(v. t.) To lay on or impose, as a load, tax, or burden; to load; to fill.
(v. t.) To lay on or impose, as a task, duty, or trust; to command, instruct, or exhort with authority; to enjoin; to urge earnestly; as, to charge a jury; to charge the clergy of a diocese; to charge an agent.
(v. t.) To lay on, impose, or make subject to or liable for.
(v. t.) To fix or demand as a price; as, he charges two dollars a barrel for apples.
(v. t.) To place something to the account of as a debt; to debit, as, to charge one with goods. Also, to enter upon the debit side of an account; as, to charge a sum to one.
(v. t.) To impute or ascribe; to lay to one's charge.
(v. t.) To accuse; to make a charge or assertion against (a person or thing); to lay the responsibility (for something said or done) at the door of.
(v. t.) To place within or upon any firearm, piece of apparatus or machinery, the quantity it is intended and fitted to hold or bear; to load; to fill; as, to charge a gun; to charge an electrical machine, etc.
(v. t.) To ornament with or cause to bear; as, to charge an architectural member with a molding.
(v. t.) To assume as a bearing; as, he charges three roses or; to add to or represent on; as, he charges his shield with three roses or.
(v. t.) To call to account; to challenge.
(v. t.) To bear down upon; to rush upon; to attack.
(v. i.) To make an onset or rush; as, to charge with fixed bayonets.
(v. i.) To demand a price; as, to charge high for goods.
(v. i.) To debit on an account; as, to charge for purchases.
(v. i.) To squat on its belly and be still; -- a command given by a sportsman to a dog.
(v. t.) A load or burder laid upon a person or thing.
(v. t.) A person or thing commited or intrusted to the care, custody, or management of another; a trust.
(v. t.) Custody or care of any person, thing, or place; office; responsibility; oversight; obigation; duty.
(v. t.) Heed; care; anxiety; trouble.
(v. t.) Harm.
(v. t.) An order; a mandate or command; an injunction.
(v. t.) An address (esp. an earnest or impressive address) containing instruction or exhortation; as, the charge of a judge to a jury; the charge of a bishop to his clergy.
(v. t.) An accusation of a wrong of offense; allegation; indictment; specification of something alleged.
(v. t.) Whatever constitutes a burden on property, as rents, taxes, lines, etc.; costs; expense incurred; -- usually in the plural.
(v. t.) The price demanded for a thing or service.
(v. t.) An entry or a account of that which is due from one party to another; that which is debited in a business transaction; as, a charge in an account book.
(v. t.) That quantity, as of ammunition, electricity, ore, fuel, etc., which any apparatus, as a gun, battery, furnace, machine, etc., is intended to receive and fitted to hold, or which is actually in it at one time
(v. t.) The act of rushing upon, or towards, an enemy; a sudden onset or attack, as of troops, esp. cavalry; hence, the signal for attack; as, to sound the charge.
(v. t.) A position (of a weapon) fitted for attack; as, to bring a weapon to the charge.
(v. t.) A soft of plaster or ointment.
(v. t.) A bearing. See Bearing, n., 8.
(n.) Thirty-six pigs of lead, each pig weighing about seventy pounds; -- called also charre.
(n.) Weight; import; value.
Example Sentences:
(1) The Wales international and Port Vale defender Clayton McDonald both admitted having sex with the victim, – McDonald was found not guilty of the same charge.
(2) Yet the Tory promise of fiscal rectitude prevailed in England Alexander had been in charge of Labour’s election strategy, but he could not strategise a victory over a 20-year-old Scottish nationalist who has not yet taken her finals.
(3) The previous year, he claimed £1,415 for two new sofas, made two separate claims of £230 and £108 for new bed linen, charged £86 for a new kettle and kitchen utensils and made two separate claims, of £65 and £186, for replacement glasses and crockery.
(4) They had allegedly agreed that Younous would not be charged with any crime upon his arrival there and that he would not be detained in Morocco for longer than 72 hours.
(5) I’m not in charge of it but he’s stood up and presented that, and when Jenny, you know, criticised it, or raised some issues about grandparent carers – 3,700 of them he calculated – he said “Let’s sit down”.
(6) But the wounding charge in 2010 has become Brown's creation of a structural hole in the budget, more serious than the cyclical hit which the recession made in tax receipts, at least 4% of GDP.
(7) Only those derivatives with a free amino group and net positive charge in the side chain were effective.
(8) Charge data from the target hospital showed a statistically significant reduction in laboratory charges per patient in the quarter following program initiation (P = 0.02) and no evidence for change in a group of five comparison hospitals.
(9) At a fixed concentration of nucleotide the effectiveness of elution was proportional to the charge on the eluting molecule.
(10) [125I]AaIT was shown to cross the midgut of Sarcophaga through a morphologically distinct segment of the midgut previously shown to be permeable to a cytotoxic, positively charged polypeptide of similar molecular weight.
