(1) For this, different detergents such as Triton X-100, CHAPS and n-octyl beta-D glucopyranoside were tested at various concentrations, durations and temperatures of incubation.
(2) At detergent concentrations above their critical micelle concentrations, C12E8 was also much more effective than CHAPS, suggesting that micelles are not involved.
(3) That’s plain wrong, has been for decades, and a clever chap like Nelson should know it.
(4) End-on views of G on virus clearly showed triangles consisting of three dots indicating the trimeric nature of native G. End-on views of CHAPS-isolated G showed very similar triangles confirming that, using this detergent, G was solubilized in its native trimeric structure.
(5) Previous investigations (El Mestikawy et al., J Neurochem 51: 1031-1040, 1988) have shown that 5-HT1A binding sites (R[5-HT1A]) solubilized by CHAPS from rat hippocampal membranes can be modulated by guanine nucleotides, as expected from their solubilization together with associated G regulatory proteins (G).
(6) Initially, peripheral polypeptides were removed from apically enriched vesicles by washing with alkaline buffer (pH 10.8) containing 2 mM CHAPS.
(7) The enzymes could be solubilized from the membrane fractions using CHAPS, and the detergent-soluble activity partially restored by addition of phospholipids.
(8) Furthermore, the effects of detergents other than CHAPS on hydrodynamic parameters and on [3H]TCDD binding to the receptor were studied.
(9) Cation exchange chromatography on carboxymethylcellulose-Sephadex with a starting buffer of pH 5 containing 2 mM CHAPS plus 20 mM beta-OG, followed by a pH 8 buffer, showed a very small OD peak at the void volume (P) and a second peak with about 95% of the protein (E).
(10) The binding of CHAPS to the SynChropak Propyl stationary phase and its effects on retention were found to be readily reversible.
(11) Leukotriene C4 (LTC4) synthase was solubilized from the microsomes of guinea-pig lung by the new procedures of a combination of 3-[3-cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS), digitonin and KCl.
(12) These treatments also caused an altered distribution of phosphorylated integrin between the CHAPS soluble and insoluble fractions.
(13) The ryanodine receptor protein of sheep cardiac muscle sarcoplasmic reticulum membranes functions as a ligand-regulated ion channel following solubilization with the zwitterionic detergent CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1- propane sulphonate); purification by density gradient centrifugation, reconstitution into proteo-liposomes and incorporation into planar phospholipid bilayers.
(14) The chap who assessed my brother was a physiotherapist,” she said.
(15) The augmentation of tone was endothelium-dependent as it did not occur following functional destruction of the endothelium by perfusion of the vascular bed with the detergent CHAPS (0.3%) for 150s.
(16) Isoelectric focusing in 2% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) without urea gives good results as does 2% Nonidet-P40 with 8 M urea.
(17) Sodium nitroprusside was approximately 200 times less potent than acetylcholine in the presence of the endothelium and was the only vasorelaxant to be active after destruction of the endothelium by perfusion with 0.3% CHAPS; in the absence of the endothelium it was 3.7 times more potent as a vasodilator than in its presence.
(18) Among three detergents tested, CHAPS is the best in preserving hormonal binding affinity and specificity.
(19) In addition, the 44% peak became increasingly resistant to the inhibitory effect of CHAPS.
(20) Optimal yield was obtained by pretreatment of whole M. pneumoniae cells with buffer containing 1% Chaps and subsequent extraction with octylglucosid at a detergent to protein ratio of 5 and at octylglycoside concentrations between 1.5 and 2%.
Cracked
Definition:
(imp. & p. p.) of Crack
(a.) Coarsely ground or broken; as, cracked wheat.
(a.) Crack-brained.
Example Sentences:
(1) They’re no crack force either; many are rather portly!
(2) While superheroes like “superman” (21st in SplashData’s 2014 rankings) and “batman” (24th) may be popular choices for passwords, the results if they are cracked could be anything other than super – and users will only have themselves to blame.
(3) However, we have observed cracks on the Dacron fibers, fiber fracture, fiber protrusion, and poor attachment to the diaphragm, which can cause potentially disastrous complications.
(4) But instead, he is going to crack under public anger over the huge amounts senior bankers have been paying themselves.
(5) It is found that, in contrast to most metallic materials yet in keeping with many ceramics, there are no distinct fracture morphologies in pyro-carbons which are characteristic of a specific mode of loading; fracture surfaces appear to be identical for both catastrophic and subcritical crack growth under either sustained or cyclic loading.
(6) Iowa senator Chuck Grassley, the Republican who chairs the Senate judiciary committee, introduced legislation on Tuesday that would crack down on jurisdictions that provide safe harbor for undocumented migrants by withholding some federal funding for state and local entities if they decline to cooperate with the government on the holding or transferring of undocumented migrants with criminal records.
(7) In a single letter in February 2005, Charles urged a badger cull to prevent the spread of bovine tuberculosis – damning opponents to the cull as “intellectually dishonest”; lobbied for his preferred person to be appointed to crack down on the mistreatment of farmers by supermarkets; proposed his own aide to brief Downing Street on the design of new hospitals; and urged Blair to tackle an EU directive limiting the use of herbal alternative medicines in the UK.
(8) On second impacts, the GSI rose considerably because the shell and liner of the DH-151 cracked and the suspension of the "141" stretched during the first blow.
(9) 9.18pm: The Ohio Democrat Dennis Kucinich has a crack, and he's taking no prisoners: "We've heard Mr Toyoda say that Toyota grew to fast.
(10) Here come the Dodgers for another crack at it in the second.
(11) That led to the second breakthrough, as the once formidable laws of omerta - silence punishable by death - cracked.
(12) The passengers were then flown to an Australian icebreaker, the Aurora Australis, which had cracked through ice floes and was now sailing towards Australia's Casey research base.
(13) SEM of the resulting surface showed rounded fragments of enamel rods, enamel melting, cracks, and smooth-edged voids.
(14) According to the NYPD commissioner, Bill Bratton, whose voice almost cracked with emotion as he addressed the media on Saturday evening , the “digital warning poster” featuring a picture of Brinsley and his whereabouts arrived at the data centre at 2.47pm.
(15) As subcritical crack velocities under cyclic loading were found to be many orders of magnitude faster than those measured under equivalent monotonic loads and to occur at typically 45% lower stress-intensity levels, cyclic fatigue in pyrolytic carbon-coated graphite is reasoned to be a vital consideration in the design and life-prediction procedures of prosthetic devices manufactured from this material.
(16) It is hence impossible to wiretap, intercept or crack the information transmitted through it,” Xinhua reported after Tuesday’s launch.
(17) He just never dreamed it would be life without parole.” Obama reduces sentences of 46 inmates convicted of nonviolent drug crimes Read more As his sister put it, Bennett “got caught up” in a five-man drug ring run by an old friend, John Hansley, to pay for his addiction to crack.
(18) Spencer has now heard that Andy, who got the boat remember, has been cracking on to Louise, even though Jamie warned him it would be like jumping into a polar bear's nest.
(19) "I urge both the monks and the lay Tibetans of the area not to do anything that might be used as a pretext by the local authorities to massively crack down on them.
(20) Of 26 patients treated to date, 16 have been crack cocaine users.