(1) For this, different detergents such as Triton X-100, CHAPS and n-octyl beta-D glucopyranoside were tested at various concentrations, durations and temperatures of incubation.
(2) At detergent concentrations above their critical micelle concentrations, C12E8 was also much more effective than CHAPS, suggesting that micelles are not involved.
(3) That’s plain wrong, has been for decades, and a clever chap like Nelson should know it.
(4) End-on views of G on virus clearly showed triangles consisting of three dots indicating the trimeric nature of native G. End-on views of CHAPS-isolated G showed very similar triangles confirming that, using this detergent, G was solubilized in its native trimeric structure.
(5) Previous investigations (El Mestikawy et al., J Neurochem 51: 1031-1040, 1988) have shown that 5-HT1A binding sites (R[5-HT1A]) solubilized by CHAPS from rat hippocampal membranes can be modulated by guanine nucleotides, as expected from their solubilization together with associated G regulatory proteins (G).
(6) Initially, peripheral polypeptides were removed from apically enriched vesicles by washing with alkaline buffer (pH 10.8) containing 2 mM CHAPS.
(7) The enzymes could be solubilized from the membrane fractions using CHAPS, and the detergent-soluble activity partially restored by addition of phospholipids.
(8) Furthermore, the effects of detergents other than CHAPS on hydrodynamic parameters and on [3H]TCDD binding to the receptor were studied.
(9) Cation exchange chromatography on carboxymethylcellulose-Sephadex with a starting buffer of pH 5 containing 2 mM CHAPS plus 20 mM beta-OG, followed by a pH 8 buffer, showed a very small OD peak at the void volume (P) and a second peak with about 95% of the protein (E).
(10) The binding of CHAPS to the SynChropak Propyl stationary phase and its effects on retention were found to be readily reversible.
(11) Leukotriene C4 (LTC4) synthase was solubilized from the microsomes of guinea-pig lung by the new procedures of a combination of 3-[3-cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS), digitonin and KCl.
(12) These treatments also caused an altered distribution of phosphorylated integrin between the CHAPS soluble and insoluble fractions.
(13) The ryanodine receptor protein of sheep cardiac muscle sarcoplasmic reticulum membranes functions as a ligand-regulated ion channel following solubilization with the zwitterionic detergent CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1- propane sulphonate); purification by density gradient centrifugation, reconstitution into proteo-liposomes and incorporation into planar phospholipid bilayers.
(14) The chap who assessed my brother was a physiotherapist,” she said.
(15) The augmentation of tone was endothelium-dependent as it did not occur following functional destruction of the endothelium by perfusion of the vascular bed with the detergent CHAPS (0.3%) for 150s.
(16) Isoelectric focusing in 2% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) without urea gives good results as does 2% Nonidet-P40 with 8 M urea.
(17) Sodium nitroprusside was approximately 200 times less potent than acetylcholine in the presence of the endothelium and was the only vasorelaxant to be active after destruction of the endothelium by perfusion with 0.3% CHAPS; in the absence of the endothelium it was 3.7 times more potent as a vasodilator than in its presence.
(18) Among three detergents tested, CHAPS is the best in preserving hormonal binding affinity and specificity.
(19) In addition, the 44% peak became increasingly resistant to the inhibitory effect of CHAPS.
(20) Optimal yield was obtained by pretreatment of whole M. pneumoniae cells with buffer containing 1% Chaps and subsequent extraction with octylglucosid at a detergent to protein ratio of 5 and at octylglycoside concentrations between 1.5 and 2%.
Cove
Definition:
(n.) A retired nook; especially, a small, sheltered inlet, creek, or bay; a recess in the shore.
(n.) A strip of prairie extending into woodland; also, a recess in the side of a mountain.
(n.) A concave molding.
(n.) A member, whose section is a concave curve, used especially with regard to an inner roof or ceiling, as around a skylight.
(v. t.) To arch over; to build in a hollow concave form; to make in the form of a cove.
(v. t.) To brood, cover, over, or sit over, as birds their eggs.
(n.) A boy or man of any age or station.
