(1) Human milk proteins were electrophoretically separated and transferred to nitrocellulose membranes which were then blocked with bovine serum albumin, "BLOTTO", casein, or Tween 20.
(2) Alongside his notable use of marijuana, it is suggested that Otto uses heroin and LSD, and in one episode he appears in an Alcoholics Anonymous meeting where he introduces himself thus: “My name is Otto, I love to get blotto!” 5 | Vod ... from Fresh Meat Facebook Twitter Pinterest Zawe Ashton as Vod.
(3) We pity them for living out every aspect of their lives online, a practice that impairs their ability to get blotto on White Lightning on a dreary Friday night, due to the worry about the potentially very public consequences.
(4) A picture of Fraser – bloodied and blotto, his skull fractured – became one of the enduring images of the night, along with that of a baffled boy crying into his Ireland scarf as the violence flared around him.
(5) Turns out your blotto-drunk party pics and flirty co-worker messages have no commercial value whatsoever.
(6) For blocking, and as sample diluent, a modification of 'BLOTTO' (5% nonfat dry milk and 0.2% Tween 20) was most satisfactory.
(7) Overnight preincubation (approximately 12 hr at 2 C) of fresh sera diluted (1:10) in sample diluent containing 10% BLOTTO significantly reduced background and improved the detection of experimentally infected pigs by enhancing positive-negative discrimination.
(8) Modifications evaluated included blocking the antigen-coated ELISA plate with sample diluent containing 10% bovine serum albumin (BSA) or 10% nonfat milk powder (bovine lacto transfer optimizer or BLOTTO), diluting sera in sample diluent containing 10% BSA or 10% BLOTTO, and preincubating samples in sample diluent containing 10% BSA or 10% BLOTTO.
(9) Or the huge woolly knitted by Mother while blotto on morphine.
Pissed
Definition:
Example Sentences:
(1) Perhaps it’s the lot of people like my colleagues here in the centre and me to wrestle with our consciences, shed tears, lose sleep and try to make the best of a very bad, heart-breaking job and leave the rest of the world to party, get pissed and celebrate Christmas.
(2) In the most hard-hitting attack on the Labour leader by any of his MPs since Ukip squeezed the party’s vote in the Heywood and Middleton byelection, Field accused Miliband of “pissing while Rome burns”.
(3) We haven't changed that much, we still take the piss out of each other, there's an understanding there that hasn't gone away.
(4) Brand isn’t the messiah (or just a naughty boy, for that matter) and his message pisses off plenty of people.
(5) Then there's me and my buddy Ralph Garman , who does a daily radio show in LA, doing our entertainment podcast Hollywood Babble-On , which is basically just two guys who've worked in showbiz long enough to have informed opinions, sitting around taking the piss out of the entertainment industry.
(6) Or the story about how Rich was en route from Switzerland to Finland and had to order his jet to reverse course at 20,000ft to avoid being arrested by the FBI at Helsinki airport; or the secret tunnel he built between the 'Dallas building' and the Glashof restaurant opposite so he could slip out to lunch without fear of being assassinated; or the time he was held hostage in Azerbaijan while his captors considered whether or not to sell him to the Russians (who were allegedly pissed off with Rich for nicking their reserves of gold and other precious metals), or the rumours that Rich had slipped in and out of Britain and the US on numerous occasions under false passports.
(7) I haven’t even watched it again because I’m so pissed off about the first one, because I thought Manny could have made that fight much easier than he did.
(8) Of course, after Hitler got into power and Low started, beautifully, to take the piss, Low, along with his cartooning colleagues Illingworth, Vicky and even Heath Robinson, was placed on the Gestapo's deathlist.
(9) The catch is that the wine has been spiked with an extinguished cigarette, bogies, phlegm, piss and maggots; Ryle tackles it with vigour.
(10) Rather than open downstairs and piss off the council, I decided to take it more slowly,” he says.
(11) To my teenage self, who wanted to join Baader-Meinhof and rob banks hand-in-hand with my dead-eyed dream-date Magdalena Kopp, she was the ultimate Hollywood bad girl, always pissing off the right people.
(12) But I know they will complement my weaknesses and help me make better decisions because they will challenge me and, at times, that will piss me off because I want them to think like me and be as excited as me,” he says.
(13) Charly Rexach played for Barcelona between 1965 and 1981 and talks about winning the cup, "their cup", as a way of "really pissing [Real Madrid] off."
(14) The only advantage to being considered insane is, Shields says, that "people don't get as angry with you when you piss them off".
(15) "I sometimes got the feeling that Kurt enjoyed that I pissed people off.
(16) It was written about in the press, and taken the piss out of on other late-night shows.
(17) After the game we shake hands and everything else – what the hell, you’re going to get pissed off?
(18) The only response in these circumstances is to do what the Guardian did – throw your hands up and take the piss .
(19) He said: "The office said she's taking the piss here.
(20) I had phoned up all the national newspapers to say that “something big” was going to happen, and they were a bit pissed off because it wasn’t the dramatic stunt they thought it would be.