(11) For retrospective action to be taken, and an FA charge to follow, the decision of the panel must be unanimous.” The match between the sides ended in acrimony and two City red cards.
(12) As a Native American I am pretty sensitive to charges of racism and white supremacy,” the Oklahoma congressman added.
(13) Under a revised deal most people are now being vetted on time, but charges for the service have had to rise from £12 and free vetting for volunteers, to £28 for a standard disclosure and £33 for an advanced disclosure.
(14) Four Dutch activists were charged in Murmansk this week under the law.
(15) Both polycations investigated reduced the negative surface charge of assay cells and enhanced in vitro infectivity of murine C-type viruses, but had no influence on leukemia-virus-induced XC cell syncytia formation.
(16) The charges against Harrison were filed just after two white men were accused of fatally shooting three black people in Tulsa in what prosecutors said were racially motivated attacks.
(17) The antibody-hapten profiles revealed that the DNCB-fed animalss contained predominatly IgG2 in their serum by the time of their initial bleedings, whereas sensitized animals still contained a considerable proportion of more acidic antibodies having marked charge heterogeneity.
(18) With the flat-fee system, drug charges are not recorded when the drug is dispensed by the pharmacy; data for charging doses are obtained directly from the MAR forms generated by the nursing staff.
(19) As calls grew to establish why nobody stepped in to save Daniel, it was also revealed that the boy's headteacher – who saw him scavenging for scraps – has not been disciplined and has been put in charge of a bigger school.
(20) The phenomenon can be ascribed to the decrease in charge density due to the incorporation of dodecyl alcohol into SDS micelles.
Primer
Definition:
(n.) One who, or that which, primes
(n.) an instrument or device for priming; esp., a cap, tube, or water containing percussion powder or other compound for igniting a charge of gunpowder.
(a.) First; original; primary.
(n.) Originally, a small prayer book for church service, containing the little office of the Virgin Mary; also, a work of elementary religious instruction.
(n.) A small elementary book for teaching children to read; a reading or spelling book for a beginner.
(n.) A kind of type, of which there are two species; one, called long primer, intermediate in size between bourgeois and small pica [see Long primer]; the other, called great primer, larger than pica.
Example Sentences:
(1) The following conclusions emerge: (i) when the 3' or the 3' penultimate base of the oligonucleotide mismatched an allele, no amplification product could be detected; (ii) when the mismatches were 3 and 4 bases from the 3' end of the primer, differential amplification was still observed, but only at certain concentrations of magnesium chloride; (iii) the mismatched allele can be detected in the presence of a 40-fold excess of the matched allele; (iv) primers as short as 13 nucleotides were effective; and (v) the specificity of the amplification could be overwhelmed by greatly increasing the concentration of target DNA.
(2) Elongation of existing RNA primers by the human polymerase-primase was semi-processive; following primer binding the DNA polymerase continuously incorporated 20 to 50 nucleotides, then it dissociated from the template DNA.
(3) This cDNA was obtained because of an identical 10 bp match with the 3' end of one of the GnRH primers.
(4) When PCR products in each of the 12 cats were subjected to a second amplification using the same primer pair (two-step amplification: double PCR), FIV proviral DNA was detected in all of the cats.
(5) In junctions, 3' PSS termini are preserved by fill-in DNA synthesis, although their 5' recessed ends cannot serve as a primer.
(6) Previous studies demonstrated that, when poly(dT).oligo(dA) was used as a template-primer, both proteins were required for poly(dA) synthesis.
(7) 5'-Ends of transcripts of the region were located by S1 nuclease mapping and primer extension.
(8) Nucleotide incorporation kinetics were determined and sequence specific pausing was analyzed by primer-extension.
(9) Primer extension and S1 nuclease protection analysis demonstrate that the hepatic and kidney specific mRNAs are transcriptionally initiated at different sites within the sialyltransferase gene.
(10) alpha 1 and alpha 2 were very similar as DNA polymerases in their sensitivity to several inhibitors and their preference for template-primers, except that alpha 1 had a slightly greater preference for poly (dT) X (rA)10 than alpha 2 did.
(11) Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5'-flanking DNA sequences.
(12) Cognate sites in genomes that diverged approximately 100 million years ago can be detected by PCR assays based on primer pairs from unique sequences.
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) This reaction involved the synthesis of a short oligoribonucleotide primer which was then extended into a DNA chain.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) B.subtilis phage M2 uses a protein, instead of RNA, as the primer of its DNA replication.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) Primer extension experiments show that in fission yeast transcripts are initiated at the same starting point as in tomato, indicating for the first time that a plant promoter can be correctly recognized in fission yeast.
(19) Genomic clones for the mouse estrogen receptor have been isolated from a cosmid library and used in conjunction with the cDNA clones to study the expression of the receptor in vivo by RNase mapping, primer extension, and Northern blotting.
(20) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.