(1) Using the monoclonal antibody Ki-67 that detects a cell proliferation-associated human nuclear antigen, incorporations of bromodeoxyuridine (Brud) and 3H-Thymidine, we studied the proliferative capacity of Reed-Sternberg (RS) cells in Hodgkin's cell lines L428, KM-H2, Holden, L540 and L591.
(2) And if there is a correlation with injury prevention and proved medical benefits, these may be valid reasons for allowing those systems into the technical area for medical staff, in future.” Brud suspects some devices may already be being used discreetly in some matches, although there was nothing surreptitious about Paris Saint-Germain striker Zlatan Ibrahimovic ’s use of his: two years ago, he managed to alarm both footballing authorities and fashion commentators after removing his shirt, following a friendly against Real Madrid, to reveal a compression vest , containing a GPS unit, that bore more than a passing resemblance to a sports bra.
(3) It’s very difficult to track, from the referee’s perspective,” says Lukas Brud, secretary of the IFAB, “and one of the reasons we decided to allow it is [because] we cannot stop players wearing tiny chips and devices if they really want to.
(4) Cell proliferation was examined directly by an increase in cell number and in individual cells by immunofluorescent detection of proliferating cell nuclear antigen (PCNA) and the incorporation of 5-bromo-2'-deoxyuridine (BrUd).
(5) In the presence of 1% serum, 10(-9) M PRL increased the incorporation of BrUd and resulted in a 3-fold increase in the doubling rate.
(6) The results revealed that in all cell lines, except L591, more than 55% of RS cells were found positive for Ki-67, and over 50% of them had Brud incorporation 5 days after cultivation.