What's the difference between chromogen and dye?

Chromogen


Definition:

  • () Vegetable coloring matter other than green; chromule.
  • () Any colored compound, supposed to contain one or more chromophores.

Example Sentences:

  • (1) It includes preincubation of diluted plasma with ellagic acid and phospholipids and a starting reagent that contains calcium and a chromogenic peptide substrate for thrombin, Tos-Gly-Pro-Arg-pNA.
  • (2) The commercially available chromogenic p-nitroanilide substrates Pro-Phe-Arg-NH-Np (S2302 or chromozym PK), Glp-Pro-Arg-NH-Np (S2366), Ile-Glu-(piperidyl)-Gly-Arg-NH-Np (S2337), and Ile-Glu-Gly-Arg-NH-Np (S2222) were tested for their suitability as substrates in these assays.
  • (3) It has now achieved wide applicability to the measurement of cellular biochemical activity by means of stoichiometric chromogenic reactions.
  • (4) The turnover of fluorogenic substrates is lower for all enzymes than the turnover of chromogenic substrates but due to the more sensitive detection of fluorogenic products the detection limits for all conjugates were lowered too--especially for beta Gal-IgG by a factor of 333 compared to the colorimetric procedure.
  • (5) Methods based on the application of chromogenic and fluorogenic substrates enable specific and rapid detection of a variety of bacterial enzymatic activities.
  • (6) Fluorogenic 6-octanoyl- and 6-hexadecanoyl-amino-4-methylumbelliferyl beta-D-glucopyranoside were much less readily hydrolyzed by both human and animal glucocerebrosidase than chromogenic 2-hexadecanoylamino-4-nitrophenyl beta-D-glucopyranoside.
  • (7) Diethyldithiocarbamate did not influence the effects of NiCl2 on TBA-chromogens in liver homogenates or on serum AST and ALT activities but acted synergistically with NiCl2 to diminish serum alkaline phosphatase activity and to increase serum bilirubin concentration.
  • (8) The newly developed endotoxin specific chromogenic Limulus assay (endospecy test), which is devoid of factor G, did not react with any rabbit serum.
  • (9) Substratum-bound enzyme could be solubilized in buffers containing sodium dodecyl sulfate (SDS) or Triton X-100 and demonstrated by zymography following electrophoresis or assayed for amidolytic activity with a chromogenic substrate (Kabi S-2251).
  • (10) The released glycerol is oxidized with glycerol dehydrogenase from Erwinia aroideae in the presence of NAD+, were the reduction of the enzyme-linked NAD+ is coupled to the reduction of nitro blue tetrazolium as a chromogenic indicator with phenazine methosulfate serving as an intermediate electron carrier of NADH.
  • (11) The chromogenic substrate, CPRG (chlorophenol red-beta-D-galactopyranoside), was compared with ONPG (o-nitrophenyl-beta-D-galactopyranoside) by kinetic analysis with purified beta-galactosidase.
  • (12) Using peptide 4-methoxy-2-naphthylamide substrates (Bz-Arg-MNA, Z-Lys-Arg-MNA, Z-Gly-Arg-MNA, Z-Pro-Arg-MNA and Z-Gly-Pro-Arg-MNA) and Fast Garnet GBC as chromogen the red azo dye was found to precipitate in the cytoplasmic granules of the cutaneous mast cells.
  • (13) The continuous chromogenic tissue factor assay has been compared to one-stage and two-stage coagulation assays, and its sensitivity has been established using purified human placental tissue factor.
  • (14) Cleavage of chromogenic peptidyl substrate by TFA165A166.VIIa complexes was not diminished, demonstrating that TFA165A166 supported enhancement of catalytic function of the VIIa protease domain.
  • (15) Factor X-activating activity (FXAA) was determined by a chromogenic assay in normal and malignant breast tissue.
  • (16) Diaminobenzidine and tetramethylbenzidine were used as chromogens to distinguish ChAT (protein) and VIP (peptide) immunoreactivities.
  • (17) In an enzyme immunoassay for anti-Salmonella antibodies using glucose oxidase as the indicator enzyme, the molybdate-enhanced polyvinyl alcohol- and starch-glucose-iodide substrate systems were as sensitive as a conventional glucose oxidase assay system employing horseradish peroxidase as a secondary enzyme and a suitable hydrogen-donating chromogen.
  • (18) strain 3707, a chromogenic rapid grower; C72 to C78 (av.
  • (19) 4. alpha-D-mannosidase was the most active with both fluorescent and chromogenic substrates.
  • (20) A sample containing fibrin is incubated with t-PA, plasminogen and a plasmin-sensitive chromogenic substrate.

