(1) For this, different detergents such as Triton X-100, CHAPS and n-octyl beta-D glucopyranoside were tested at various concentrations, durations and temperatures of incubation.
(2) At detergent concentrations above their critical micelle concentrations, C12E8 was also much more effective than CHAPS, suggesting that micelles are not involved.
(3) That’s plain wrong, has been for decades, and a clever chap like Nelson should know it.
(4) End-on views of G on virus clearly showed triangles consisting of three dots indicating the trimeric nature of native G. End-on views of CHAPS-isolated G showed very similar triangles confirming that, using this detergent, G was solubilized in its native trimeric structure.
(5) Previous investigations (El Mestikawy et al., J Neurochem 51: 1031-1040, 1988) have shown that 5-HT1A binding sites (R[5-HT1A]) solubilized by CHAPS from rat hippocampal membranes can be modulated by guanine nucleotides, as expected from their solubilization together with associated G regulatory proteins (G).
(6) Initially, peripheral polypeptides were removed from apically enriched vesicles by washing with alkaline buffer (pH 10.8) containing 2 mM CHAPS.
(7) The enzymes could be solubilized from the membrane fractions using CHAPS, and the detergent-soluble activity partially restored by addition of phospholipids.
(8) Furthermore, the effects of detergents other than CHAPS on hydrodynamic parameters and on [3H]TCDD binding to the receptor were studied.
(9) Cation exchange chromatography on carboxymethylcellulose-Sephadex with a starting buffer of pH 5 containing 2 mM CHAPS plus 20 mM beta-OG, followed by a pH 8 buffer, showed a very small OD peak at the void volume (P) and a second peak with about 95% of the protein (E).
(10) The binding of CHAPS to the SynChropak Propyl stationary phase and its effects on retention were found to be readily reversible.
(11) Leukotriene C4 (LTC4) synthase was solubilized from the microsomes of guinea-pig lung by the new procedures of a combination of 3-[3-cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS), digitonin and KCl.
(12) These treatments also caused an altered distribution of phosphorylated integrin between the CHAPS soluble and insoluble fractions.
(13) The ryanodine receptor protein of sheep cardiac muscle sarcoplasmic reticulum membranes functions as a ligand-regulated ion channel following solubilization with the zwitterionic detergent CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1- propane sulphonate); purification by density gradient centrifugation, reconstitution into proteo-liposomes and incorporation into planar phospholipid bilayers.
(14) The chap who assessed my brother was a physiotherapist,” she said.
(15) The augmentation of tone was endothelium-dependent as it did not occur following functional destruction of the endothelium by perfusion of the vascular bed with the detergent CHAPS (0.3%) for 150s.
(16) Isoelectric focusing in 2% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) without urea gives good results as does 2% Nonidet-P40 with 8 M urea.
(17) Sodium nitroprusside was approximately 200 times less potent than acetylcholine in the presence of the endothelium and was the only vasorelaxant to be active after destruction of the endothelium by perfusion with 0.3% CHAPS; in the absence of the endothelium it was 3.7 times more potent as a vasodilator than in its presence.
(18) Among three detergents tested, CHAPS is the best in preserving hormonal binding affinity and specificity.
(19) In addition, the 44% peak became increasingly resistant to the inhibitory effect of CHAPS.
(20) Optimal yield was obtained by pretreatment of whole M. pneumoniae cells with buffer containing 1% Chaps and subsequent extraction with octylglucosid at a detergent to protein ratio of 5 and at octylglycoside concentrations between 1.5 and 2%.
Clapped
Definition:
(imp. & p. p.) of Clap
Example Sentences:
(1) I think we are still trying to understand all that and I think that fits under the broader topic of social licence and what bringing in automation to an area does to that region as a whole, which we don’t quite know yet.” Could carbon farming be the answer for a 'clapped-out' Australia?
(2) Both the Labour and Conservative parties have constantly and repeatedly failed to honour promises they have made about reforming, cleaning, modernising our clapped-out system."
(3) Jan Krcmar observes: "Hang on a minute there, Drogba just clearly clapped his hands!
(4) When the news came through that all US personnel were uninjured, Manning's colleagues all cheered and clapped.
(5) And religious guru Asaram Bapu suggested that the victim was not blameless, asking provocatively: "Can one hand clap?"
(6) She excitedly described how all the women were singing and clapping as they waited together in a communal cell.
(7) The miner's wife, Siân James, is to his left, staring directly at him, clapping too, looking as though she cannot believe her eyes.
(8) "The two men high-five each other, clap their hands, and do what looks like an extraordinary dance of celebration that lasts for three minutes.
(9) The hour-long event at the gates of the city hall concluded with a two-minute "no silence" where participants whistled, shouted, clapped and played musical instruments.
(10) Bolt wrote: “(Note: part of the Q&A audience actually clapped Mallah.
(11) There's an extraordinary array of high performance models that can do almost anything, but there's also a lot of clapped-out old bangers from the former communist bloc that can leak, break down and possibly even explode.
(12) When you go out on stage and people clap you, that's a mood-altering experience.
(13) "The problem comes down to a whole range of clapped-out rules and arrangements.
(14) She might not clap that line but the truth is the audience know I’m being sincere in the fact I’m just literally saying what I think.
(15) The obtained CLAP values in five healthy subjects and five patients with chronic liver disease coincided well (r greater than 0.9994) with those generated by the use of an established method.
(16) My friends and I clapped,” said Rukhmini Puri, a history student, as she emerged with her friends from a cinema in Nehru Place in Delhi, the Indian capital.
(17) The protests were so effective at associating clapping with dissent that the traditional 3 July independence day military parade was held without applause with only the brass bands of the military puncturing the silence .
(18) The players came in last so that we could clap them – and then he came.
(19) The orchestra plays a march and they accompany with clapping and stamping."
(20) Eubank Senior’s clapping grew more insistent as the crowd began to boo, rightly so.