(1) Human milk proteins were electrophoretically separated and transferred to nitrocellulose membranes which were then blocked with bovine serum albumin, "BLOTTO", casein, or Tween 20.
(2) Alongside his notable use of marijuana, it is suggested that Otto uses heroin and LSD, and in one episode he appears in an Alcoholics Anonymous meeting where he introduces himself thus: “My name is Otto, I love to get blotto!” 5 | Vod ... from Fresh Meat Facebook Twitter Pinterest Zawe Ashton as Vod.
(3) We pity them for living out every aspect of their lives online, a practice that impairs their ability to get blotto on White Lightning on a dreary Friday night, due to the worry about the potentially very public consequences.
(4) A picture of Fraser – bloodied and blotto, his skull fractured – became one of the enduring images of the night, along with that of a baffled boy crying into his Ireland scarf as the violence flared around him.
(5) Turns out your blotto-drunk party pics and flirty co-worker messages have no commercial value whatsoever.
(6) For blocking, and as sample diluent, a modification of 'BLOTTO' (5% nonfat dry milk and 0.2% Tween 20) was most satisfactory.
(7) Overnight preincubation (approximately 12 hr at 2 C) of fresh sera diluted (1:10) in sample diluent containing 10% BLOTTO significantly reduced background and improved the detection of experimentally infected pigs by enhancing positive-negative discrimination.
(8) Modifications evaluated included blocking the antigen-coated ELISA plate with sample diluent containing 10% bovine serum albumin (BSA) or 10% nonfat milk powder (bovine lacto transfer optimizer or BLOTTO), diluting sera in sample diluent containing 10% BSA or 10% BLOTTO, and preincubating samples in sample diluent containing 10% BSA or 10% BLOTTO.
(9) Or the huge woolly knitted by Mother while blotto on morphine.
Tight
Definition:
() of Tie
() p. p. of Tie.
(superl.) Firmly held together; compact; not loose or open; as, tight cloth; a tight knot.
(superl.) Close, so as not to admit the passage of a liquid or other fluid; not leaky; as, a tight ship; a tight cask; a tight room; -- often used in this sense as the second member of a compound; as, water-tight; air-tight.
(superl.) Fitting close, or too close, to the body; as, a tight coat or other garment.
(superl.) Not ragged; whole; neat; tidy.
(superl.) Close; parsimonious; saving; as, a man tight in his dealings.
(superl.) Not slack or loose; firmly stretched; taut; -- applied to a rope, chain, or the like, extended or stretched out.
(superl.) Handy; adroit; brisk.
(superl.) Somewhat intoxicated; tipsy.
(superl.) Pressing; stringent; not easy; firmly held; dear; -- said of money or the money market. Cf. Easy, 7.
(v. t.) To tighten.
Example Sentences:
(1) Freshly isolated sarcoplasmic reticulum vesicles contain 0.05 mol of tightly bound ADP and 0.03 mol of tightly bound ATP per mol of Ca2+, Mg2+-ATPase (ATP phosphohydrolase, EC 3.6.1.3).
(2) The expression of the Pgp isoforms appears to be tightly regulated and, at least in some cells, under complex hormonal control.
(3) Whereas the tight junctions of endoneurial capillaries are known to prevent certain blood-borne substances from entering the endoneurium, it was not clear whether the permeability of the pulpal capillaries, which are distant from the nerve fibres, could affect the nerve fibre environment.
(4) The cells are predominantly monopolar, tightly packed, and are flattened at the outer border of the ring.
(5) These data indicate that topoisomerase I and RNA polymerase I are tightly complexed both in vivo and in vitro, and suggest a role for DNA topoisomerase I in the transcription of ribosomal genes.
(6) Immunofluorescence and immunoelectronmicroscopy experiments demonstrated that while tight junctions demarcate PAS-O distribution in confluent cultures, apical polarity could be established at low culture densities when cells could not form tight junctions with neighboring cells.
(7) Investigations have been made to determine the identity and binding characteristics of the pterins that are bound tightly to dihydrofolate reductases which are isolated from vertebrate sources by a well established procedure.
(8) At all times, a tight inverse correlation exists between ATP and IMP concentrations.
(9) Recently, a gene for ITD (DYT1) in a non-Jewish kindred was located on chromosome 9q32-34, with tight linkage to the gene encoding gelsolin (GSN).
(10) This study investigates the use of the incentive inspirometer to observe the effects of tight versus loose clothing on inhalation volume with 17 volunteer subjects.
(11) In contrast, interchange of the histones and tightly bound non-histone protein DNA complexes from hormone-withdrawn and estrogen-stimulated chromatins during reconstitution did not affect the level of mRNAOV sequences produced.
(12) Using microelectrodes and various microscopic techniques active Na+ absorption as well as K+ secretion has been localized to the principal cells, while Cl- absorption was found to proceed largely, though not exclusively, through the tight junctions between cells.
(13) The successful establishment of a postcrisis SV-40 T antigen transformed epithelial cell line, 1HAEo-, which retains tight junctions and vectorial ion transport, is described.
(14) The present investigation shows that the intramembranous proteins of tight and gap junctions are mobile structures within the fluid membrane.
(15) In contrast, after incubation with 0.5% DOC, the core microfilaments are no longer tightly bundled yet the lateral arms remain attached with a distinct 33-nm periodicity.
(16) The data collected by several approaches reveal that assembly and maturation of vaccinia involves a tightly coupled sequence of interrelated events including the assembly of the envelope, post-translational cleavage of several virion polypeptides, and induction of the core enzymes.
(17) The tight coupling between neuronal activity and oxidative energy metabolism forms the basis for the use of cytochrome oxidase as an endogenous metabolic marker for neurons.
(18) When using a nylon thread for the attachment of a pseudophakos to the iris, it may happen that the suture is slung tightly around the implant-lens.
(19) Some antibodies and other proteins bind tightly to nitrocellulose and dissociation of these proteins by Tween 20 is barely detectable.
(20) Independent experts warn that rumours and deliberate misinformation about the regime are rife, partly because it is impossible to verify or disprove most stories about the tightly controlled country's elite.