Dye


Definition:

  • (v. t.) To stain; to color; to give a new and permanent color to, as by the application of dyestuffs.
  • (n.) Color produced by dyeing.
  • (n.) Material used for dyeing; a dyestuff.
  • (n.) Same as Die, a lot.

Example Sentences:

  • (1) The most successful dyes were phenocyanin TC, gallein, fluorone black, alizarin cyanin BB and alizarin blue S. Celestin blue B with an iron mordant is quite successful if properly handled to prevent gelling of solutions.
  • (2) The actions of the polyvalent cationic dye Ruthenium Red and the enzyme neuraminidase were studied at the frog neuromuscular junction.
  • (3) Significant increases in the extravasation of dye were observed in both animal groups sensitized with IgG1 and IgG2 antibodies.
  • (4) While the reduced form of the "derived" polyphenolic compounds, generated during tissue homogenization, appeared to enhance dye binding with bovine serum albumin, their influence on the protein assay directly in crude homogenates was extremely diverse.
  • (5) To selectively stain polyanionic macromolecules of growth plate cartilage and to prevent artifacts induced by aqueous fixation, proximal tibial growth plates were excised from rats, slam-frozen, and freeze-substituted in 100% methanol containing the cationic dye Alcian blue.
  • (6) This dye is concentrated and secreted by the parietal cells.
  • (7) The rhodamine 123-induced growth inhibition was partially reversed by treating the dye-pre-exposed infected erythrocytes with the proton ionophore carbonyl-cyanide m-chlorophenylhydrazone, which dissipates transmembrane proton gradients.
  • (8) The duration of electrophoresis was based on the migration of a marker dye for a predetermined distance.
  • (9) Effects of fixation with glutaraldehyde (GA), glutaraldehyde-osmium tetroxide (GA-OsO(4)), and osmium tetroxide (OsO(4)) on ion and ATP content, cell volume, vital dye staining, and stability to mechanical and thermal stress were studied in Ehrlich ascites tumor cells (EATC).
  • (10) By using pH- or Ca(2+)-sensitive dyes and recording at the ion-sensitive and -insensitive (isosbestic) wavelengths, the method can measure both cell volume changes and intracellular ionic activities.
  • (11) An argon dye laser system with lambda em=630 nm (400 mW cm-2) was used for PDT with a total light dose of 400 J cm-2.
  • (12) In a complete system, consisting of a dye-donor couple, ferredoxin, thioredoxin and ferredoxin-thioredoxin reductase, light activation of purified spinach fructose-1,6-bisphosphatase was observed in vitro.
  • (13) When given 30 min after acetic acid instillation SC-41930 prevented the rise in myeloperoxidase and dye extravasation observed in the acetic acid inflammed tissue.
  • (14) A comparison was made between the Q's estimated by the CO2 rebreathing method during tethered swimming and previously published data on Q determined by the dye-dilution method during free swimming in a flune.
  • (15) A novel staining procedure for enumerating osteoclasts on neonatal mouse calvaria with the vital fluorescent dye acridine orange is described.
  • (16) Thus, angiographic dye appears to decrease heart rate by a direct effect on pacemaker tissue and by reflex vagal suppression of the sinus pacemaker.
  • (17) The purpose of this study was to compare the level of apical dye penetration when different sealers were used.
  • (18) This protein which we call CBP-58 bears similarities to the endoplasmic reticulum protein, calreticulin, in that it has a pI of 4.7 containing approximately 30% glutamate and aspartate, has a high capacity for calcium, and stains blue with the carbocyanine dye, 'Stains-all'.
  • (19) Minced and triturated fragments from the spinal cord of normal rat fetuses (15-18 days gestation) labeled with the fluorescent dye fast blue (FB) were successfully transplanted into juvenile myelin-deficient rat spinal cord under direct observation.
  • (20) In vitro, the soluble core PEI and membrane both bound reactive substances of limited aqueous stability, such as from [14C]N-methyl-N-nitrosourea ([14C]NMU), and aqueous stable dyes of molecular weight up to 1000.

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