(1) In the first assay, we used a simple density separation technique to remove dense neutrophils (PMN) from suspensions of blood and of bone marrow cells prior to culture in semisolid agar.
(2) After 12 days, 100% of the cells terminally differentiate, and clonogenic assays in IL 3-containing semisolid media indicate that the whole population has irreversibly lost proliferative capability.
(3) Three short-term human melanoma cell lines were tested for sensitivity to human recombinant alpha-tumor necrosis factor (TNF) in a semisolid agar colony formation assay.
(4) A 2 h exposure to HIV-1 resulted in a progressive and significant reduction of viable CD34+ cell number in liquid cultures and of granulocyte-macrophage, erythroid and megakaryocytic progenitors in semisolid cultures.
(5) When SV-MSCs were cultured in semisolid medium, they formed colonies of blast-like cells that, when replated on plastic, resumed adherent growth.
(6) The effects of transforming growth factor beta 3 (TGF-beta 3) on growth in semisolid cultures of enriched hematopoietic progenitors derived from normal human marrow and blood were evaluated.
(7) A culture system of semisolid medium was able to detect colony-forming units of chondrocytes.
(8) The expression of HLA-DR antigenic determinants on human hematopoietic progenitor cells (HPC) capable of differentiating into mature blood cells, as determined in semisolid cultures, has been demonstrated previously (3-7).
(9) Their B cells also have a characteristically low cloning efficiency in semisolid agar cultures, where LPS does not seem to be required.
(10) Colony-Stimulating Factor (CSF) production by peripheral blood leukocytes of renal transplant recipients was assayed using semisolid agar culture.
(11) on the basis of semisolid motility and ornithine decarboxylase tests.
(12) A transformed cystinotic cell line which was recovered from the crisis stage was indistinguishable from its transformed precrisis parental cell strain in growth rate in media containing either 1% or 10% serum, cloning efficiency on plastic, in semisolid media, or upon confluent monolayers of normal skin fibroblasts, expression of SV40 T antigen, or production of virus.
(13) After gel filtration, the 30-kDa activity stimulated the growth of very large colonies in semisolid medium consisting mainly of granulocytes with the remainder being macrophages.
(14) Single cell cloning of FD-TCL cells in semisolid medium required the addition of exogenous conditioned medium.
(15) The influence of lithium chloride on the proliferation of normal granulocyte-macrophage progenitor cells (CFU-GM) and clonogenic blasts (CFU-L) from patients with acute myeloid leukaemia in the semisolid cultures in vitro was investigated.
(16) The expression of EBNA-1, EBNA-2,EBNA-3A,EBNA-Lp or LMP caused shortening of doubling time, increased saturation cell density, reduced serum dependence, anchorage-independent growth in semisolid agar and activation of c-myc.
(17) Comparative studies of clonogenic ability of twenty four lung cancer samples have been performed in semisolid agar in vitro and in diffusion chambers in vivo.
(18) Semisolid cloning systems are now available to detect the specific progenitor cells of neutrophilmacrophages, eosinophils, megakaryocytes and erythroid cells.
(19) Microplaque formation is rapid, reproducible, and easily quantitated, occurs at temperatures ranging from 33 to 40 degrees C, and does not require a semisolid overlay.
(20) Organisms grown in a liquid overlay on a semisolid slant (biphasic medium) showed slow logarithmic growth and the presence of chromatoid material.