(1) Using the monoclonal antibody Ki-67 that detects a cell proliferation-associated human nuclear antigen, incorporations of bromodeoxyuridine (Brud) and 3H-Thymidine, we studied the proliferative capacity of Reed-Sternberg (RS) cells in Hodgkin's cell lines L428, KM-H2, Holden, L540 and L591.
(2) And if there is a correlation with injury prevention and proved medical benefits, these may be valid reasons for allowing those systems into the technical area for medical staff, in future.” Brud suspects some devices may already be being used discreetly in some matches, although there was nothing surreptitious about Paris Saint-Germain striker Zlatan Ibrahimovic ’s use of his: two years ago, he managed to alarm both footballing authorities and fashion commentators after removing his shirt, following a friendly against Real Madrid, to reveal a compression vest , containing a GPS unit, that bore more than a passing resemblance to a sports bra.
(3) It’s very difficult to track, from the referee’s perspective,” says Lukas Brud, secretary of the IFAB, “and one of the reasons we decided to allow it is [because] we cannot stop players wearing tiny chips and devices if they really want to.
(4) Cell proliferation was examined directly by an increase in cell number and in individual cells by immunofluorescent detection of proliferating cell nuclear antigen (PCNA) and the incorporation of 5-bromo-2'-deoxyuridine (BrUd).
(5) In the presence of 1% serum, 10(-9) M PRL increased the incorporation of BrUd and resulted in a 3-fold increase in the doubling rate.
(6) The results revealed that in all cell lines, except L591, more than 55% of RS cells were found positive for Ki-67, and over 50% of them had Brud incorporation 5 days after cultivation.
Bud
Definition:
(n.) A small protuberance on the stem or branches of a plant, containing the rudiments of future leaves, flowers, or stems; an undeveloped branch or flower.
(n.) A small protuberance on certain low forms of animals and vegetables which develops into a new organism, either free or attached. See Hydra.
(v. i.) To put forth or produce buds, as a plant; to grow, as a bud does, into a flower or shoot.
(v. i.) To begin to grow, or to issue from a stock in the manner of a bud, as a horn.
(v. i.) To be like a bud in respect to youth and freshness, or growth and promise; as, a budding virgin.
(v. t.) To graft, as a plant with another or into another, by inserting a bud from the one into an opening in the bark of the other, in order to raise, upon the budded stock, fruit different from that which it would naturally bear.
Example Sentences:
(1) Serially sectioned rabbit foliate taste buds were examined with high voltage electron microscopy (HVEM) and computer-assisted, three-dimensional reconstruction.
(2) Small pieces of anterior and posterior quail wing-bud mesoderm (HH stages 21-23) were placed in in vitro culture for up to 3 days.
(3) They are capable of synthesis and accumulation of glycogen and responsible for its transfer to sites of more intense metabolism (growth, bud, blastema).
(4) Pupils who disrupt the learning of their classmates are dealt with firmly and, in many cases, a short suspension is an effective way of nipping bad behaviour in the bud."
(5) Tissue sections, taken from foliate and circumvallate papillae, generally revealed taste buds in which all cells were immunoreactive; however, occasionally some taste buds were found to contain highly reactive individual cells adjacent to non-reactive cells.
(6) They were formed by budding off from the cytoplasmic projections of the osteoblastic tumor cells.
(7) These antibodies were used to study the localization and synthesis of myosin heavy chain and tropomyosin in the limb buds of premetamorphic (stage VI-VII) tadpoles treated with triiodothyronine (T3) to induce metamorphosis.
(8) In contrast, sporoblasts and budding and free sporozoites in mature oocysts were labeled uniformly on the outer surfaces of their plasma membranes, indicating a uniform distribution of CS protein on these membranes.
(9) Other experiments further implicated actin in the budding process during virus maturation, as there appeared to be a specific association of actin in vitro only with nucleocapsids that have terminated RNA synthesis, which is presumably a prerequisite to budding.
(10) By the time the bud was half the diameter of the mother cell, it almost always bore a vacuole.
(11) The ICC assay demonstrated the production of infectious HIV-1 particles and budding of mature virions was observed by electron microscopy.
(12) We report now that the hormonal metabolite of vitamin D3, namely 1,25-dihydroxyvitamin D3, stimulates chondrogenesis in cultures of stage 24 chick embryo limb bud mesenchymal cells, as evidenced by morphologic changes as well as by increased transcription of collagen type II and core protein genes.
(13) Lysis ability was acquired by growth in (or transfer to) an osmotically stabilized environment, but only under conditions which permitted budding.
(14) Intralobar pulmonary sequestration has generally been considered a congenital malformation in which an accessory lung bud develops, is enveloped by normal lung, and retains its systemic arterial supply.
(15) Consequently mother cells can switch their mating type whereas bud cells cannot.
(16) At the former site the membrane overlying the bud showed an electron opaque thickening which imparted to the mature particle an asymmetrical appearance.
(17) Recently, cDNA clones encoding several bovine CKI isoforms have been sequenced that show high sequence identity to the HRR25 gene product of the budding yeast Saccharomyces cerevisiae; HRR25 is required for normal cellular growth, nuclear segregation, DNA repair, and meiosis.
(18) Budding "yeast-like organisms" that were consistent with Cryptococcus neoformans appeared in tissue specimens.
(19) This decrease in virus release appeared to be due to interference with the virus budding process due to antibody-mediated modulation of virus-induced cell surface antigens.
(20) Transforming growth factor beta (TGF-beta) was tested for its ability to stimulate a chemotactic response in Stage 24 embryonic chick limb bud mesenchymal cells and muscle-derived fibroblasts.