(1) The procedure involves sonication of crude membrane preparations anaerobically in 0.1 M 2-(N-cyclohexylamino)ethane-sulfonic acid (CHES) buffer (pH 8.6-9.0) containing 2 mM dithiothreitol.
(2) It is suggested that the nonesteratic oxadiazolidinones are converted to carbanillates on the surface of the enzyme, then acylate the active site of ChEs, producing carbanillated enzymes.
(3) Here, this procedure was used in order to investigate the recovery of ChEs in the two pools after irreversible inhibition by soman which was directly injected into the ganglion.
(4) Anticholinesterase medications (anti-ChEs) play a significant role in the diagnosis and treatment of myasthenia gravis (MG).
(5) Comparing in innervated and decentralized ganglia the pattern of recovery rate and ultrastructural reappearance of ChEs after local inhibition, with that reported after systemic ChEs inhibition, it was possible to distinguish between the indirect effects of innervation on the recovery rate and pattern of ChEs of ganglion origin and the direct contribution to the total ganglion enzyme activity of ChEs originating in the preganglionic elements.
(6) The slow negative potential was enhanced by anti-ChEs, eliminated by ACh and nicotine, and unaffected by atropine.5.
(7) The effects of anticholinesterases (anti-ChEs) (physostigmine, prostigmine and TEPP) on the afterdischarges and the extracellular and intracellular slow potentials of bullfrog sympathetic ganglia were studied.2.
(8) Two modifications of Lowry's procedure (Markwell's and Kresze's) were investigated for interference by zwitterionic buffers) (HEPES, HEPPS, PIPES, MES, MOPS, CHES, CAPS, Tris, glycylglycine, bicine), detergents (Triton-X-100, digitonin), sugars (sucrose, mannitol, sorbitol), salts (NaCl, KCl, ammonium acetate), chelators (EDTA), and reducing agents (dithiothreitol, GSH, ascorbic acid).
(9) Results obtained from these systematic studies demonstrate that: (a) a quantitative linear correlation exists between blood AChE levels and the protection afforded by exogenously administered ChEs in animals challenged with OP, (b) approximately one mole of either AChE or BChE sequesters one mole of OP, (c) such prophylactic measures are sufficient to protect animals against OPs without the administration of any supportive drugs.
(10) These observations indicate that structural interactions exist between N' and C' terminal domains in CHEs which contribute to substrate and inhibitor binding and suggest a crucial involvement of both electrostatic and hydrophobic domains in the build-up of the CHE active center.
(11) Actinomycyn D decreased the rate of initial rapid phase of recovery of intracellular ChEs when injected in the ganglion daily for three days, whereas the recovery of extracellular ChEs was already decreased the first day of Actinomycyn D application.
(12) We have previously described the procedure for quantitative separation of extracellular and intracellular ChEs using mild treatment of rat superior cervical ganglion with papain.
(13) Cholinesterases (ChEs) are highly polymorphic proteins, capable of rapidly hydrolyzing the neurotransmitter acetylcholine and involved in terminating neurotransmission in neuromuscular junctions and cholinergic synapses.
(14) Email from Ches Schnider entitled "Greetings from Bangkok" Your broadcast is the only way to get the game from over here.
(15) The anti-ChEs augmented the early afterdischarge, the late negative potential and the slow excitatory postsynaptic potential.
(16) After such treatment only ganglion ChEs were totally inhibited, whereas the activity of ChEs in preganglionic neurons and their axons remained unaffected.
(17) The finding of two sites for ChEcDNA hybridization suggests that the two loci coding for human ChEs may include nonidentical sequences responsible for the biochemical differences between ChE variants.
(18) Wild-type che genes of E. coli F'420 complemented all che mutants of Salmonella except cheS, cheV, and a subclass of cheU.
(19) Imidazoles, Ches and phosphate are thought to participate in a metal center-to-buffer proton transfer pathway, whereas Bicine, Taps, Mops and Mes appear to lack this capacity, so that the rate-limiting proton transfer occurs in a metal center-to-bulk water pathway for these variants.
(20) Results using pH 9 CHES buffer show that rh IL-3 is easily separated from a common carrier, human serum albumin, in a commercial preparation.
Pain
Definition:
(n.) Punishment suffered or denounced; suffering or evil inflicted as a punishment for crime, or connected with the commission of a crime; penalty.
(n.) Any uneasy sensation in animal bodies, from slight uneasiness to extreme distress or torture, proceeding from a derangement of functions, disease, or injury by violence; bodily distress; bodily suffering; an ache; a smart.
(n.) Specifically, the throes or travail of childbirth.
(n.) To inflict suffering upon as a penalty; to punish.
(n.) To put to bodily uneasiness or anguish; to afflict with uneasy sensations of any degree of intensity; to torment; to torture; as, his dinner or his wound pained him; his stomach pained him.
(n.) To render uneasy in mind; to disquiet; to distress; to grieve; as a child's faults pain his parents.
Example Sentences:
(1) Experience of pain is modified by intern and extern influences, and it can appear very multiformly in the chronicity.
(2) All subjects completed the Coping Strategies Questionnaire, which measures the use and perceived effectiveness of a variety of cognitive and behavioral coping strategies in controlling and decreasing pain.
(3) Although solely nociresponsive neurons are clearly likely to fill a role in the processing and signalling of pain in the conscious central nervous system, the way in which such useful specificity could be conveyed by multireceptive neurons is difficult to appreciate.
(4) Sixteen patients were operated on for lumbar pain and pain radiating into the sciatic nerve distribution.
(5) Needle acupuncture did, however, increase the pain threshold compared with the initial value (alpha = 0.1%).
(6) Pain is not reported in the removal area, the clinical examinations show identical findings on both patellar tendons, X-ray and ultrasound evaluations do not demonstrate any change in patellar position.
(7) For assessment of clinical status, investigators must rely on the use of standardized instruments for patient self-reporting of fatigue, mood disturbance, functional status, sleep disorder, global well-being, and pain.
(8) However, as the plan unravels, Professor Marcus's team turn on one another, with painfully (if painfully funny) results.
(9) During the chronic phase, pain was assessed using visual analogue scales at 8 AM and 4 PM daily.
(10) Symptoms, particularly colicky abdominal pain, improved during the period of chelation therapy.
(11) Cook, who has postbox-red hair and a painful-looking piercing in his lower lip, was now on stage in discussion with four fellow YouTubers, all in their early 20s.
(12) The main clinical symptom was pain, usually sciatica, while neurological symptoms were less common than they are in adults.
(13) The study revealed that hypophysectomy and ventricular injection of AVP dose dependently raised pain threshold and these effects were inhibited by naloxone.
(14) Anxious mood and other symptoms of anxiety were commonly seen in patients with chronic low back pain.
(15) During these delays, medical staff attempt to manage these often complex and painful conditions with ad hoc and temporizing measures,” write the doctors.
(16) In this study, a potassium nitrate-polycarboxylate cement was used as a liner and was found clinically to tend to preserve pulpal vitality and significantly eliminate or decrease postoperative pain.
(17) The successful treatment of the painful neuroma remains an elusive surgical goal.
(18) Univariate and multivariate analyses indicated previous LBP or back pain in another location of the spine were strongly associated with LBP during the study year.
(19) Our previous study demonstrated that acupuncture increased pain threshold of the body, especially in the inflammatory area.
(20) The triad of epigastric pain unrelieved by antacids, bilious vomiting, and weight loss, particularly after a gastric operation should make one suspect this syndrome.