57 kD) and 3 of the 8 protein bands of human cag (27, 32, and 57 kD) reacted with the anti-Toxoplasma antibody used in the ELISA.
  • (6) Northern blot analysis with an 18-mer radiolabelled oligonucleotide, derived from an ALP specific cDNA clone, revealed a specific mRNA of about 700-800 nucleotides in HS-24 tumor cells.
  • (7) We have examined the pattern of bFGF expression during CNS development using protein immunoblot and RNA blot analyses.
  • (8) Therefore, we conclude this is a bovine DR beta-like pseudogene, BoDR beta I. Exon-containing regions have been used as probes in Southern blot analyses of bovine genomic DNA digested with EcoRI.
  • (9) Three distinct antigenic regions of bovine somatotropin (bST) were identified on the basis of the ability of a set of monoclonal antibodies to bind to proteolytic fragments and deletion variants of recombinant bST (rbST) in Western blot analyses.
  • (10) cDNA isolations, RNA, and genomic DNA blots confirm the existence and expression of two genes that produce indistinguishable SOD-4 proteins.
  • (11) We studied the feasibility of using RNA and DNA from autopsies for Northern and Southern blot analysis.
  • (12) Analysis of glutamate dehydrogenase (GDH) expression by enzyme assay and immunoblots, as well as Northern and dot blots of poly (A)+ RNA, in the deletion strains indicates that there are two upstream regulatory sequences that control the level of gene expression.
  • (13) The probe encoding LHCPII hybridizes to RNAs of 9.5 and 6.6 kb on northern blots of total RNA while the 3'-end probe hybridizes only to the 6.6 kb RNA.
  • (14) Enzyme activities were measured on nitrocellulose blots by using pure enzyme preparations as well as Triton X-100-solubilized membranes.
  • (15) The results indicated a very good comparability between the dot-blot assay and IF-tests, and this dot-blot method was ascertained as a simple and useful method for the scrub typhus serodiagnosis.
  • (16) An increase in cytoplasmic PRL mRNA content was evident in all the animals treated with estrogen as revealed with cytoplasmic dot blot analysis.
  • (17) All these strains produced an enterotoxic principle, antigenically related to cholera coli family of enterotoxins, as detected by latex agglutination and immuno-dot-blot tests.
  • (18) These alterations were not dependent on the prophage integration prior to curing, and no phage DNA was detected in cured cells by blot hybridisation.
  • (19) This study shows that the sensitivity and specificity of in situ hybridisation for the detection of EBV genomes in AIDS related lymphomas approaches that of Southern blotting, even when using routinely processed archival, paraffin wax embedded material.
  • (20) Western blot analysis revealed that the effect of PMA on U-373MG cells shows specificity in that GFA protein levels decline, while those of other major cytoskeletal proteins were unaltered.

Technique


Definition:

  • (n.) Same as Technic, n.

Example Sentences:

  • (1) All transplants were performed using standard techniques, the operation for the two groups differing only as described above.
  • (2) The technique is facilitated by an amazingly low tendency to bleeding.
  • (3) Renal micropuncture and microdissection techniques with ultramicro fluid analysis have been applied to evaluate single nephron function in the skate, Raja erinacea.
  • (4) Use of the improved operative technique contributed to reduction in number of complications.
  • (5) We conclude that first-transit and blood-pool techniques are equally accurate methods for determining EF when the time-activity method of analysis is employed.
  • (6) Multiple overlapping thin 3D slab acquisition is presented as a magnitude contrast (time of flight) technique which combines advantages from multiple thin slice 2D and direct 3D volume acquisitions to obtain high-resolution cross-sectional images of vessel detail.
  • (7) ASF-II was purified to apparent homogeneity by using concanavalin A-agarose affinity chromatography, DEAE-cellulose chromatography, alumina gel adsorption, and isoelectric focussing techniques.
  • (8) When the data correlating DHT with protein synthesis using both labelling techniques were combined, the curves were parallel and a strong correlation was noted between DHT and protein synthesis over a wide range of values (P less than 0.001).
  • (9) In choosing between various scanning techniques the factors to be considered include availability, cost, the type of equipment, the expertise of the medical and technical staff, and the inherent capabilities of the system.
  • (10) Past imaging techniques shown in the courtroom have made the conventional rules of evidence more difficult because of the different informational content and format required for presentation of these data.
  • (11) The strengths and weaknesses of each technique are described in this article.
  • (12) While stereology is the principal technique, particularly in its application to the parenchyma, other compartments such as the airways and vasculature demand modifications or different methods altogether.
  • (13) Standard nerve conduction techniques using constant measured distances were applied to evaluate the median, ulnar and radial nerves.
  • (14) The stopped-flow technique was used to measure the rate constants for the reactions between the oxidized forms of peroxidase with luminol and the following substrates: p-iodophenol, p-bromophenol, p-clorophenol, o-iodophenol, m-iodophenol, luciferin, and 2-iodo-6-hydroxybenzothiazole.
  • (15) The purpose of this paper is to discuss the potential for integrating surveillance techniques in reproductive epidemiology with geographic information system technology in order to identify populations at risk around hazardous waste sites.
  • (16) Here, we review the nature of the heart sound signal and the various signal-processing techniques that have been applied to PCG analysis.
  • (17) Chromatography and immunoassays are the two principal techniques used in research and clinical laboratories for the measurement of drug concentrations in biological fluids.
  • (18) Six of the patients were operated using the McIndoe and Bannister technique while on the other two the Tobin and Day technique was used.
  • (19) A review is presented concerning the development of new neuroimaging techniques in the last decade which have improved the diagnostic exploration of patients with spinal cord injuries, including studies of possible sequelae.
  • (20) How useful is the technique for evaluating therapeutic efficacy?