(1) Western blot analysis of these mitochondria using an antibody against carnitine palmitoyltransferase II purified from beef heart demonstrates a 68-kDa protein, which under ischemic conditions apparently is decreased by 2 kDa.
(2) We have developed a new procedure for the rapid preparation of undegraded total RNA from cultured cells for specific quantitation by dot blotting analysis.
(3) Only the approximately 2.7 kb mRNA species was visualized in Northern blots of total cellular and poly(A+) RNA isolated from cardiac ventricular muscle.
(4) In addition to performing Northern and Western blots for tyrosinase, tyrosine hydroxylase activity (THA) and dopa oxidase activity (DOA) were measured.
(5) After Western blot, 2 of the 5 protein bands of swine-cag (27 and 57 kD) and 3 of the 8 protein bands of human cag (27, 32, and 57 kD) reacted with the anti-Toxoplasma antibody used in the ELISA.
(6) Northern blot analysis with an 18-mer radiolabelled oligonucleotide, derived from an ALP specific cDNA clone, revealed a specific mRNA of about 700-800 nucleotides in HS-24 tumor cells.
(7) We have examined the pattern of bFGF expression during CNS development using protein immunoblot and RNA blot analyses.
(8) Therefore, we conclude this is a bovine DR beta-like pseudogene, BoDR beta I. Exon-containing regions have been used as probes in Southern blot analyses of bovine genomic DNA digested with EcoRI.
(9) Three distinct antigenic regions of bovine somatotropin (bST) were identified on the basis of the ability of a set of monoclonal antibodies to bind to proteolytic fragments and deletion variants of recombinant bST (rbST) in Western blot analyses.
(10) cDNA isolations, RNA, and genomic DNA blots confirm the existence and expression of two genes that produce indistinguishable SOD-4 proteins.
(11) We studied the feasibility of using RNA and DNA from autopsies for Northern and Southern blot analysis.
(12) Analysis of glutamate dehydrogenase (GDH) expression by enzyme assay and immunoblots, as well as Northern and dot blots of poly (A)+ RNA, in the deletion strains indicates that there are two upstream regulatory sequences that control the level of gene expression.
(13) The probe encoding LHCPII hybridizes to RNAs of 9.5 and 6.6 kb on northern blots of total RNA while the 3'-end probe hybridizes only to the 6.6 kb RNA.
(14) Enzyme activities were measured on nitrocellulose blots by using pure enzyme preparations as well as Triton X-100-solubilized membranes.
(15) The results indicated a very good comparability between the dot-blot assay and IF-tests, and this dot-blot method was ascertained as a simple and useful method for the scrub typhus serodiagnosis.
(16) An increase in cytoplasmic PRL mRNA content was evident in all the animals treated with estrogen as revealed with cytoplasmic dot blot analysis.
(17) All these strains produced an enterotoxic principle, antigenically related to cholera coli family of enterotoxins, as detected by latex agglutination and immuno-dot-blot tests.
(18) These alterations were not dependent on the prophage integration prior to curing, and no phage DNA was detected in cured cells by blot hybridisation.
(19) This study shows that the sensitivity and specificity of in situ hybridisation for the detection of EBV genomes in AIDS related lymphomas approaches that of Southern blotting, even when using routinely processed archival, paraffin wax embedded material.
(20) Western blot analysis revealed that the effect of PMA on U-373MG cells shows specificity in that GFA protein levels decline, while those of other major cytoskeletal proteins were unaltered.
Clotting
Definition:
(p. pr. & vb. n.) of Clot
Example Sentences:
(1) The agent present in the serum which causes dissolution of the fibrin clot was isolated and identified as pepsinogen.
(2) A cDNA library prepared from human placenta has been screened for sequences coding for factor XIIIa, the enzymatically active subunit of the factor XIII complex that stabilizes blood clots through crosslinking of fibrin molecules.
(3) Congenitally deficient plasmas were used as the substrate for the measurement of procoagulant activities in a one-stage clotting assay.
(4) The risk of total occlusion and clot formation in the renal artery after subintimal injection is high.
(5) After 30 min incubation, blood clotting was observed in all 8 experiments with heparin concentrations of 0 and 1 U per ml; in 3 of 8 with 2 U per ml; and in none with greater than or equal to 3 U per ml.
(6) Purpura fulminans is the cutaneous manifestation of acute activation of the clotting mechanism resulting in massive hemorrhage due to an intravascular consumption coagulopathy.
(7) Electron microscopy showed that the clots consist mainly of a suspension of individual fibers, in contrast to clots made from native fibrinogen, which are highly branched.
(8) In testing the hypothesis that Lp(a) can competitively inhibit plasma clot lysis mediated by plasmin, the present study shows that Lp(a) significantly enhanced plasma clot lysis mediated by streptokinase or t-PA.
(9) After adding 125I-labeled fibrinogen to plasma, clots were made by adding thrombin and calcium and were then resuspended in normal plasma containing various concentrations of JTPI-1.
(10) Hematoma clot weighing 10 grams was removed through emergency craniotomy, followed by external decompression.
(11) These data suggest that, in addition to platelet activation, abnormalities of blood clotting, and particularly reduction of antithrombin III, may play a role in the thrombotic tendency associated with homocystinuria.
(12) is related to the presence of adherent clots along cerebral arteries and when severe may lead to cerebral infarction.
(13) Certain of the schistosomes were covered with a dense mass of interconnected blood platelets resembling a temporary haemostatic plug but not a blood clot.
(14) Concanavalin A was employed to study the role of platelet membrane glycoproteins in platelet-fibrin interactions during clot formation.
(15) Venous thrombosis occurred in 7 patients (17.5%) in the Kabi 2165 group, including two high, potentially emboligenic, localizations (5%), and in 4 patients (10%) in the standard heparin group, including 2 potentially emboligenic clots (5%).
(16) The main objective of these experiments was to develop and characterize a new experimental model of venous thrombosis, and determine whether a combination of vascular wall damage (crushing with hemostat clamps) and prolonged stasis produced more reproducible clots than prolonged stasis per se.
(17) The assay shows strong correlation with the immunologic assay for factor XIII catalytic subunit a (r = 0.94), the factor XIII dansylcadaverine assay (r = 0.83), and the factor XIII clot solubility test.
(18) Unlike thrombin, the newly isolated kallikrein-like enzyme did not cause formation of a fibrin clot when fibrinogen was mixed with the enzyme.
(19) It has to be assumed that in calves with respiratory distress syndrome--in analogy to pulmonary immaturity--the blood clotting mechanism is not yet fully developed.
(20) Five other patients with water-insoluble paraproteins were tested; two were clot-inhibitory.