What's the difference between buffer and soften?

Buffer


Definition:

  • (n.) An elastic apparatus or fender, for deadening the jar caused by the collision of bodies; as, a buffer at the end of a railroad car.
  • (n.) A pad or cushion forming the end of a fender, which receives the blow; -- sometimes called buffing apparatus.
  • (n.) One who polishes with a buff.
  • (n.) A wheel for buffing; a buff.
  • (n.) A good-humored, slow-witted fellow; -- usually said of an elderly man.

Example Sentences:

  • (1) In contrast, resting cells of strain CHA750 produced five times less IAA in a buffer (pH 6.0) containing 1 mM-L-tryptophan than did resting cells of the wild-type, illustrating the major contribution of TSO to IAA synthesis under these conditions.
  • (2) The proportion of motile spermatozoa decreased with time at the same rate when samples were prepared in either HEPES or phosphate buffers.
  • (3) The third route was quantitated by its sensitivity to probenecid and its activity was increased in saline buffers and upon addition of glucose and was inhibited by oligomycin.
  • (4) The 0.1 M phosphate buffer eluant was electrophoretically heterogeneous and did not elicit the production of bactericidal antibodies in rabbits.
  • (5) In the same buffer a resonance marked L by Russu et al.
  • (6) The polymerization of dATP, dCTP, and dGTP onto the defined length initiator, d(pA)10, has been carried out in four buffers.
  • (7) Hyperosmolar buffer slightly increased the sensitivity and maximal response to methacholine as well as the cholinergic twitch to electric field stimulation.
  • (8) The present results using approximately 12% hemoglobin concentration in 0.1 M Bistris buffer at pD 7 and 27 degrees C with and without organic phosphate show that there is no significant line broadening on oxygenation (from 0 to 50% saturation) to affect the determination of the intensities or areas of these resonances.
  • (9) These results indicate that FMLP increased a pulmonary microvascular permeability in isolated buffer-perfused rabbit lungs that is PMN dependent and mediated by LT produced possibly by a result of ROS production.
  • (10) Although the brain AP50 is prominently phosphorylated by an endogenous protein kinase in isolated coated vesicle preparations, the neuronal AP50 was not detectably phosphorylated in intact cells as assessed by two-dimensional non-equilibrium pH gradient gel electrophoresis of labeled cells dissolved directly in SDS-containing buffers.
  • (11) In conclusion, the ability of distal tubules to establish a significant pH gradient will contribute to the titration of non bicarbonate buffers, i.e., to titratable acid formation.
  • (12) Studies were conducted in isolated, buffer-perfused rat lungs to determine if prostaglandin (PG) E1 attenuated pulmonary edema provoked by hydrogen peroxide (H2O2).
  • (13) One cellulase is buffer-soluble, the other buffer-insoluble but extractable with high salt concentrations.
  • (14) A pH 2.5 buffer was found to be the most effective solution for eluting gp41 from a 41-1S monoclonal antibody column.
  • (15) Furthermore, even the action of Lys-5 on the Pseudomonas OM was abolished when the assays were performed in the presence of 150 mM NaCl instead of the low-ionic strength buffer earlier used by investigators studying the effect of polycations on the Pseudomonas OM.
  • (16) The results suggest that the conversion of the HRP-TMB reaction product to an electron-dense form during osmication is intimately associated with the pH of the phosphate buffer and the total time of osmication.
  • (17) This combined process decreased by 63% the decimal reduction times for the heat treatment when the organism was suspended in buffer and by 43% when suspended in milk.
  • (18) The temperature-activated 4 to 5 S EBP transformation is found to be highly reproducible without loss of [3H]estradiol-binding activity in a buffer containing an excess of [3H]estradiol, 40 mM Tris, 1 mM dithiothreitol, and 1 M urea at pH 7.4.
  • (19) Incubation of freshly isolated rat hepatocytes with highly purified radiolabeled rat transferrin in weakly buffered medium in the presence of 10 mM ethanol resulted in a marked diminution of iron uptake by these cells, associated with a greater pH depression than in ethanol-free control studies.
  • (20) It was found that the use of a pH 9.6 buffer during the coating of ELISA plates led to the dissociation of virions into subunits which bound preferentially to the solid phase.

