(1) For this, different detergents such as Triton X-100, CHAPS and n-octyl beta-D glucopyranoside were tested at various concentrations, durations and temperatures of incubation.
(2) At detergent concentrations above their critical micelle concentrations, C12E8 was also much more effective than CHAPS, suggesting that micelles are not involved.
(3) That’s plain wrong, has been for decades, and a clever chap like Nelson should know it.
(4) End-on views of G on virus clearly showed triangles consisting of three dots indicating the trimeric nature of native G. End-on views of CHAPS-isolated G showed very similar triangles confirming that, using this detergent, G was solubilized in its native trimeric structure.
(5) Previous investigations (El Mestikawy et al., J Neurochem 51: 1031-1040, 1988) have shown that 5-HT1A binding sites (R[5-HT1A]) solubilized by CHAPS from rat hippocampal membranes can be modulated by guanine nucleotides, as expected from their solubilization together with associated G regulatory proteins (G).
(6) Initially, peripheral polypeptides were removed from apically enriched vesicles by washing with alkaline buffer (pH 10.8) containing 2 mM CHAPS.
(7) The enzymes could be solubilized from the membrane fractions using CHAPS, and the detergent-soluble activity partially restored by addition of phospholipids.
(8) Furthermore, the effects of detergents other than CHAPS on hydrodynamic parameters and on [3H]TCDD binding to the receptor were studied.
(9) Cation exchange chromatography on carboxymethylcellulose-Sephadex with a starting buffer of pH 5 containing 2 mM CHAPS plus 20 mM beta-OG, followed by a pH 8 buffer, showed a very small OD peak at the void volume (P) and a second peak with about 95% of the protein (E).
(10) The binding of CHAPS to the SynChropak Propyl stationary phase and its effects on retention were found to be readily reversible.
(11) Leukotriene C4 (LTC4) synthase was solubilized from the microsomes of guinea-pig lung by the new procedures of a combination of 3-[3-cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS), digitonin and KCl.
(12) These treatments also caused an altered distribution of phosphorylated integrin between the CHAPS soluble and insoluble fractions.
(13) The ryanodine receptor protein of sheep cardiac muscle sarcoplasmic reticulum membranes functions as a ligand-regulated ion channel following solubilization with the zwitterionic detergent CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1- propane sulphonate); purification by density gradient centrifugation, reconstitution into proteo-liposomes and incorporation into planar phospholipid bilayers.
(14) The chap who assessed my brother was a physiotherapist,” she said.
(15) The augmentation of tone was endothelium-dependent as it did not occur following functional destruction of the endothelium by perfusion of the vascular bed with the detergent CHAPS (0.3%) for 150s.
(16) Isoelectric focusing in 2% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) without urea gives good results as does 2% Nonidet-P40 with 8 M urea.
(17) Sodium nitroprusside was approximately 200 times less potent than acetylcholine in the presence of the endothelium and was the only vasorelaxant to be active after destruction of the endothelium by perfusion with 0.3% CHAPS; in the absence of the endothelium it was 3.7 times more potent as a vasodilator than in its presence.
(18) Among three detergents tested, CHAPS is the best in preserving hormonal binding affinity and specificity.
(19) In addition, the 44% peak became increasingly resistant to the inhibitory effect of CHAPS.
(20) Optimal yield was obtained by pretreatment of whole M. pneumoniae cells with buffer containing 1% Chaps and subsequent extraction with octylglucosid at a detergent to protein ratio of 5 and at octylglycoside concentrations between 1.5 and 2%.
Dry
Definition:
(superl.) Free from moisture; having little humidity or none; arid; not wet or moist; deficient in the natural or normal supply of moisture, as rain or fluid of any kind; -- said especially: (a) Of the weather: Free from rain or mist.
(superl.) Of vegetable matter: Free from juices or sap; not succulent; not green; as, dry wood or hay.
(superl.) Of animals: Not giving milk; as, the cow is dry.
(superl.) Of persons: Thirsty; needing drink.
