(1) Blood samples were analysed by mass spectroscopy and gas chromatography.
(2) Previous attempts to purify this enzyme from the liquid endosperm of kernels of Zea mays (sweet corn) were not entirely successful owing to the lability of partially purified preparations during column chromatography.
(3) ASF-II was purified to apparent homogeneity by using concanavalin A-agarose affinity chromatography, DEAE-cellulose chromatography, alumina gel adsorption, and isoelectric focussing techniques.
(4) The overall recoveries of activated ER following chromatography on DEAE-cellulose were significantly lower than the recoveries of the nonactivated ER, 71 and 85%, respectively.
(5) The enzyme was solubilized by Triton X-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-AH-Sepharose 4B.
(6) Glycosyl ceramide concentration was determined by gas-liquid chromatography of the trimethylsilyl ethers of the methyl glycosides.
(7) The sensitivity of an indirect fluorescent antibody (IFA) test (screening test) for the detection of antibodies to cytomegalovirus (CMV) was examined by using 128 serum specimens and quaternary aminoethyl (QAE)-Sephadex A50 column chromatography to separate IgM from IgG class antibodies.
(8) Chromatography and immunoassays are the two principal techniques used in research and clinical laboratories for the measurement of drug concentrations in biological fluids.
(9) We have compared two new methods (a solvent extraction technique and a method involving a disposable, pre-packed reverse phase chromatography cartridge) with the standard method for determining the radiochemical purity of 99Tcm-HMPAO.
(10) 2,3-Dihydroxybenzamide had previously been detected only as a minor metabolite of salicylamide by paper chromatography.
(11) Sephadex LH-20 column chromatography was used for the separation of the steroid prior to assay.
(12) Ether extracts were analyzed by gas chromatography-mass spectrometry (GC-MS) and various chlorinated and non-chlorinated compounds were detected, e.g.
(13) The free nucleoside IV was obtained by removal of blocking groups by sodium methoxide catalyzed deacylation, deionization under reducing atmosphere, and chromatography on neutral alumina.
(14) High pressure liquid chromatography combined with radioimmunoassay showed marked heterogeneity of SPLI and SLI.
(15) Glycoproteins were isolated by lectin affinity chromatography or by elution from nitrocellulose membranes.
(16) Thus Sephadex chromatography of the solution obtained by dissolving the antigen-antibody precipitate in these media repeatedly gave two peaks corresponding to anti-ovalbumin and ovalbumin.
(17) Separation of PL by thin-layer chromatography revealed a prevalence of phosphatidylcholine followed by phosphatidylethanolamine.
(18) A rapid method is described for the purification and analysis of synthetic oligonucleotides, based on reversed-phase high-performance liquid chromatography.
(19) These analyses were carried out on unfractionated culture fluids and on fractions obtained by fast protein liquid chromatography separation using Superose 6 gels.
(20) The corresponding hydrides, mono-n-butyltin hydride, di-n-butyltin hydride, tri-n-butyltin hydride, monophenyltin hydride, diphenyltin hydride triphenyltin hydride, are detected by electron-capture gas chromatography after clean-up by silica gel column chromatography.
Pellicular
Definition:
(a.) Of or pertaining to a pellicle.
Example Sentences:
(1) The calmodulin-dependent guanylate cyclase of Tetrahymena pyriformis was shown previously to be localized in surface membranes (ciliary and pellicular membranes) (Kudo, S, Nakazawa, K, Nagao, S & Nozawa, Y, Japan j exp med 52 (1952) 193) [21], whereas in a recent report Schultz et al, (Schultz, J E, Schonefeld, U & Klumpp, S, Eur j biochem 137 (1983) 89) [12] demonstrated the localization of this enzyme in ciliary membrane, arguing against its presence in pellicular membrane.
(2) We have been able to use immunogold labeling with monoclonal antibodies specific for tyrosinated alpha-tubulin to define new microtubule assembly within the T. brucei pellicular cytoskeleton.
(3) There was evidence of a redistribution of the CS antigens away from the pellicular region of the sporozoite.
(4) The enzyme was either in free solution or immobilized on a pellicular support which consisted of a porous carbonaceous layer on solid glass beads.
(5) Six fracture faces, derived from the plasma membrane and 2 pellicular membranes, have been identified at the surface of the free merozoite.
(6) After incorporation of the drug or dosage form in a solvent mixture and addition of an internal standard, tribenzylamine, an aliquot is chromatographed using a pellicular silica gel medium followed by UV spectrophotometric detection at 254 nm.
(7) It has no detectable microfilaments under the plasma membrane, except at the desmosomes in the region of flagellar binding, and it also has a pellicular cortex of microtubules.
(8) The outer and inner pellicular membranes are uniformly spaced and connected by "cross-bridges."
(9) This process was characterised by the occurrence of subpellicular microtubules exclusively in the anterior half of the sporozoite, by a degeneration of the 2 inner pellicular membranes, by an outpocketing of the parasite's surface, and by the arrangement of microtubules in clusters.
(10) Excess pellicular microtubules are formed after treatment of trypanosomes with NCS, suggesting that in trypanosomes the microtubules replicate by induction, not by division.
(11) Triethylaminoethyl cellulose gave somewhat better resolution of PGA2 and PGB2 as compared with the pellicular strong anion-exchange column, but the advantage of this effect is offset by the tediousness of packing columns of triethylaminoethyl cellulose.
(12) The structure of the sub-pellicular microtubules appears well defined and it was possible to distinguish the 13 protofilaments which compose the microtubule wall.
(13) Thus, both stalk and body contractile bundles have linkage complexes that link their associated membrane systems to the microfibrils and, in turn, connect this membrane-microfibrillar interface to the pellicular membranes.
(14) Infections of DS cell and mouse peritoneal macrophage cultures showed amastigotes with a high ribosomal density and deep invaginations of the pellicular layer.
(15) In this way pellicular anion chromatography in combination with this postcolumn reaction reaction to be a sensitive and specific HPLC procedure for analysis of monosaccharides and oligosaccharides in complex biological matrices.
(16) A monoclonal antibody (McAb 3C3) reacting with a pellicular antigen of Eimeria nieschulzi sporozoites has been selected among hybridomas produced against this organism by immunofluorescence assay.
(17) Soon after this, pellicular microtubules disappeared without change of axonemal microtubules.
(18) Areas of close proximity between cells and the pellicular material were seen and were interpreted as adherence junctions.
(19) The released, depolymerized, pellicular tubulin from T. brucei cross-reacted with a monoclonal antibody raised against yeast tubulin.
(20) The ultrastructure of the cell envelope was not greatly affected by carbon source or by growth habit except that a slime layer was more prevalent in pellicular cells compared with those grown in submerged culture.