Example Sentences:
(1) Updated at 2.56pm GMT 12.51pm GMT They also think the worst is over at the Cove House Inn, according to Steven Morris.
(2) FIVE MORE FRENCH COASTAL GEMS Marseille grotto Facebook Twitter Pinterest Photograph: Alamy A 40-minute walk from Marseille’s Luminy university campus, Calanque de Sugiton, the most picturesque of the city’s rugged, limestone coves has blue-green waters, twisted pine trees and a narrow island-rock to swim out to known as Le Torpilleur.
(3) This paper reviews the first seven days of the Station's role at Anzac Cove, during which time this essentially inexperienced medical unit treated and evacuated an estimated 2700 wounded Australian and New Zealand soldiers.
(4) This is what we imagined: the becalmed beauty of the Whitsunday Passage, that spectacular collection of islands protectively nestled inside the Great Barrier Reef, safe from prevailing winds; bright blue languid days gliding over turquoise waters, taking turns at the tiller in our togs; finding our own private cove as the sun goes down; diving into warm pristine waters; the tinkling of intimate laughter; the fizz of champagne and the sizzle of prawns on the barbie.
(5) The transient decrease in left circumflex coronary artery blood flow during atrial contraction (atrial coves) was examined in open-chest, heart-blocked dogs.
(6) Right diastolid flow runoff, including the cove late in diastole, resembled left circumflex runoff.
(7) Port Gaverne , a little cove near Port Isaac always described as "quaint", is a good place to watch seals (and occasional basking sharks, dolphins and porpoises), go fishing or rummage in rock pools.
(8) A potholed gravel road runs to a campsite at the mouth of the Mattole river and from there you can wander south down the coast for 25 miles before you come to the next road, at Shelter Cove.
(9) The ceremony takes place at a black Catholic Church in the Prairie Hamlet of Frilot Cove: the priest imagines Collins arriving in heaven and resolving to 'take this place apart', before the Hail Mary is sung in French, and accordions play a zydeco standard entitled 'I'm Coming Home' as the coffin is laid in the ground.
(10) They landed on the beach at Anzac Cove at 11 a.m. on 25th April 1915, and remained on a 20 metre stretch of beach through eight months of the Gallipoli campaign.
(11) 'Something I think people will like is that it's a girl (not a boy) who is adventurous, strong and brave' said Bookworm88, in her review of Dead Man's Cove by Lauren St John 'What I loved most was the chance to read about children whose lives are very different from mine", wrote Lottielongshanks of Sky Hawk, by Gill Lewis We'd like to find out about more books that show different lifestyles and break the stereotypes.
(12) If history isn’t your thing, the park also offers plenty of coastal scenery, including eight miles of hiking trails to secluded coves.
(13) According to local boatmen, the Rothschilds use this military-style craft to whisk their guests at a speed of 50 knots directly from the airport to a corner of north-east Corfu where the secluded coves and remote luxury villas have become a discreet playground for the rich and powerful to mix business and pleasure.
(14) Fifteen dolphins were taken on Sunday from the cove via sling and transferred to the Taiji harbour sea pens and several captive facilities in Taiji, the group said on its Facebook page .
(15) "Our losses in 2012 were devastating," said Matt Schlapp, a consultant with Cove Strategies.
(16) Photograph: Steven Morris Across the road from the Cove House Inn, at Brandy Cottage, Shaun Souster was mopping out his porch after seawater poured in.
(17) steven morris (@stevenmorris20) They've reopened the storm shutters on the Cove House Inn.
(18) Marks & Spencer is calling on volunteers living near beaches such as Eastney (near Portsmouth), Exmouth (Devon), Chesil Cove (Dorset) and Hayle Towans (Cornwall) to help clean and survey the affected areas during its Big Beach Clean-up between 24 and 30 April.
(19) Porth Iago and Porth Ferin, near Aberdaron, Gwynedd Porth Iago beach, Wales Photograph: Rob Smith These two coves are separated by a short walk along one of the newest parts of the Wales Coast Path.
(20) Designated the casualty clearing hospital, it was called a Casualty Clearing Station on the beach at Anzac Cove in the Gallipoli campaign.