Soften


Definition:

  • (v. t.) To make soft or more soft.
  • (v. t.) To render less hard; -- said of matter.
  • (v. t.) To mollify; to make less fierce or intractable.
  • (v. t.) To palliate; to represent as less enormous; as, to soften a fault.
  • (v. t.) To compose; to mitigate; to assuage.
  • (v. t.) To make less harsh, less rude, less offensive, or less violent, or to render of an opposite quality.
  • (v. t.) To make less glaring; to tone down; as, to soften the coloring of a picture.
  • (v. t.) To make tender; to make effeminate; to enervate; as, troops softened by luxury.
  • (v. t.) To make less harsh or grating, or of a quality the opposite; as, to soften the voice.
  • (v. i.) To become soft or softened, or less rude, harsh, severe, or obdurate.

Example Sentences:

  • (1) The other trend involved softening from penetrant liquid absorption and a concomitant decrease in hardness.
  • (2) Golding said the government would not soften its stance on drug trafficking and it intended to use a proportion of revenues from its licensing authority to support a public education campaign to discourage pot-smoking by young people and mitigate public health consequences.
  • (3) Pathologically, there was diffuse incomplete softening of white matter in all cases.
  • (4) Aware that her press secretary, Bernard Ingham, a former labour correspondent for the Guardian who understood the range of attitudes within trade unions, had tried to soften the impression that she saw Kinnock as another General Galtieri [Argentina’s president during the Falklands war], the draft text tried to distinguish between unions, rival parties and what the final text (the one she actually delivered) called “an organised revolutionary minority” with their “outmoded Marxist dogma about class warfare”.
  • (5) Add the onion, cook for three minutes, stirring, until softened, then add the wine, sage, lemon peel, lemon juice and 150ml water.
  • (6) Welfare cuts are now becoming a matter of life or death | Letters Read more But government sources suggested the political pressures on Osborne, who has been criticised publicly by a series of Tory MPs, suggest he will act more flexibly and direct substantial resources to softening the impact of the cuts.
  • (7) Moisture on the skin was shown to increase the discharge to a standard stimulus, probably by its softening effect on the stratum corneum.
  • (8) The importance of R for cervical softening during pregnancy and its interaction with E near term and during parturition are discussed.
  • (9) He and Cameron have spent the week softening up opinion for huge benefit cuts in next week’s budget , due to focus on tax credits, largely paid to in-work, ”hardworking” families, victims of Britain’s swelling ranks of the under-paid.
  • (10) The method of aspiration with a standard electric operative aspirator should be used for evacuation of the softened brain matter.
  • (11) But he also suggested the administration was softening its commitment to the Minsk framework for a deal.
  • (12) In a casserole over a medium heat, fry the onions in the oil and butter for 5 minutes, to soften.
  • (13) Its lines soften, its edges fade; it shrinks into the raw cold from the river, more like a shrouded mountain than a castle built for kings.
  • (14) The wizened fish is hammered with a mallet to soften it so you can pull it off in strips to eat.
  • (15) The substitution of the softeners with deionisers solved this important and unusual clinical problem.
  • (16) Softening and elution are not sufficient for constriction, however, since high potassium, 2,4-dinitrophenol, and cyanide inhibited constriction without inhibiting the softening or elution of axoplasm.
  • (17) Ribotyping patterns of aeromonads recovered from well 1, detention basin, sand filter, softener, and distribution samples were compared with those of the five clinical isolates.
  • (18) By softening these insects in a detergent solution, however, it is possible to make most observations in the same way as on fresh material.
  • (19) His and Osborne's post-election "softening up" is returning to haunt them.
  • (20) But recently, their attitude has softened as they realise the importance of music to the island.