(superl.) Of the eyes: Not shedding tears.
(superl.) Of certain morbid conditions, in which there is entire or comparative absence of moisture; as, dry gangrene; dry catarrh.
(superl.) Destitute of that which interests or amuses; barren; unembellished; jejune; plain.
(superl.) Characterized by a quality somewhat severe, grave, or hard; hence, sharp; keen; shrewd; quaint; as, a dry tone or manner; dry wit.
(superl.) Exhibiting a sharp, frigid preciseness of execution, or the want of a delicate contour in form, and of easy transition in coloring.
(a.) To make dry; to free from water, or from moisture of any kind, and by any means; to exsiccate; as, to dry the eyes; to dry one's tears; the wind dries the earth; to dry a wet cloth; to dry hay.
(v. i.) To grow dry; to become free from wetness, moisture, or juice; as, the road dries rapidly.
(v. i.) To evaporate wholly; to be exhaled; -- said of moisture, or a liquid; -- sometimes with up; as, the stream dries, or dries up.
(v. i.) To shrivel or wither; to lose vitality.
Example Sentences:
(1) Maximal yields of lipid and aflatoxin were obtained with 30% glucose, whereas mold growth, expressed as dry weight, was maximal when the medium contained 10% glucose.
(2) A 24-h test trial employing a dry target demonstrated a robust memory for the training manifested in passive avoidance behavior.
(3) Over the years the farm dams filled less frequently while the suburbs crept further into the countryside, their swimming pools oblivious to the great drying.
(4) It was shown that gradual recovery of spike wave patterns occurred from initial water swallowing to successive dry swalllowing.
(5) Mucosal drying medications and senile salivary gland atrophy seemed to contribute to the high frequency of sicca in this population with a lesser proportion of the subjects demonstrating previously undiagnosed Sjögren's and possible Sjögren's syndrome.
(6) Where the guanine content was more than or equal to 0.25% in the dry dust, mite numbers were higher than 10 mites per 0.1 g dust in 43 of the 44 samples.
(7) Reconstituted freeze dried allogeneic skin grafts contained virtually no blood, a phenomenon possibly analogous to the 'no reflow' phenomenon of microsurgery.
(8) In Humbo in Ethiopia , FMNR has re-greened 2,800 hectares: springs, dry for 30 years, are flowing again.
(9) 54% of patients in the rainy season were ELISA positive for RSV compared to 8.8% during the dry season.
(10) This study compares the effects of 60 minutes of ischemic arrest with profound topical hypothermia (10 dogs) on myocardial (1) blood flow and distribution (microspheres), (2) metabolism (oxygen and lactate), (3) water content (wet to dry weights), (4) compliance (intraventricular balloon), and (5) performance (isovolumetric function curves) with 180 minutes of cardiopulmonary bypass with the heart in the beating empty state (seven dogs).
(11) Healthbars such as Nakd fit this category and promise to deliver one of your five a day, based on the quantity of freeze-dried date paste used.
(12) Freeze-dried mannitol preparations were shown to be of a crystalline nature.
(13) The dried-specimen-teasing method appears useful, because of the ease of preparation of the specimens, its reproducibility, and the degree of visibility and preservation of cell surface structures and intraclonal relationships.
(14) The parameters of LES relaxation for both wet and dry swallows were similar using either a carefully placed single recording orifice or a Dent sleeve.
(15) The concentration of prey and the ciliate mean cell volume, dry weight, and number per milliliter were determined at known growth rates.
(16) The first stop in this arid place of poor farms and orchards clinging to the dry soil is Rafah, cut off by the border from its Palestinian counterpart.
(17) Percentage of dry tissue and protein concentration increased in parallel during the whole period.
(18) A clinical investigation was made between workers exposed to dried sewage sludge dust and age matched controls not exposed.
(19) During suction a flow of cold, dry room air replaces the warm, moist cavity air, causing cooling both directly and by vaporization of water.
(20) Patients with complaints of dry eyes and dry mouth but with no objective abnormalities served as control group.