(1) For this, different detergents such as Triton X-100, CHAPS and n-octyl beta-D glucopyranoside were tested at various concentrations, durations and temperatures of incubation.
(2) At detergent concentrations above their critical micelle concentrations, C12E8 was also much more effective than CHAPS, suggesting that micelles are not involved.
(3) That’s plain wrong, has been for decades, and a clever chap like Nelson should know it.
(4) End-on views of G on virus clearly showed triangles consisting of three dots indicating the trimeric nature of native G. End-on views of CHAPS-isolated G showed very similar triangles confirming that, using this detergent, G was solubilized in its native trimeric structure.
(5) Previous investigations (El Mestikawy et al., J Neurochem 51: 1031-1040, 1988) have shown that 5-HT1A binding sites (R[5-HT1A]) solubilized by CHAPS from rat hippocampal membranes can be modulated by guanine nucleotides, as expected from their solubilization together with associated G regulatory proteins (G).
(6) Initially, peripheral polypeptides were removed from apically enriched vesicles by washing with alkaline buffer (pH 10.8) containing 2 mM CHAPS.
(7) The enzymes could be solubilized from the membrane fractions using CHAPS, and the detergent-soluble activity partially restored by addition of phospholipids.
(8) Furthermore, the effects of detergents other than CHAPS on hydrodynamic parameters and on [3H]TCDD binding to the receptor were studied.
(9) Cation exchange chromatography on carboxymethylcellulose-Sephadex with a starting buffer of pH 5 containing 2 mM CHAPS plus 20 mM beta-OG, followed by a pH 8 buffer, showed a very small OD peak at the void volume (P) and a second peak with about 95% of the protein (E).
(10) The binding of CHAPS to the SynChropak Propyl stationary phase and its effects on retention were found to be readily reversible.
(11) Leukotriene C4 (LTC4) synthase was solubilized from the microsomes of guinea-pig lung by the new procedures of a combination of 3-[3-cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS), digitonin and KCl.
(12) These treatments also caused an altered distribution of phosphorylated integrin between the CHAPS soluble and insoluble fractions.
(13) The ryanodine receptor protein of sheep cardiac muscle sarcoplasmic reticulum membranes functions as a ligand-regulated ion channel following solubilization with the zwitterionic detergent CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1- propane sulphonate); purification by density gradient centrifugation, reconstitution into proteo-liposomes and incorporation into planar phospholipid bilayers.
(14) The chap who assessed my brother was a physiotherapist,” she said.
(15) The augmentation of tone was endothelium-dependent as it did not occur following functional destruction of the endothelium by perfusion of the vascular bed with the detergent CHAPS (0.3%) for 150s.
(16) Isoelectric focusing in 2% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) without urea gives good results as does 2% Nonidet-P40 with 8 M urea.
(17) Sodium nitroprusside was approximately 200 times less potent than acetylcholine in the presence of the endothelium and was the only vasorelaxant to be active after destruction of the endothelium by perfusion with 0.3% CHAPS; in the absence of the endothelium it was 3.7 times more potent as a vasodilator than in its presence.
(18) Among three detergents tested, CHAPS is the best in preserving hormonal binding affinity and specificity.
(19) In addition, the 44% peak became increasingly resistant to the inhibitory effect of CHAPS.
(20) Optimal yield was obtained by pretreatment of whole M. pneumoniae cells with buffer containing 1% Chaps and subsequent extraction with octylglucosid at a detergent to protein ratio of 5 and at octylglycoside concentrations between 1.5 and 2%.
Flaky
Definition:
(a.) Consisting of flakes or of small, loose masses; lying, or cleaving off, in flakes or layers; flakelike.
Example Sentences:
(1) She stayed calm during the upsetting search that led to Cynthia, who turned out to be flaky, chain-smoking and white (played by Brenda Blethyn).
(2) Corbyn to complain to MoD about army chief's ‘political interference’ Read more Meanwhile, Jeremy Corbyn’s political mis-steps over the past 10 days have allowed his views to be dismissed as flaky and irresponsible – even where he is right, as in his warnings about kneejerk responses to terrorist attacks and, indeed, in his Armistice Day strictures about the requirement for the top brass to stay out of politics .
(3) Patients with acute or chronic liver disease often suffer from dry, itchy and flaky skin.
(4) It's paid for on a wing and a prayer ... George Osborne's savings are so flaky, he's admitted he doesn't even know which department is going to pay what."
(5) We observed the labelling of 4-8 nm wide amyloid fibrils in the plaque cores and of extracellular electrondense, flaky and irregularly distributed material in the preamyloid deposits.
(6) Labour MPs tell me that the position of their traditional supporters is “flaky”.
(7) They are firmer and less flaky than Cornish pasties and don't break, making them the perfect picnic food.
(8) The business plan of the bridge promoters is a bit flaky, I think, based on assumptions, and the public purse may have to underwrite other costs we don’t know about yet.
(9) For the dressing 1 tbsp cider or white-wine vinegar 3 tbsp olive oil Flaky sea salt and freshly ground black pepper For the salad A couple of handfuls rocket leaves 80g semi-soft blue cheese 6 dates, pitted and sliced 50g hazelnuts, toasted and roughly chopped Whisk together the vinegar and oil until you have a creamy emulsion, then pour a tablespoon into the bottom of a bowl.
(10) And while DeMerit has probably chosen the right time to call it a day, Carl Robinson would have loved at least another year of peak production from his captains as he tries to build some solidity into a talented but sometimes flaky Vancouver team.
(11) After a promising start it appears this press conference has degenerated into its usual cocoon of flaky stuff.
(12) Private landlords stop renting to “flaky” benefit recipients, social landlords’ rental income dries up and (because they are forced to renege on their own borrowing commitments) stop building new affordable housing.
(13) The electron micrographic scanning of specimens of calculi revealed the following matrix morphologies: (i) flaky, (ii) serrated, (iii) perforated (globular), (iv) hollow tubular, (v) fibrillar, (vi) cylindrical, and (vii) pear-drop shaped.
(14) As a revertant of this character, a flaky mutant was isolated, showing a heavy flocculation during growth on liquid medium and resistance to catabolite repression for maltase, alpha-methyl-glucosidase, invertase, and succinate dehydrogenase.
(15) She also failed to write her title correctly on a whiteboard, was filmed painting her "flaky" nails and compared the force to a tin of paint that she wanted to "prise" open.
(16) He is regularly described as "brilliant", "sophisticated", "not flaky", "operationally effective".
(17) Before that people thought it was just flaky, feelgood stuff," says Richard Jolly, who worked for the United Nations Children's Fund and is writing a history of the UN.
(18) They produce a dirty white almost flaky odoriferous substance which clings to the hairs of the area and is easily rubbed off for marking territorial areas as well as for marking females during mating.
(19) "I am absolutely not prepared to do that if other people take a flaky pick and choose approach to what we agreed," Clegg says when asked whether he would support the reforms when Cameron presses ahead with a vote in the Commons.
(20) The wage penalty arising from flaky sub-degree-level qualifications – a longstanding weakness – would rise, as would the premium for those who can combine rigorous analytical thinking with creativity.