(1) The amino acid sequence deduced from the nucleotide sequence contained both amino- and carboxyl-terminal sequences.
(2) The liver metastasis was produced by intrasplenic injection of the fluid containing of KATOIII in nude mouse and new cell line was established using the cells of metastatic site.
(3) In contrast, resting cells of strain CHA750 produced five times less IAA in a buffer (pH 6.0) containing 1 mM-L-tryptophan than did resting cells of the wild-type, illustrating the major contribution of TSO to IAA synthesis under these conditions.
(4) The nucleotide sequence of a 2.2-kb DNA fragment which contains the complete RAD7 gene was determined.
(5) As a consequence, similar response curves were obtained for urine specimens containing morphine or barbiturates.
(6) IT can, therefore, be excluded almost with certainty that the meat would contain such large amounts of hormone residues.
(7) The extent of the infectious process was limited, however, because the life span of the cultures was not significantly shortened, the yields of infectious virus per immunofluorescent cell were at all times low, and most infected cells contained only a few well-delineated small masses of antigen, suggestive of an abortive infection.
(8) In addition to oncogenes, the transferred DNA contains genes that direct the synthesis and exudation of opines, which are used as nutrients by the bacteria.
(9) These studies, in addition to demonstrating that the placenta contains TRH deamidase activity, suggest that losses of fetal TRH through the placenta are not large.
(10) Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive.
(11) The human placental villus tissue contains opioid receptors and peptides.
(12) Gel filtration of the 40,000 rpm supernatant fraction of a homogenate of rat cerebral cortex on a Sepharose 6B column yielded two fractions: fraction II with the "Ca(2+) plus Mg(2+)-dependent" phosphodiesterase activity and fraction III containing its modulator.
(13) These cells contained organelles characteristic of the maturation stage ameloblast and often extended to the enamel surface, suggesting a possible origin from the ameloblast layer.
(14) It includes preincubation of diluted plasma with ellagic acid and phospholipids and a starting reagent that contains calcium and a chromogenic peptide substrate for thrombin, Tos-Gly-Pro-Arg-pNA.
(15) The p60v-src protein encoded by Prague Rous sarcoma virus was found to contain two sites of tyrosine phosphorylation.
(16) The 68C intermolt puff of Drosophila melanogaster contains a cluster of three glue protein genes, Sgs-3, Sgs-7, and Sgs-8.
(17) The deduced amino acid sequence contained no consensus sequence indicative of N-glycosylation.
(18) Maximal yields of lipid and aflatoxin were obtained with 30% glucose, whereas mold growth, expressed as dry weight, was maximal when the medium contained 10% glucose.
(19) This analysis demonstrated that more than 75% of cosmids containing a rare restriction site also contained a second rare restriction site, suggesting a high degree of CpG-rich restriction site clustering.
(20) The region containing the injection stop signal (iss) has been cloned and sequenced and found to contain numerous large repeats and inverted repeats which may be part of the iss.
Flask
Definition:
(n.) A small bottle-shaped vessel for holding fluids; as, a flask of oil or wine.
(n.) A narrow-necked vessel of metal or glass, used for various purposes; as of sheet metal, to carry gunpowder in; or of wrought iron, to contain quicksilver; or of glass, to heat water in, etc.
(n.) A bed in a gun carriage.
(n.) The wooden or iron frame which holds the sand, etc., forming the mold used in a foundry; it consists of two or more parts; viz., the cope or top; sometimes, the cheeks, or middle part; and the drag, or bottom part. When there are one or more cheeks, the flask is called a three part flask, four part flask, etc.
Example Sentences:
(1) Plaque size, appearance, and number were influenced by diluent, incubation temperature after nutrient overlay, centrifugation of inoculated tissue cultures, and number of host cells planted initially in each flask.
(2) In both media the DNA and protein content of cultures kept for 3-5 days in the presence of 80-800 nM Buserelin and 1 nM oestradiol were 8-27% lower than those of flasks cultured in the presence of oestradiol alone (P less than 0.05).
(3) The vacuum flask method of using boiling water to decontaminate soft contact lenses is better and less expensive than other ways of using moist heat and can be safely and effectively applied under most domestic circumstances.
(4) With 1-octanol-saturated buffers as mobile phases, a stable baseline (compared to 1-octanol adsorbed on silica) is obtained rapidly, and the log relative retention times are highly correlated with unit slope to log distribution or partition coefficients obtained from the classical shake-flask procedures.
(5) Bacterial and plasmid yields have been shown to be equal to or greater than those obtained using conventional shake flasks.
(6) The carbohydrate compounds of the mucus of flask cells in the kidney of claw-frogs (Xenopus laevis) were studied by gold marked lectins (WGA, RCA, L, LCA, HPA, PNA).
(7) Irradiated melanomas did not grow and did not attach to culture flasks, thus demonstrating that preenucleation irradiation alters the in vitro growth of melanoma cells.
(8) An orbital shaker was used to create a water current in 250-ml Erlenmeyer flasks containing the test larvae.
(9) The cuffed end of each tracheal tube was inserted into the neck of an empty flask, and the tube and flask were flushed with oxygen for 5 min before cuff inflation.
(10) Incubations were carried out in sealed flasks under oxygen concentrations ranging from 0.14 to 21% at 37 degrees.
(11) The reverse difference in the temperatures (higher temperature of the culture flasks) leads to the formation of the reverse pattern of the cell layer, with higher density corresponding to the holes.
(12) By the use of double-chamber tissue culture flasks, with the 2 cell populations separated by a cell impermeable membrane, it was found that T-T interaction does not require cell contact and is thus mediated by factor(s).
(13) Everyone knows that Father Christmas’s tipple of choice is brandy, so Santa, if you’re reading this, we recommend you pause in The Flask on Highgate West Hill for a quick snifter.
(14) The tumor specimens were minced into fragments approximately 1 mm in diameter and cultured in plastic culture flasks in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS) and 50% patients serum.
(15) Oxygen diffusion distance was measured in solid tumor "cubes" prepared by excising the tumor from the mouse and incubating 1-2 mm sided tumor cubes in spinner culture flasks with fluorescent drugs (AF-2 or DM113) which bind to hypoxic cells.
(16) The growth-promoting effect on haemopoietic cells seems to be independent of the number of marrow cells per culture flask initially inoculated into the cultures to establish the adherent cell layer.
(17) Cells were cultured in Roux flasks in HAM's F-12 medium, and the pH was varied with the final medium change.
(18) The procedure involved the transfer of heavy mold-form inocula to flasks that contained small volumes of brain heart infusion broth.
(19) We have recently shown that the semi-continuous cultivation of a mouse hybridoma line in spinner flasks, with a basal defined medium (BDM) devoid of serum and protein, increases the secretion of the immunoreactive monoclonal antibody (MAb) by a factor of ca.
(20) Cultured human adrenal cortical adenocarcinoma cells (SW-13) form a confluent monolayer of epithelial-like cells when seeded into culture flasks.