What's the difference between blotting and slotting?
Blotting
Definition:
(p. pr. & vb. n.) of Blot
Example Sentences:
(1) Western blot analysis of these mitochondria using an antibody against carnitine palmitoyltransferase II purified from beef heart demonstrates a 68-kDa protein, which under ischemic conditions apparently is decreased by 2 kDa.
(2) We have developed a new procedure for the rapid preparation of undegraded total RNA from cultured cells for specific quantitation by dot blotting analysis.
(3) Only the approximately 2.7 kb mRNA species was visualized in Northern blots of total cellular and poly(A+) RNA isolated from cardiac ventricular muscle.
(4) In addition to performing Northern and Western blots for tyrosinase, tyrosine hydroxylase activity (THA) and dopa oxidase activity (DOA) were measured.
(5) After Western blot, 2 of the 5 protein bands of swine-cag (27 and 57 kD) and 3 of the 8 protein bands of human cag (27, 32, and 57 kD) reacted with the anti-Toxoplasma antibody used in the ELISA.
(6) Northern blot analysis with an 18-mer radiolabelled oligonucleotide, derived from an ALP specific cDNA clone, revealed a specific mRNA of about 700-800 nucleotides in HS-24 tumor cells.
(7) We have examined the pattern of bFGF expression during CNS development using protein immunoblot and RNA blot analyses.
(8) Therefore, we conclude this is a bovine DR beta-like pseudogene, BoDR beta I. Exon-containing regions have been used as probes in Southern blot analyses of bovine genomic DNA digested with EcoRI.
(9) Three distinct antigenic regions of bovine somatotropin (bST) were identified on the basis of the ability of a set of monoclonal antibodies to bind to proteolytic fragments and deletion variants of recombinant bST (rbST) in Western blot analyses.
(10) cDNA isolations, RNA, and genomic DNA blots confirm the existence and expression of two genes that produce indistinguishable SOD-4 proteins.
(11) We studied the feasibility of using RNA and DNA from autopsies for Northern and Southern blot analysis.
(12) Analysis of glutamate dehydrogenase (GDH) expression by enzyme assay and immunoblots, as well as Northern and dot blots of poly (A)+ RNA, in the deletion strains indicates that there are two upstream regulatory sequences that control the level of gene expression.
(13) The probe encoding LHCPII hybridizes to RNAs of 9.5 and 6.6 kb on northern blots of total RNA while the 3'-end probe hybridizes only to the 6.6 kb RNA.
(14) Enzyme activities were measured on nitrocellulose blots by using pure enzyme preparations as well as Triton X-100-solubilized membranes.
(15) The results indicated a very good comparability between the dot-blot assay and IF-tests, and this dot-blot method was ascertained as a simple and useful method for the scrub typhus serodiagnosis.
(16) An increase in cytoplasmic PRL mRNA content was evident in all the animals treated with estrogen as revealed with cytoplasmic dot blot analysis.
(17) All these strains produced an enterotoxic principle, antigenically related to cholera coli family of enterotoxins, as detected by latex agglutination and immuno-dot-blot tests.
(18) These alterations were not dependent on the prophage integration prior to curing, and no phage DNA was detected in cured cells by blot hybridisation.
(19) This study shows that the sensitivity and specificity of in situ hybridisation for the detection of EBV genomes in AIDS related lymphomas approaches that of Southern blotting, even when using routinely processed archival, paraffin wax embedded material.
(20) Western blot analysis revealed that the effect of PMA on U-373MG cells shows specificity in that GFA protein levels decline, while those of other major cytoskeletal proteins were unaltered.
Slotting
Definition:
(n.) The act or process of making slots, or mortises.
Example Sentences:
(1) • Gone Girl picked for opening slot at New York film festival • We predict how Venice, Toronto and Telluride will split the 2014 world premieres
(2) When Question Time was moved to an earlier 9pm slot in May during the MPs' expenses scandal, a panel including Martin Bell, Ben Bradshaw and William Hague had 3.7 million viewers and a 17% share.
(3) McCall and her ad director, Stuart Taylor, have also managed to offer 'page dominance' to all but the smallest potential advertisers, meaning that big ads will not be diluted down by having smaller slots alongside them.
(4) DNA is isolated from synchronized populations of G1 and S phase cells, it is slot-blotted at the same DNA concentration(s) for each population, and it is hybridized with 32P-labeled DNA probes that are specific to the regions of interest.
(5) The final episode of I Own Britain's Best Home drew 400,000 and 2% for Five in the same time slot.
(6) Experiments were performed to measure velocities in front of six slot hoods.
(7) The issue was first raised by BBC2 controller Janice Hadlow and brought to the attention of the then BBC Vision director Jana Bennett – number two to BBC director general Mark Thompson – after the sitcom, which was planned for a post-9pm watershed slot, was moved to pre-watershed.
(8) Leroy Sané, a substitute, slotted in seamlessly on his debut.
(9) Standard gels, 200 mm wide with 20 sample slots have also been used.
(10) Findley darts round him and slots him beneath the advancing Ricketts.
(11) 27 August, 8pm Will Self The nearest the book festival circuit has to a rock star has three slots.
(12) The gastric factors controlling abundance of mRNA encoding the important neuropeptide, gastrin releasing peptide (GRP) in rat stomach, were examined by Northern and slot blot analysis.
(13) A complete 0.018-inch slot straight-wire appliance was used to align the teeth, close lower spaces, and detail the occlusion.
(14) It seemed that a gust of wind had dislodged part of the screen’s moorings leaving the visiting Leicester party, who had to negotiate a new take-off slot for their post-match flight back to East Midlands, looking unimpressed when they ventured to the touchline.
(15) FISH results on primary tumors were concordant with slot blot results on amplification and with immunohistochemical detection of overexpression.
(16) Eggs of southern corn rootworm (Diabrotica undecimpunctata howardi Barber) were subjected to electromagnetic energy at 2.45 GHz in slotted waveguide applicators to determine ovicidal threshold levels.
(17) When incubated with alpha-32P-labeled ribonucleoside triphosphates in vitro, nuclei isolated from haploid or diploid cells transcribed rRNA, tRNA, and mRNAs in a strand-specific manner, as shown by slot blot hybridization of the in vitro synthesized RNA to cloned genes encoding 5.8S, 18S and 28S rRNAs, tRNATyr, and GAL7, URA3, TY1 and HIS3 mRNAs.
(18) Herein, we describe the procedures for preparation and labeling of DNA probes and the principles that regulate dot, slot and Southern blot hybridization.
(19) It was considerably — and predictably — up on the audience who used to watch Norton in his old 10pm slot on BBC2, when it was typically watched by between 1 million and 1.5 million viewers.
(20) BBC1 slipped to second place in the slot behind a repeat of Martin Clunes drama Doc Martin, with 3.6 million viewers and an 18% share between 9pm and 11pm.